Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_E10_e461_10.seq
(1519 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot... 301 1e-81
At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ... 271 7e-73
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S... 230 2e-60
At5g46730.1 68418.m05757 glycine-rich protein 33 0.37
At4g01985.1 68417.m00265 expressed protein 33 0.37
At4g15420.1 68417.m02359 PRLI-interacting factor K nearly identi... 31 1.5
At3g16460.2 68416.m02097 jacalin lectin family protein contains ... 30 3.5
At3g16460.1 68416.m02098 jacalin lectin family protein contains ... 30 3.5
At3g11030.1 68416.m01331 expressed protein contains Pfam domain ... 30 4.6
At5g52170.1 68418.m06476 homeobox-leucine zipper family protein ... 29 6.1
At5g23150.1 68418.m02707 PWWP domain-containing protein identica... 29 6.1
>At1g74030.1 68414.m08573 enolase, putative similar to
Swiss-Prot:P15007 enolase (EC 4.2.1.11)
(2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
hydro-lyase) [Drosophila melanogaster]
Length = 477
Score = 301 bits (738), Expect = 1e-81
Identities = 163/319 (51%), Positives = 204/319 (63%), Gaps = 2/319 (0%)
Frame = +2
Query: 53 PGCRXFGTRXFLKVIHLVSNMPIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGAS 232
P C F R ++ + +K +KARQI DSRGNPTVEVDL+T+ L+R+AVPSGAS
Sbjct: 30 PSCLRF-RRSGVQCSVVAKECRVKGVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGAS 87
Query: 233 TGVHEALELRDNIKSEYHGKGVLTAIKNINDIIAPELLSKNIEVTEQREIDQLMLKLDGT 412
TG++EALELRD KS Y GKGVL AIKNIN+++AP+L+ ++V Q ++D LML+LDGT
Sbjct: 88 TGIYEALELRDGDKSVYGGKGVLQAIKNINELVAPKLIG--VDVRNQADVDALMLELDGT 145
Query: 413 ENKSKLGANAILGVSLXXXXXXXXXXDVPLYKHLADLSGNKDIVLPVPAFNVINGGSHAG 592
NKSKLGANAILGVSL VPLYKH+ + SG K++V+PVPAFNVINGGSHAG
Sbjct: 146 PNKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVINGGSHAG 205
Query: 593 NKLAMQEFMILPTGASSFSEAMRMGSEVYHYLKKXIKEKFGLDSTAVGDEGGFAPTSXTT 772
N LAMQEFMILP GA+SFSEA +MGSEVYH LK IK K+G D+ VGDEGGFAP
Sbjct: 206 NSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDN 265
Query: 773 RKLSISFRMLSXRLDXLAKFALAWMXLLLNSF--KMGPMTLTSXILTXTPXNICLLKKLT 946
R+ + + K + M + + F K G L ++ + L
Sbjct: 266 REGLVLLIDAIEKAGYTGKIKIG-MDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLA 324
Query: 947 XXXLGXXKDFPMGXLXXPF 1003
KDFP+ + PF
Sbjct: 325 DLYREFIKDFPIVSIEDPF 343
>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
enolase (EC 4.2.1.11) (2-phosphoglycerate
dehydratase)(2-phospho-D- glycerate hydro-lyase)
[Arabidopsis thaliana]
Length = 444
Score = 271 bits (665), Expect = 7e-73
Identities = 142/220 (64%), Positives = 167/220 (75%), Gaps = 7/220 (3%)
Frame = +2
Query: 119 IKSIKARQIFDSRGNPTVEVDLVTELGL-FRAAVPSGASTGVHEALELRDNIKSEYHGKG 295
I +KARQIFDSRGNPTVEVD+ T G+ AAVPSGASTG++EALELRD S+Y GKG
