Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_G03_e407_13.seq
(1562 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 214 1e-55
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 214 1e-55
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 214 1e-55
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 214 1e-55
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 141 1e-33
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 71 2e-12
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 52 1e-06
At4g27900.2 68417.m04005 expressed protein 32 1.2
At4g27900.1 68417.m04004 expressed protein 32 1.2
At1g45160.1 68414.m05177 protein kinase family protein contains ... 31 2.7
At1g28280.1 68414.m03471 VQ motif-containing protein contains PF... 29 6.3
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 214 bits (523), Expect = 1e-55
Identities = 94/138 (68%), Positives = 119/138 (86%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G VGFNVKNV+VK+L+RGYVA +SK++P +GAA+FT+QVI++NHPGQI NGY PVLDCH
Sbjct: 293 GDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCH 352
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
T+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRF
Sbjct: 353 TSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRF 412
Query: 432 AVRDMRQTVAVGVIKAVN 485
AVRDMRQTVAVGVIK+V+
Sbjct: 413 AVRDMRQTVAVGVIKSVD 430
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 214 bits (523), Expect = 1e-55
Identities = 94/138 (68%), Positives = 119/138 (86%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G VGFNVKNV+VK+L+RGYVA +SK++P +GAA+FT+QVI++NHPGQI NGY PVLDCH
Sbjct: 293 GDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCH 352
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
T+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRF
Sbjct: 353 TSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRF 412
Query: 432 AVRDMRQTVAVGVIKAVN 485
AVRDMRQTVAVGVIK+V+
Sbjct: 413 AVRDMRQTVAVGVIKSVD 430
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 214 bits (523), Expect = 1e-55
Identities = 94/138 (68%), Positives = 119/138 (86%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G VGFNVKNV+VK+L+RGYVA +SK++P +GAA+FT+QVI++NHPGQI NGY PVLDCH
Sbjct: 293 GDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCH 352
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
T+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRF
Sbjct: 353 TSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRF 412
Query: 432 AVRDMRQTVAVGVIKAVN 485
AVRDMRQTVAVGVIK+V+
Sbjct: 413 AVRDMRQTVAVGVIKSVD 430
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 214 bits (523), Expect = 1e-55
Identities = 94/138 (68%), Positives = 119/138 (86%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G VGFNVKNV+VK+L+RGYVA +SK++P +GAA+FT+QVI++NHPGQI NGY PVLDCH
Sbjct: 293 GDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCH 352
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
T+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRF
Sbjct: 353 TSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRF 412
Query: 432 AVRDMRQTVAVGVIKAVN 485
AVRDMRQTVAVGVIK+V+
Sbjct: 413 AVRDMRQTVAVGVIKSVD 430
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 141 bits (342), Expect = 1e-33
Identities = 61/95 (64%), Positives = 77/95 (81%)
Frame = +3
Query: 198 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVP 377
+NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTP 60
Query: 378 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 482
+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V
Sbjct: 61 TKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 71.3 bits (167), Expect = 2e-12
Identities = 37/136 (27%), Positives = 67/136 (49%), Gaps = 2/136 (1%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLN--HPGQISNGYTPVLD 245
G + + + +++ G+V S NP +F AQ+ +L + GY +L
Sbjct: 388 GENLRVRITGIEDEDILSGFVLS-SIVNPVPAVTEFVAQLQILELLDNAIFTAGYKAILH 446
Query: 246 CHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLG 425
H C+ E+K ++D +T K + +K+G A + + + +C+E F +FP LG
Sbjct: 447 IHAVVEECEIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLG 506
Query: 426 RFAVRDMRQTVAVGVI 473
RF +R +T+AVG +
Sbjct: 507 RFTLRTEGKTIAVGKV 522
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 52.0 bits (119), Expect = 1e-06
Identities = 34/137 (24%), Positives = 62/137 (45%)
Frame = +3
Query: 72 GTRVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 251
G V ++ + ++ G V + P A V+VL I G H
Sbjct: 528 GDNVALALQGIDANQVMAGDVLCHP-DFPVSVATHLELMVLVLEGATPILLGSQLEFHVH 586
Query: 252 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 431
A A ++ +D +TG+ T+ +P+ + + +A++ + P+CVE+F E LGR
Sbjct: 587 HAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGRV 646
Query: 432 AVRDMRQTVAVGVIKAV 482
+R +TVA+G + +
Sbjct: 647 FLRSSGRTVAMGKVTRI 663
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 1.2
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +3
Query: 330 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 422
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 1.2
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +3
Query: 330 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 422
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At1g45160.1 68414.m05177 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 1067
Score = 30.7 bits (66), Expect = 2.7
Identities = 21/67 (31%), Positives = 28/67 (41%), Gaps = 1/67 (1%)
Frame = +3
Query: 228 YTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESF- 404
Y P CH AC E++ R S +D K IK + N SK + ++SF
Sbjct: 82 YKPKYSCHPPKYACSSTTSSEEIKLRGKNSGKDEEKMIKISE---TNPPCSKSMGIKSFS 138
Query: 405 QEFPPLG 425
E P G
Sbjct: 139 HELGPRG 145
>At1g28280.1 68414.m03471 VQ motif-containing protein contains
PF05678: VQ motif
Length = 247
Score = 29.5 bits (63), Expect = 6.3
Identities = 17/39 (43%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +1
Query: 331 LNPLNLVMPPLSTWFPP-SPCVWSPSRNSHPSVVSPCVT 444
+NPLN V P+++ F P P + SPS PS+V VT
Sbjct: 140 INPLNPVFNPVNSAFSPRKPEILSPSILDFPSLVLSPVT 178
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,574,585
Number of Sequences: 28952
Number of extensions: 448419
Number of successful extensions: 1278
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1275
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4202426112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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