Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_G03_e407_13.seq
(1562 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 231 2e-61
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 231 2e-61
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 231 2e-61
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 78 4e-15
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 44 4e-05
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 27 5.3
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 27 9.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 231 bits (565), Expect = 2e-61
Identities = 104/137 (75%), Positives = 120/137 (87%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 303 GDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCH 362
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
TAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 432 AVRDMRQTVAVGVIKAV 482
AVRDMRQTVAVGVIKAV
Sbjct: 423 AVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 231 bits (565), Expect = 2e-61
Identities = 104/137 (75%), Positives = 120/137 (87%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 303 GDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCH 362
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
TAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 432 AVRDMRQTVAVGVIKAV 482
AVRDMRQTVAVGVIKAV
Sbjct: 423 AVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 231 bits (565), Expect = 2e-61
Identities = 104/137 (75%), Positives = 120/137 (87%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 303 GDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCH 362
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
TAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 432 AVRDMRQTVAVGVIKAV 482
AVRDMRQTVAVGVIKAV
Sbjct: 423 AVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 77.8 bits (183), Expect = 4e-15
Identities = 42/125 (33%), Positives = 69/125 (55%), Gaps = 1/125 (0%)
Frame = +3
Query: 114 ELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 293
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K
Sbjct: 540 DVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598
Query: 294 VDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-V 470
+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V
Sbjct: 599 LDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKV 657
Query: 471 IKAVN 485
+K ++
Sbjct: 658 VKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 44.4 bits (100), Expect = 4e-05
Identities = 39/135 (28%), Positives = 60/135 (44%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G V + ++ V +LR G + + +N P R F A++ + G I +G T VL
Sbjct: 464 GDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL--- 519
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
H+ + K+ K + + S K I L PLC+ +E P LGRF
Sbjct: 520 --HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRF 574
Query: 432 AVRDMRQTVAVGVIK 476
+R TVA G++K
Sbjct: 575 ILRRSGDTVAAGIVK 589
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 27.5 bits (58), Expect = 5.3
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -2
Query: 361 MAASPDLMDLGLSSVDLPVRRSTFS 287
++ +PDL D+ LSSVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.6 bits (56), Expect = 9.3
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = +1
Query: 334 NPLNLVMPPLSTWFPPSPCVWS---PSRNSHPSVVSPCVT*GRRSPSV**RL*TSRKQVV 504
+PL+ + P L WF V+S P+R+S + P + + SP+ +++V+
Sbjct: 707 SPLSELHPQLREWFSSKQSVYSLQYPTRDSIIAFFQPILELIKASPTELPGGIPRKRRVL 766
Query: 505 AKSPKLPKKPP 537
+ P P PP
Sbjct: 767 PELPLAPDPPP 777
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,189,021
Number of Sequences: 5004
Number of extensions: 80569
Number of successful extensions: 211
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 880085256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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