BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030905E5_F08_e446_12.seq (1590 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74470.1 68414.m08627 geranylgeranyl reductase identical to g... 38 0.024 At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R6... 33 0.52 At1g50970.1 68414.m05730 membrane trafficking VPS53 family prote... 31 2.1 >At1g74470.1 68414.m08627 geranylgeranyl reductase identical to geranylgeranyl reductase GB:Y14044 [Arabidopsis thaliana] (involvement: chlorophyll, the tocopherol and the phylloquinone pathways Eur J Biochem 1998 Jan 15;251(1-2):413-7) Length = 467 Score = 37.5 bits (83), Expect = 0.024 Identities = 18/42 (42%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = +3 Query: 567 DKEDALKNLIQLLKKDD-SPVNYGYVFALCEHMGCGTWTVTH 689 +K ++L ++ DD SP YG+VF C+H+ GT TVTH Sbjct: 242 EKMTYYEDLAEMYVGDDVSPDFYGWVFPKCDHVAVGTGTVTH 283 >At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R65511, gb|T42324 and gb|T20569 come from this gene Length = 571 Score = 33.1 bits (72), Expect = 0.52 Identities = 25/96 (26%), Positives = 47/96 (48%) Frame = +3 Query: 204 AISNANILAGTIELKRLQSILEDSLKSKDISSLYYAVKGLKLLKAPIPDICEDIKTIKYD 383 A AN+ E K LQ E++ K +D L + + L+ I IC+D+K +K Sbjct: 431 AEQEANMEKVVQESKLLQQEAEENSKLRDF--LMDRGQIVDTLQGEISVICQDVKLLKEK 488 Query: 384 VKNIEQIFQLTNAAALTDCVNFLKPEVLSTPAQVLD 491 +N + + +++ + C + +K VL P++ L+ Sbjct: 489 FENRVPLTKSISSSFTSSCGSSMKSLVLENPSERLN 524 >At1g50970.1 68414.m05730 membrane trafficking VPS53 family protein contains Pfam domain PF04100: Vps53-like, N-terminal Length = 569 Score = 31.1 bits (67), Expect = 2.1 Identities = 30/123 (24%), Positives = 57/123 (46%), Gaps = 3/123 (2%) Frame = +3 Query: 249 RLQSILEDSL-KSKDISSLYYAVKG-LKLLKAPIPDICEDIKTIKYDVKNIEQIFQLTNA 422 R + L D++ ++++S +K + +A + DIC DIK + + KNI +T Sbjct: 54 RAKENLNDAICAAEELSHKIQEIKSKAEQTEAMVQDICSDIKKLDFAKKNITT--AVTAL 111 Query: 423 AALTDCVNFLKPEVLSTPAQVLDKKDATLSEIYYSVYALKA-LGKGSVFDKEDALKNLIQ 599 + LT V+ ++ + T + + L I KA + + + + LKN+ Q Sbjct: 112 SRLTMLVSAVQQLQVMTSKRQYKEAATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQ 171 Query: 600 LLK 608 +LK Sbjct: 172 ILK 174 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 25,247,743 Number of Sequences: 28952 Number of extensions: 482429 Number of successful extensions: 1936 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 1170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1815 length of database: 12,070,560 effective HSP length: 84 effective length of database: 9,638,592 effective search space used: 4289173440 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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