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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BmNP01_FL5_B02
         (788 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_14332| Best HMM Match : Ribosomal_L3 (HMM E-Value=8.6e-34)         212   3e-55
SB_51931| Best HMM Match : Ribosomal_L3 (HMM E-Value=0)               208   3e-54
SB_55406| Best HMM Match : DUF1534 (HMM E-Value=0.45)                  29   5.7  
SB_25655| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.7  
SB_14298| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.5  
SB_54689| Best HMM Match : DUF1525 (HMM E-Value=6.3)                   28   9.9  
SB_36686| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   9.9  

>SB_14332| Best HMM Match : Ribosomal_L3 (HMM E-Value=8.6e-34)
          Length = 347

 Score =  212 bits (517), Expect = 3e-55
 Identities = 92/125 (73%), Positives = 109/125 (87%)
 Frame = +1

Query: 22  LSSSMSHRKFSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKSVHLTAFIGYKAGMTHV 201
           L   MSHRKF APRHGS+GF P+KR +RHRGKVK+FPKDD +   HLTAFIG+KAGMTH+
Sbjct: 44  LEPKMSHRKFEAPRHGSLGFLPRKRCKRHRGKVKSFPKDDNTLPPHLTAFIGFKAGMTHI 103

Query: 202 VREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRR 381
           +RE ++PGSK+NKKE VEAVTIIETPPM+ VGVVGYIETP G+R L T+WAEH+SE+C+R
Sbjct: 104 LREVEKPGSKLNKKEKVEAVTIIETPPMMVVGVVGYIETPRGMRVLKTIWAEHLSEECKR 163

Query: 382 RFYKN 396
           RFYKN
Sbjct: 164 RFYKN 168



 Score = 48.0 bits (109), Expect = 9e-06
 Identities = 19/31 (61%), Positives = 24/31 (77%)
 Frame = +1

Query: 580 WQDELGRKSIEKDFKKMIRYCSVVRVIXHTQ 672
           W D+ G+KSIE+DF  M +YC V+RVI HTQ
Sbjct: 184 WADDDGKKSIEEDFNTMKKYCKVIRVICHTQ 214


>SB_51931| Best HMM Match : Ribosomal_L3 (HMM E-Value=0)
          Length = 338

 Score =  208 bits (509), Expect = 3e-54
 Identities = 90/120 (75%), Positives = 107/120 (89%)
 Frame = +1

Query: 37  SHRKFSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKSVHLTAFIGYKAGMTHVVREPD 216
           SHRKF APRHGS+GF P+KR +RHRGKVK+FPKDD +   HLTAFIG+KAGMTH++RE +
Sbjct: 1   SHRKFEAPRHGSLGFLPRKRCKRHRGKVKSFPKDDNTLPPHLTAFIGFKAGMTHILREVE 60

Query: 217 RPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRRRFYKN 396
           +PGSK+NKKE VEAVTIIETPPM+ VGVVGYIETP G+R L T+WAEH+SE+C+RRFYKN
Sbjct: 61  KPGSKLNKKEKVEAVTIIETPPMMVVGVVGYIETPRGMRVLKTIWAEHLSEECKRRFYKN 120



 Score = 73.7 bits (173), Expect = 2e-13
 Identities = 32/46 (69%), Positives = 37/46 (80%)
 Frame = +1

Query: 580 WQDELGRKSIEKDFKKMIRYCSVVRVIXHTQMKLLKQRQKKAHIME 717
           W D+ G+KSIE+DF  M +YC V+RVI HTQ KLLK RQKKAHIME
Sbjct: 136 WADDDGKKSIEEDFNTMKKYCKVIRVICHTQQKLLKMRQKKAHIME 181


>SB_55406| Best HMM Match : DUF1534 (HMM E-Value=0.45)
          Length = 248

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 31/112 (27%), Positives = 39/112 (34%), Gaps = 4/112 (3%)
 Frame = +1

Query: 4   WHFSLR---LSSSMSHRKFSAPRHGSMGFYPKKRSRRHRGK-VKAFPKDDPSKSVHLTAF 171
           W  SLR    S + S+R  +  R    GF     SR  R   +  +   DP  S      
Sbjct: 129 WRRSLRRQQASFAHSNRGMAHGRRRGRGFGFGNASRHERSSALPGYSTHDPRHSNDRPPR 188

Query: 172 IGYKAGMTHVVREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHG 327
                       EP  P S + +  +  A TI  TPP     V    E PHG
Sbjct: 189 GDSIVTQLEGPNEPPPPYSTLERSPVTIADTIDITPPSYEEAVQSSSEEPHG 240


>SB_25655| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 325

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = +1

Query: 43  RKFSAPRHGSMGFYPKKRSRRHRGK-VKAFPKDDPSKSVH 159
           R+F  P+ G +G Y ++  R H GK +   PK +P +  +
Sbjct: 145 RRFWMPKFGHVGPYSEQVKRDHDGKIIDIIPKGNPMEDAY 184


>SB_14298| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 427

 Score = 28.3 bits (60), Expect = 7.5
 Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
 Frame = -2

Query: 427 NKALIFREESSFCRSDVDSLQTYAP-PRQSTER 332
           N A+I +    +C  D+   Q YAP  RQ++ R
Sbjct: 392 NDAVILKRRECYCEKDLSKTQLYAPSDRQASNR 424


>SB_54689| Best HMM Match : DUF1525 (HMM E-Value=6.3)
          Length = 298

 Score = 27.9 bits (59), Expect = 9.9
 Identities = 11/45 (24%), Positives = 24/45 (53%)
 Frame = +1

Query: 82  YPKKRSRRHRGKVKAFPKDDPSKSVHLTAFIGYKAGMTHVVREPD 216
           +P  ++ +   +V  +P  D    +H+ AF+G+K+  T  +  P+
Sbjct: 70  HPSSQNVKTSLRVPIYPPKDIQVDLHIKAFVGHKSRFTMYIPCPE 114


>SB_36686| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 675

 Score = 27.9 bits (59), Expect = 9.9
 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = +1

Query: 274 TPPMVCVGVVGYIETPHGLRAL-LTVWAEHMSEDCRRRFY 390
           TP  +C+G  GY+ T   L+ L LTV    M E  + +++
Sbjct: 301 TPGRLCIGSYGYVATQQFLQLLRLTVLPPVMIEKAKNQYH 340


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,741,233
Number of Sequences: 59808
Number of extensions: 512499
Number of successful extensions: 1161
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 979
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1161
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2167838629
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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