Sbjct: 4 ITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDG-GSDYLGKG 62
Query: 296 VLTAIKNINDIIAPELLSKNIEVTEQREIDQLML-KLDGTEN-----KSKLGANAILGVS 457
V A+ N+N+II P L+ K + T+Q ID M+ +LDGT+N K KLGANAIL VS
Sbjct: 63 VSKAVGNVNNIIGPALIGK--DPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 120
Query: 458 LXXXXXXXXXXDVPLYKHLADLSGNKDIVLPVPAFNVINGGSHAGNKLAMQEFMILPTGA 637
L +PLYKH+A+L+GN IVLPVPAFNVINGGSHAGNKLAMQEFMILP GA
Sbjct: 121 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
Query: 638 SSFSEAMRMGSEVYHYLKKXIKEKFGLDSTAVGDEGGFAP 757
+SF EAM+MG EVYH+LK IK+K+G D+T VGDEGGFAP
Sbjct: 181 ASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAP 220
Score = 62.9 bits (146), Expect = 5e-10
Identities = 31/56 (55%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
Frame = +1
Query: 742 GWFCSNIQXNKEALYLIQDAIXQAGXXGKICIGMDXAASEFF-QDGAYDLDFXNPN 906
G F NIQ NKE L L++ AI +AG GK+ IGMD AASEF+ +D YDL+F N
Sbjct: 216 GGFAPNIQENKEGLELLKTAIEKAGYTGKVVIGMDVAASEFYSEDKTYDLNFKEEN 271
>At2g29560.1 68415.m03590 enolase, putative similar to enolase
[Spinacia oleracea] gi|8919731|emb|CAB96173
Length = 475
Score = 230 bits (563), Expect = 2e-60
Identities = 118/213 (55%), Positives = 147/213 (69%)
Frame = +2
Query: 119 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGV 298
I +KARQI DSRG PTVEVDL T G+FRA+VPSG S+G +EA+ELRD K Y G V
Sbjct: 47 ITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSV 106
Query: 299 LTAIKNINDIIAPELLSKNIEVTEQREIDQLMLKLDGTENKSKLGANAILGVSLXXXXXX 478
A+KNIN+ I+ L+ + ++ Q +IDQ M+ LD TE KS+LGANAIL VS+
Sbjct: 107 AKAVKNINEKISEALIGMDPKL--QGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAG 164
Query: 479 XXXXDVPLYKHLADLSGNKDIVLPVPAFNVINGGSHAGNKLAMQEFMILPTGASSFSEAM 658
+VPL KHL+DLSG ++VLPVPAF V++GG HA N A+QE MILP GAS F EA+
Sbjct: 165 AAEKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEAL 224
Query: 659 RMGSEVYHYLKKXIKEKFGLDSTAVGDEGGFAP 757
+ GSE YH+LK I EK G VG++GG AP
Sbjct: 225 QWGSETYHHLKAVISEKNGGLGCNVGEDGGLAP 257
Score = 43.2 bits (97), Expect = 5e-04
Identities = 22/55 (40%), Positives = 30/55 (54%)
Frame = +1
Query: 742 GWFCSNIQXNKEALYLIQDAIXQAGXXGKICIGMDXAASEFFQDGAYDLDFXNPN 906
G +I KE L L+++AI + G KI I +D AA+ F YDLD +PN
Sbjct: 253 GGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYDLDIKSPN 307
>At5g46730.1 68418.m05757 glycine-rich protein
Length = 290
Score = 33.5 bits (73), Expect = 0.37
Identities = 27/129 (20%), Positives = 28/129 (21%)
Frame = -2
Query: 1452 GXXXXGGGXXXXXKXXXXXXXXGXXXXXGGXXXXXXXXXXXXXGXGXXXXXXWGXXGXXX 1273
G GGG G G G G +G G
Sbjct: 83 GGSGHGGGGGGAASSGGYASGAGEGGGGGYGGAAGGHAGGGGGGSGGGGGSAYGAGGEHA 142
Query: 1272 XXXXGXGXGXGXXGXXXXXXXGXGXXGXXGXXXXGRXXXXXXXXXXGXXAXXXXXFXXGG 1093
G G G G G G G G G G G GG
Sbjct: 143 S---GYGNGAGEGGGAGASGYGGGAYGGGGGHGGGGGGGSAGGAHGGSGYGGGEGGGAGG 199
Query: 1092 GXVRXGXGG 1066
