BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030905E5_F03_e406_11.seq (1575 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch... 62 2e-10 SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|ch... 56 2e-08 SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr... 42 3e-04 SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 39 0.002 SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 37 0.009 SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 36 0.020 SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 34 0.047 SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr... 32 0.19 SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces po... 28 4.1 SPBPB8B6.04c |grt1|SPAPB8B6.04c, SPAPB8B6.04c|transcription fact... 27 7.1 SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomy... 27 9.4 SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 27 9.4 >SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 62.1 bits (144), Expect = 2e-10 Identities = 36/140 (25%), Positives = 65/140 (46%), Gaps = 1/140 (0%) Frame = +2 Query: 332 RVVVLGSPRSGKSAVVVRYLTKRYIGEYSSTGDFLYQHRVAFDGAVSEVEILDTSNCAIR 511 ++VV+G GKSA+ ++ + ++ EY T + Y+ + DG + +++LDT+ Sbjct: 10 KLVVVGDGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKKCEIDGEGALLDVLDTAGQEEY 69 Query: 512 GCLAE-HVRWGDAFAVVYSVCXXXXXXXXXXXXXXXXXTRLPGCAAVTLLGNKRDLEHAR 688 + E ++R G+ F +VY++ + V L+ NK DLE R Sbjct: 70 SAMREQYMRTGEGFLLVYNITSRSSFDEISTFYQQILRVKDKDTFPVVLVANKCDLEAER 129 Query: 689 EVHAEEGQXLSLRFGCQFYE 748 V EG+ L+ C + E Sbjct: 130 VVSRAEGEQLAKSMHCLYVE 149 >SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|chr 2|||Manual Length = 185 Score = 55.6 bits (128), Expect = 2e-08 Identities = 36/140 (25%), Positives = 61/140 (43%), Gaps = 1/140 (0%) Frame = +2 Query: 332 RVVVLGSPRSGKSAVVVRYLTKRYIGEYSSTGDFLYQHRVAFDGAVSEVEILDTSNCAIR 511 R+ VLGS GKS++ V+Y+ ++ Y T + + + + G EI+DT+ Sbjct: 8 RIAVLGSRSVGKSSLTVQYVENHFVESYYPTIENTFSKNIKYKGQEFATEIIDTAGQDEY 67 Query: 512 GCL-AEHVRWGDAFAVVYSVCXXXXXXXXXXXXXXXXXTRLPGCAAVTLLGNKRDLEHAR 688 L ++H + +VYS+ + ++GNK DL R Sbjct: 68 SILNSKHSIGIHGYVLVYSITSKSSFEMVKIVRDKILNHTGTEWVPIVVVGNKSDLHMQR 127 Query: 689 EVHAEEGQXLSLRFGCQFYE 748 V AEEG+ L+ + C + E Sbjct: 128 AVTAEEGKALANEWKCAWTE 147 >SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 201 Score = 41.5 bits (93), Expect = 3e-04 Identities = 32/135 (23%), Positives = 60/135 (44%), Gaps = 3/135 (2%) Frame = +2 Query: 317 TVNRIRVVVLGSPRSGKSAVVVRYLTKRYIGEYSST-G-DFLYQHRVAFDGAVSEVEILD 490 ++ + ++V LG GK++++ R++ ++ Y +T G DFL + D V +++ D Sbjct: 8 SLRKFKLVFLGEQSVGKTSLITRFMYDQFDNTYQATIGIDFLSKTMYLEDRTV-RLQLWD 66 Query: 491 TSNCA-IRGCLAEHVRWGDAFAVVYSVCXXXXXXXXXXXXXXXXXTRLPGCAAVTLLGNK 667 T+ R + ++R +VY + R + L+GNK Sbjct: 67 TAGQERFRSLIPSYIRDSSVAIIVYDITNHNSFVNTEKWIEDVRAERGDD-VIIVLVGNK 125 Query: 668 RDLEHAREVHAEEGQ 712 DL R+V EEG+ Sbjct: 126 TDLADKRQVTQEEGE 140 >SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 39.1 bits (87), Expect = 0.002 Identities = 32/142 (22%), Positives = 60/142 (42%), Gaps = 2/142 (1%) Frame = +2 Query: 329 IRVVVLGSPRSGKSAVVVRYLTKRYIGEYSSTGDFLYQHR-VAFDGAVSEVEILDTSNCA 505 I+++++G GKS +++R+ + + +T ++ R + DG +++I DT+ Sbjct: 10 IKLLLIGDSGVGKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQE 69 Query: 506 -IRGCLAEHVRWGDAFAVVYSVCXXXXXXXXXXXXXXXXXTRLPGCAAVTLLGNKRDLEH 682 R + R ++Y V + L+GNK D E Sbjct: 70 RFRTITTAYYRGAMGILLLYDVTDKKSFDNVRTWFSNVEQHASENVYKI-LIGNKCDCED 128 Query: 683 AREVHAEEGQXLSLRFGCQFYE 748 R+V E+GQ L+ G +F E Sbjct: 129 QRQVSFEQGQALADELGVKFLE 150 >SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1|||Manual Length = 214 Score = 36.7 bits (81), Expect = 0.009 Identities = 32/141 (22%), Positives = 51/141 (36%), Gaps = 2/141 (1%) Frame = +2 Query: 332 RVVVLGSPRSGKSAVVVRYLTKRYIGEYSST-GDFLYQHRVAFDGAVSEVEILDTSNCA- 505 + V++G