BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030905E5_G06_e431_14.seq (1533 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_24733| Best HMM Match : Shugoshin_C (HMM E-Value=4.2) 30 5.7 SB_1939| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 5.7 SB_9261| Best HMM Match : Chitin_synth_2 (HMM E-Value=2.7e-07) 29 7.5 >SB_24733| Best HMM Match : Shugoshin_C (HMM E-Value=4.2) Length = 689 Score = 29.9 bits (64), Expect = 5.7 Identities = 19/61 (31%), Positives = 32/61 (52%) Frame = +1 Query: 52 RAAGNSARGDESRRRWLPSSASTQLPAGPGRRPTEPSEWPGGRSTRGASRYNIKPTTAAK 231 R N G E+ +P ++S QLP +RP E + G ++ G SR+N+KP+ + Sbjct: 537 RPLDNVPNGSENETERVPGNSS-QLPEVASKRP-EILDIEGAKNP-GNSRFNVKPSAPGE 593 Query: 232 A 234 + Sbjct: 594 S 594 >SB_1939| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1646 Score = 29.9 bits (64), Expect = 5.7 Identities = 18/66 (27%), Positives = 31/66 (46%) Frame = +1 Query: 58 AGNSARGDESRRRWLPSSASTQLPAGPGRRPTEPSEWPGGRSTRGASRYNIKPTTAAKAE 237 +G+S ES + +P+S TQ+ P ++P +PGG S+ Y+I + E Sbjct: 1206 SGSSGSSMESSPQGMPASGKTQITDNPNKQP----GFPGGFSSASQFPYSIASSPWQNTE 1261 Query: 238 EKQASN 255 + N Sbjct: 1262 PTDSLN 1267 >SB_9261| Best HMM Match : Chitin_synth_2 (HMM E-Value=2.7e-07) Length = 2435 Score = 29.5 bits (63), Expect = 7.5 Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 5/60 (8%) Frame = +1 Query: 61 GNSARGDESRRRWLPSSASTQLPAGPGRR-PTEPS----EWPGGRSTRGASRYNIKPTTA 225 G+ GD + + L S++ PG R T PS E+PG R+ R ++ +++PTT+ Sbjct: 1971 GSRLNGDPTSKHDLADFESSRELLAPGERHSTVPSKAACEYPGPRAKRNSAPPDLQPTTS 2030 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 25,798,482 Number of Sequences: 59808 Number of extensions: 385957 Number of successful extensions: 744 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 684 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 742 length of database: 16,821,457 effective HSP length: 85 effective length of database: 11,737,777 effective search space used: 4988555225 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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