Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_H05_e424_15.seq
(1582 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 249 9e-67
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 136 8e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 132 2e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 130 5e-31
SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy... 28 3.1
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 5.4
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 5.4
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 7.1
SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa... 27 7.1
SPAPJ760.03c |adg1||sequence orphan|Schizosaccharomyces pombe|ch... 27 7.1
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 9.4
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 27 9.4
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 249 bits (609), Expect = 9e-67
Identities = 109/160 (68%), Positives = 126/160 (78%)
Frame = +1
Query: 154 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 333
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 334 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 513
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 514 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYP 633
R+E+E+CD LQGFQ KIREEYP
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYP 160
Score = 113 bits (272), Expect = 6e-26
Identities = 51/75 (68%), Positives = 61/75 (81%)
Frame = +2
Query: 632 PDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALYDICXRTLKLST 811
PDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CID EAL I TLK+ +
Sbjct: 160 PDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKS 219
Query: 812 PTYGDLNHLVXLXMS 856
P+Y DLNHLV M+
Sbjct: 220 PSYDDLNHLVSAVMA 234
Score = 26.6 bits (56), Expect = 9.4
Identities = 12/35 (34%), Positives = 15/35 (42%)
Frame = +3
Query: 933 VPFPRFPFXXAKICPFHIGGKXNXXXLXXPEXTQK 1037
VPFPR F P G + + PE TQ+
Sbjct: 258 VPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQ 292
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 136 bits (329), Expect = 8e-33
Identities = 62/135 (45%), Positives = 86/135 (63%), Gaps = 2/135 (1%)
Frame = +1
Query: 154 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 327
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 328 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 507
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 508 VVRKESESCDCLQGF 552
+R+ +++C LQGF
Sbjct: 121 KIRRIADNCSGLQGF 135
Score = 71.7 bits (168), Expect = 3e-13
Identities = 30/68 (44%), Positives = 46/68 (67%)
Frame = +2
Query: 653 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALYDICXRTLKLSTPTYGDLN 832
+SV P+P+VS +VVEPYN+ L+ H ++ D T+ +D E+ YDIC R L + P+Y +LN
Sbjct: 169 FSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLN 228
Query: 833 HLVXLXMS 856
L+ +S
Sbjct: 229 RLIAQVVS 236
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 132 bits (318), Expect = 2e-31
Identities = 63/180 (35%), Positives = 97/180 (53%), Gaps = 6/180 (3%)
Frame = +1
Query: 154 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 315
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 316 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 495
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 496 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPRQNHEHILSRPLAQ 675
SVL+ +R+ +++C LQGF ++ EY ++++ P Q
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Score = 78.6 bits (185), Expect = 2e-15
Identities = 33/68 (48%), Positives = 48/68 (70%)
Frame = +2
Query: 653 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALYDICXRTLKLSTPTYGDLN 832
+SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +D EA YDIC R L + PTY +LN
Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLN 232
Query: 833 HLVXLXMS 856
L+ +S
Sbjct: 233 RLIAQVVS 240
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 130 bits (314), Expect = 5e-31
Identities = 57/177 (32%), Positives = 104/177 (58%), Gaps = 2/177 (1%)
Frame = +1
Query: 157 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 336
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 337 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 510
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 511 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPRQNHEHILSRPLAQSI 681
+ +E++ D L+GF ++ + YP++ + P +QS+
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSV 178
Score = 60.9 bits (141), Expect = 5e-10
Identities = 34/86 (39%), Positives = 45/86 (52%), Gaps = 1/86 (1%)
Frame = +2
Query: 602 YLSQRSVKSTPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALY 778
+L +R P +I+ TYSV P S VSD VV+PYN+ L++ +L N D +D AL
Sbjct: 152 FLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALA 211
Query: 779 DICXRTLKLSTPTYGDLNHLVXLXMS 856
I L PT+ N LV MS
Sbjct: 212 HIAADRLHTQNPTFHQQNQLVSTVMS 237
>SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 241
Score = 28.3 bits (60), Expect = 3.1
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +1
Query: 619 REEYPRQNHEHILSRPLAQSIRHRRRTLQCDSL 717
R E+P+ H L++ L + HRR L C++L
Sbjct: 146 RSEFPKNPHA-FLAQDLPHGLPHRRAALPCEAL 177
>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
Mei2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 750
Score = 27.5 bits (58), Expect = 5.4
Identities = 21/86 (24%), Positives = 42/86 (48%), Gaps = 1/86 (1%)
Frame = +2
Query: 395 SDRYSALTTLFSDSPAPVTTGPRDTTQRALS*STQFLT*SEK-NPNHVIVYKDSNSHTLS 571
++ Y +TL P+P+ T+ + S S +LT ++ PN V + N++ S
Sbjct: 45 NEHYPPKSTLLLSGPSPIRNIQLSATKSSESNSIDYLTDTQNIFPNFV---NNENNYQFS 101
Query: 572 EAVPAPVWAHYLSQRSVKSTPDRIMN 649
A P+ A + +R V +T + +++
Sbjct: 102 TAPLNPIDACRVGERKVFTTGNVLLS 127
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 5.4
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 457 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 546
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 7.1
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 193 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 294
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC1289.12 |usp109||U1 snRNP-associated protein
Usp109|Schizosaccharomyces pombe|chr 2|||Manual
Length = 352
Score = 27.1 bits (57), Expect = 7.1
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +2
Query: 617 SVKSTPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVEN 739
SVKS + +++ +P+ + +P N + VHQL EN
Sbjct: 146 SVKSDKNTYLSSPGFYGTPQPLNQFTDPNNTAVYVHQLPEN 186
>SPAPJ760.03c |adg1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 166
Score = 27.1 bits (57), Expect = 7.1
Identities = 25/102 (24%), Positives = 47/102 (46%), Gaps = 1/102 (0%)
Frame = +2
Query: 419 TLFSDSPAPVTTGPRDTTQRALS*STQFLT*SEKNPNHVI-VYKDSNSHTLSEAVPAPVW 595
++F+DS + + P TT + T + SE V+ VY T++ V
Sbjct: 19 SVFADSGVSIVSTPATTTVYLVR--TVDCSSSEVTSQPVVTVYNVLKPDTVTFTVTETAG 76
Query: 596 AHYLSQRSVKSTPDRIMNTYSVVPSPKVSDTVVEPYNATLSV 721
++ ++RS++ D + T + +P S T V Y+A++ V
Sbjct: 77 SY--AKRSIEIDSDSVSPTSATTTTPVASATDVSVYSASIHV 116
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 9.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 388 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 489
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 26.6 bits (56), Expect = 9.4
Identities = 22/67 (32%), Positives = 28/67 (41%)
Frame = +2
Query: 524 PNHVIVYKDSNSHTLSEAVPAPVWAHYLSQRSVKSTPDRIMNTYSVVPSPKVSDTVVEPY 703
P VIV +DS S L+ +P P H L Q V P SP + TV P
Sbjct: 23 PQEVIVIEDSASPRLTPNLPPPFSVHQL-QSFVPPQPPS-------SSSPSTTGTVAVPI 74
Query: 704 NATLSVH 724
N +V+
Sbjct: 75 NGANAVY 81
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,233,900
Number of Sequences: 5004
Number of extensions: 75924
Number of successful extensions: 199
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 891978300
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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