Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_H09_e456_15.seq
(1527 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 64 6e-11
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 60 1e-09
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 52 3e-07
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 40 0.001
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 35 0.034
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 34 0.060
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 31 0.42
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 30 0.97
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 27 6.8
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 63.7 bits (148), Expect = 6e-11
Identities = 44/150 (29%), Positives = 64/150 (42%), Gaps = 2/150 (1%)
Frame = +2
Query: 5 ADDHKSIAGQYGVSGFPTIKIFTGSKHTPYQGQRTAEAFVDAALKAAKEKAYDNLXXXXX 184
AD H +A +Y ++GFPT+ F P Q + VD+ + EK
Sbjct: 81 ADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARD--VDSLTQFVSEKT-----GIKK 133
Query: 185 XXXXXXXXVITLTDSNFKELVLDSEDLWLVEFYAPWCGHCKNLEPHWAKAATELKGK--V 358
V+ L NF ++V+D + LVEFYA WCG+CK L P + K + V
Sbjct: 134 RKIVLPSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNV 193
Query: 359 KVGCRRCDCASSHGLPLPSAGXXPXMKMFP 448
++ D + G L P +K FP
Sbjct: 194 EIVKINADVFADIG-RLHEVASFPTIKFFP 222
Score = 39.1 bits (87), Expect = 0.002
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +2
Query: 269 LVEFYAPWCGHCKNLEP 319
L+EFYA WCGHCK+L P
Sbjct: 43 LIEFYATWCGHCKSLAP 59
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 59.7 bits (138), Expect = 1e-09
Identities = 26/62 (41%), Positives = 34/62 (54%)
Frame = +2
Query: 209 VITLTDSNFKELVLDSEDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKVGCRRCDCA 388
++ L NF ++V+D LVEFYAPWCGHCKNL P + K A E V + D
Sbjct: 357 LVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDAT 416
Query: 389 SS 394
+
Sbjct: 417 EN 418
Score = 48.8 bits (111), Expect = 2e-06
Identities = 21/53 (39%), Positives = 31/53 (58%)
Frame = +2
Query: 245 VLDSEDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKVGCRRCDCASSHGL 403
++ ++ + +V+FYAPWCGHCK L P + AA EL+ K + DC L
Sbjct: 35 LITADKVLMVKFYAPWCGHCKALAPEYESAADELE-KDGISLVEVDCTEEGDL 86
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 51.6 bits (118), Expect = 3e-07
Identities = 31/102 (30%), Positives = 47/102 (46%), Gaps = 1/102 (0%)
Frame = +2
Query: 212 ITLTDSNFKELVLDSEDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKVGCRRCDCAS 391
I L NF++ V ++ LV FYAPWCG+CK L P + K A+ L + V CD
Sbjct: 34 IELNSKNFRKFV-KAKGPSLVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQ 92
Query: 392 SHGL-PLPSAGXXPXMKMFPSWX*XQATVSEDYXGGRTXQXI 514
+ + P +K+ + S DY G R+ + +
Sbjct: 93 NRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSL 134
Score = 32.3 bits (70), Expect = 0.18
Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 6/41 (14%)
Frame = +2
Query: 5 ADDHKSIAGQYGVSGFPTIK-IFTGSK-----HTPYQGQRT 109
AD ++++ QY V GFPTIK ++ SK T Y G R+
Sbjct: 90 ADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRS 130
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 39.9 bits (89), Expect = 0.001
Identities = 17/50 (34%), Positives = 28/50 (56%)
Frame = +2
Query: 212 ITLTDSNFKELVLDSEDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVK 361
+ LTD++ + V S+ W +++Y P CG CK L P W + K +V+
Sbjct: 29 VPLTDNDLESEV--SKGTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVE 76
Score = 35.5 bits (78), Expect = 0.020
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = +2
Query: 224 DSNFKELVLDSEDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKVGCRRC 379
D++ + D E W ++FY+ C C ++ W A ++GK+ V C
Sbjct: 288 DADIDAALTDKEG-WFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINC 338
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 34.7 bits (76), Expect = 0.034
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +2
Query: 248 LDSEDLWLVEFYAPWCGHCKNLEPHWAKAATE 343
+ ++ + +V+FYA WCG CK L+P K + +
Sbjct: 32 ISADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 33.9 bits (74), Expect = 0.060
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +2
Query: 227 SNFKELVLDSEDLWLVEFYAPWCGHCKNLEP 319
S FK +V + L +V+F+A WCG CK + P
Sbjct: 9 SEFKSIVCQDK-LVVVDFFATWCGPCKAIAP 38
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 31.1 bits (67), Expect = 0.42
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = +2
Query: 233 FKELVLDS-EDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKV 358
F+E++ + E + L+ FYAPW CK + + + A + K V
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAV 53
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 29.9 bits (64), Expect = 0.97
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +2
Query: 272 VEFYAPWCGHCKNLEPHWAKAATE 343
V+ YA WCG CK + P +++ A++
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASK 47
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 27.1 bits (57), Expect = 6.8
Identities = 9/31 (29%), Positives = 15/31 (48%)
Frame = +2
Query: 8 DDHKSIAGQYGVSGFPTIKIFTGSKHTPYQG 100
D+ K + + + FPT ++F G Y G
Sbjct: 238 DEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,698,310
Number of Sequences: 5004
Number of extensions: 35674
Number of successful extensions: 109
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 856299168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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