Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_F04_e414_12.seq
(1648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 108 1e-23
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 108 1e-23
At2g27090.1 68415.m03255 expressed protein contains Pfam domains... 31 2.2
At2g17200.1 68415.m01986 ubiquitin family protein weak similarit... 30 5.0
At2g17190.1 68415.m01985 ubiquitin family protein contains INTER... 30 5.0
At3g18830.1 68416.m02391 mannitol transporter, putative similar ... 29 6.7
At3g56990.1 68416.m06344 glycine-rich protein conserved hypothet... 29 8.8
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 108 bits (259), Expect = 1e-23
Identities = 49/130 (37%), Positives = 75/130 (57%), Gaps = 3/130 (2%)
Frame = +3
Query: 177 SKDPFDSMPKGSFNMDDFKRFYSNEEE---AKSIPYFWEKFDPEHYSIWYCQYKYPEELA 347
+K+P D +P +DD+KR YSN + +I FW+ +DPE YS+W+C YKY +E
Sbjct: 253 AKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENM 312
Query: 348 KVFMSCNLITGMFQRLDKMRKQAFASVCLFGGDNDSTISGVWVWRGHELAFPLSPDWQID 527
F++ N + G QR+D RK +F + + G + + G+W++RG E+ D D
Sbjct: 313 VSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYD 371
Query: 528 YESYEWTKLD 557
E YEWTK+D
Sbjct: 372 MELYEWTKVD 381
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 108 bits (259), Expect = 1e-23
Identities = 49/130 (37%), Positives = 75/130 (57%), Gaps = 3/130 (2%)
Frame = +3
Query: 177 SKDPFDSMPKGSFNMDDFKRFYSNEEE---AKSIPYFWEKFDPEHYSIWYCQYKYPEELA 347
+K+P D +P +DD+KR YSN + +I FW+ +DPE YS+W+C YKY +E
Sbjct: 254 AKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENM 313
Query: 348 KVFMSCNLITGMFQRLDKMRKQAFASVCLFGGDNDSTISGVWVWRGHELAFPLSPDWQID 527
F++ N + G QR+D RK +F + + G + + G+W++RG E+ D D
Sbjct: 314 VSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYD 372
Query: 528 YESYEWTKLD 557
E YEWTK+D
Sbjct: 373 MELYEWTKVD 382
>At2g27090.1 68415.m03255 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 743
Score = 31.1 bits (67), Expect = 2.2
Identities = 23/96 (23%), Positives = 43/96 (44%), Gaps = 1/96 (1%)
Frame = +3
Query: 195 SMPKGSFNMDDFKRFYSNEEEAKSIPYFWEKFDPEHYSIWYCQYKYPEELAKVFMSCNLI 374
S GS FK S E+ K +P +P S+ Y + + E ++ S N +
Sbjct: 347 SKESGSGASSLFKTCLSCGEDPKDVPE-----EPAQNSVKYLTW-HRTESSRSSSSRNPL 400
Query: 375 TGM-FQRLDKMRKQAFASVCLFGGDNDSTISGVWVW 479
GM ++++ F ++C+ G + ST+ ++ W
Sbjct: 401 GGMNSDDVEELNSNLFENICMIAGSHASTLDRLYAW 436
>At2g17200.1 68415.m01986 ubiquitin family protein weak similarity
to PLIC-2 (ubiquitin-like type II) [Homo sapiens]
GI:9937505; contains Pfam profiles PF00240: Ubiquitin
family, PF00627: UBA/TS-N domain
Length = 551
Score = 29.9 bits (64), Expect = 5.0
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +3
Query: 123 KSLQNPL*RKSLMRNPRXSKDPFDSMPKGSFNMDDFKRFYSNEEE 257
++ +NP + +MRN + +SMP+G FNM +R Y N +E
Sbjct: 225 EAARNPELMREMMRNTDRAMSNIESMPEG-FNM--LRRMYENVQE 266
>At2g17190.1 68415.m01985 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 538
Score = 29.9 bits (64), Expect = 5.0
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +3
Query: 123 KSLQNPL*RKSLMRNPRXSKDPFDSMPKGSFNMDDFKRFYSNEEE 257
++ +NP + +MRN + +SMP+G FNM +R Y N +E
Sbjct: 220 EAARNPELMREMMRNTDRAMSNIESMPEG-FNM--LRRMYENVQE 261
>At3g18830.1 68416.m02391 mannitol transporter, putative similar to
mannitol transporter [Apium graveolens var. dulce]
GI:12004316; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 539
Score = 29.5 bits (63), Expect = 6.7
Identities = 16/38 (42%), Positives = 18/38 (47%)
Frame = -1
Query: 508 ESGKASSWPRQTHTPEMVESLSPPKRQTEAKACLRILS 395
E+ A S P H PE V PPKR A AC + S
Sbjct: 7 ENRTAPSPPPVKHVPESVLPAKPPKRNNYAFACAILAS 44
>At3g56990.1 68416.m06344 glycine-rich protein conserved
hypothetical protein SPCC330.09 - Schizosaccharomyces
pombe, PIR:T41319
Length = 711
Score = 29.1 bits (62), Expect = 8.8
Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = +3
Query: 507 SPDWQIDYESYEWTKLDPHSPDTK--ALLRDYFSWTGTDKD 623
+PD+QID +SYE+ L P + K +LL ++F D +
Sbjct: 510 NPDFQIDKDSYEYGVLHPVASSKKQPSLLDEHFEAVSDDDE 550
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,933,324
Number of Sequences: 28952
Number of extensions: 392368
Number of successful extensions: 932
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 905
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 927
length of database: 12,070,560
effective HSP length: 85
effective length of database: 9,609,640
effective search space used: 4449263320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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