GKS +++R+ + E ST G + D + +I DT+ Sbjct: 12 KTVLIGDSGVGKSNLLMRFTRNEFNIESKSTIGVEFATRNIVLDNKKIKAQIWDTAGQER 71 Query: 506 IRGCLAEHVRWGDAFAVVYSVCXXXXXXXXXXXXXXXXXTRLPGCAAVTLLGNKRDLEHA 685 R + + R +VY + + L+GNK DL H Sbjct: 72 YRAITSAYYRGAVGALIVYDI-TKQSSFDNVGRWLKELREHADSNIVIMLVGNKTDLLHL 130 Query: 686 REVHAEEGQXLSLRFGCQFYE 748 R V EE Q + F E Sbjct: 131 RAVSTEEAQAFAAENNLSFIE 151 >SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 35.5 bits (78), Expect = 0.020 Identities = 29/131 (22%), Positives = 55/131 (41%), Gaps = 5/131 (3%) Frame = +2 Query: 329 IRVVVLGSPRSGKSAVVVRYLTKRYIGEYSSTGDFLYQHRVAFDGAVSE-----VEILDT 493 +++V+ G +GKS ++ R++ ++ + S T + R+ G ++ ++I DT Sbjct: 10 VKIVLAGPSGTGKSCLLQRFVKNQWDDQVSHTVGIDFASRIISVGMGNQQKRIKLQIWDT 69 Query: 494 SNCAIRGCLAEHVRWGDAFAVVYSVCXXXXXXXXXXXXXXXXXTRLPGCAAVTLLGNKRD 673 + +A + G A AV+ P V L G+K D Sbjct: 70 AGQEKFRSVARNYYRGAAGAVLVYDVTNKDSFEELSSWLSDIRAMAPSTICVVLAGSKSD 129 Query: 674 LEHAREVHAEE 706 L++ R+V EE Sbjct: 130 LQNQRQVSTEE 140 >SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2|||Manual Length = 205 Score = 34.3 bits (75), Expect = 0.047 Identities = 29/128 (22%), Positives = 54/128 (42%), Gaps = 6/128 (4%) Frame = +2 Query: 329 IRVVVLGSPRSGKSAVVVRYLTKRYIGEYSST--GDFLYQHRVAFDGAVSEVEILDTSNC 502 ++V++LG GK++++ +Y+ +++ +Y +T DFL + V D V +++ DT+ Sbjct: 9 LKVIILGESGVGKTSIMNQYVNRKFSKDYKATIGADFLTK-EVLVDDKVVTLQLWDTAGQ 67 Query: 503 -AIRGCLAEHVRWGDAFAVVYSVCXXXXXXXXXX---XXXXXXXTRLPGCAAVTLLGNKR 670 + R D +VY V P LLGNK Sbjct: 68 ERFQSLGVAFYRGADCCVLVYDVNNSKSFETLDSWRDEFLIQASPSNPETFPFILLGNKV 127 Query: 671 DLEHAREV 694 D+E + + Sbjct: 128 DVEEQKRM 135 >SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr 1|||Manual Length = 208 Score = 32.3 bits (70), Expect = 0.19 Identities = 22/83 (26%), Positives = 44/83 (53%), Gaps = 3/83 (3%) Frame = +2 Query: 329 IRVVVLGSPRSGKSAVVVRYLTKRYIGEYSST--GDFLYQHRVAFDGAVSEVEILDTSNC 502 ++VV+LG GK+ ++ +++ +++ EY +T DFL + V D V+ +++ DT+ Sbjct: 9 LKVVILGDSGVGKTCLMNQFVNQKFSREYKATIGADFLTKDVVVDDKLVT-LQLWDTAGQ 67 Query: 503 -AIRGCLAEHVRWGDAFAVVYSV 568 + R D +VY+V Sbjct: 68 ERFQSLGMAFYRGADCCVIVYNV 90 >SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces pombe|chr 1|||Manual Length = 205 Score = 27.9 bits (59), Expect = 4.1 Identities = 17/56 (30%), Positives = 30/56 (53%), Gaps = 1/56 (1%) Frame = +2 Query: 332 RVVVLGSPRSGKSAVVVRYLTKRYIGE-YSSTGDFLYQHRVAFDGAVSEVEILDTS 496 ++V+LG +GK++ ++ TK Y + Y T Y H + DG E+ + DT+ Sbjct: 15 KIVILGDGAAGKTS-LLNVFTKGYFPQVYEPTIFENYIHDIFVDGNSIELSLWDTA 69 >SPBPB8B6.04c |grt1|SPAPB8B6.04c, SPAPB8B6.04c|transcription factor Grt1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 648 Score = 27.1 bits (57), Expect = 7.1 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +3 Query: 795 HYLREARAXGTYYCPRXDESLLPTLSLRGLERFSGK 902 HYLR+A G + + P +GLE+F K Sbjct: 104 HYLRKAERNGYDFLSEGQNDITPEEERKGLEKFGMK 139 >SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomyces pombe|chr 2|||Manual Length = 251 Score = 26.6 bits (56), Expect = 9.4 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +3 Query: 804 REARAXGTYYCPRXDESLLPTLSLRGLERFSGKTAK 911 R+ARA G YY P + L+ + +RG+ K K Sbjct: 75 RKARAEGNYYVP-DETKLVFVIRIRGINNIPPKARK 109 >SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1513 Score = 26.6 bits (56), Expect = 9.4 Identities = 9/15 (60%), Positives = 11/15 (73%) Frame = +1 Query: 775 RSTCVSCTICERRVH 819 R + + CTICERR H Sbjct: 1364 RDSFIDCTICERRFH 1378 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,060,548 Number of Sequences: 5004 Number of extensions: 69275 Number of successful extensions: 184 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 182 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 888013952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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