G G GG
Sbjct: 200 GGSHGGAGG 208
Score = 30.7 bits (66), Expect = 2.6
Identities = 25/131 (19%), Positives = 25/131 (19%)
Frame = -2
Query: 1458 GXGXXXXGGGXXXXXKXXXXXXXXGXXXXXGGXXXXXXXXXXXXXGXGXXXXXXWGXXGX 1279
G G GGG G G G G G
Sbjct: 84 GSGHGGGGGGAASSGGYASGAGEGGGGGYGGAAGGHAGGGGGGSGGGGGSAYGAGGEHAS 143
Query: 1278 XXXXXXGXGXGXGXXGXXXXXXXGXGXXGXXGXXXXGRXXXXXXXXXXGXXAXXXXXFXX 1099
G G G G G G G G G G
Sbjct: 144 GYGNGAGEGGGAGASGYGGGAYGGGGGHGGGGGGGSAGGAHGGSGYGGGEGGGAGGGGSH 203
Query: 1098 GGGXVRXGXGG 1066
GG G GG
Sbjct: 204 GGAGGYGGGGG 214
>At4g01985.1 68417.m00265 expressed protein
Length = 579
Score = 33.5 bits (73), Expect = 0.37
Identities = 31/143 (21%), Positives = 32/143 (22%)
Frame = -2
Query: 1494 GXXXXVXGXRXXGXGXXXXGGGXXXXXKXXXXXXXXGXXXXXGGXXXXXXXXXXXXXGXG 1315
G G G G GGG GG G G
Sbjct: 360 GAVGGAVGGAVGGGGGGSVGGGGRGSGGASGGASGGASGGASGGASGGASGGVGGAGGAG 419
Query: 1314 XXXXXXWGXXGXXXXXXXGXGXGXGXXGXXXXXXXGXGXXGXXGXXXXGRXXXXXXXXXX 1135
G G G G G G G G G G G GR
Sbjct: 420 GSVGAG-GGVGGGVGGGVGGGVG-GAVGGAVGGAVGGGGGGSVGGG--GRGSGGAGGGTG 475
Query: 1134 GXXAXXXXXFXXGGGXVRXGXGG 1066
G GGG + G GG
Sbjct: 476 GSVGAGGGVGVGGGGGIGGGAGG 498
>At4g15420.1 68417.m02359 PRLI-interacting factor K nearly identical
to PRLI-interacting factor K [Arabidopsis thaliana]
GI:11139266; contains Pfam profiles PF03152: Ubiquitin
fusion degradation protein UFD1, PF00096: Zinc finger,
C2H2 type
Length = 561
Score = 31.5 bits (68), Expect = 1.5
Identities = 23/96 (23%), Positives = 47/96 (48%)
Frame = +2
Query: 92 VIHLVSNMPIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNI 271
V + VS + +SI ++ + + + +VD V E + ++PS S +HE R N+
Sbjct: 370 VKYQVSVLVQESIDGAKVGERAVSSSSDVDTV-ECRNCKHSIPS-RSIALHEVYCSRHNV 427
Query: 272 KSEYHGKGVLTAIKNINDIIAPELLSKNIEVTEQRE 379
+HG G++ ++ + + E K ++ TE +
Sbjct: 428 VCNHHGCGIVLRVEEAKNHLHCEKCGKALQPTEMEK 463
>At3g16460.2 68416.m02097 jacalin lectin family protein contains Pfam
profile: PF01419 jacalin-like lectin domain; similar to
myrosinase binding protein [Brassica napus] GI:1711296,
GI:1655824, myrosinase-binding protein homolog
[Arabidopsis thaliana] GI:2997767; contains Pfam profile
PF01419 jacalin-like lectin family
Length = 647
Score = 30.3 bits (65), Expect = 3.5
Identities = 20/86 (23%), Positives = 20/86 (23%)
Frame = -2
Query: 1323 GXGXXXXXXWGXXGXXXXXXXGXGXGXGXXGXXXXXXXGXGXXGXXGXXXXGRXXXXXXX 1144
G G G G G G G G G G G G G G
Sbjct: 139 GTGTGTGTGTGT-GTGTGTGTGTGTGTGTGGTGTGTGTGTGGTG-TGTGTGGTGTGTGTG 196
Query: 1143 XXXGXXAXXXXXFXXGGGXVRXGXGG 1066
G GG G GG
Sbjct: 197 TGTGTGTGTGTGTGTGGTGTGTGTGG 222
>At3g16460.1 68416.m02098 jacalin lectin family protein contains Pfam
profile: PF01419 jacalin-like lectin domain; similar to
myrosinase binding protein [Brassica napus] GI:1711296,
GI:1655824, myrosinase-binding protein homolog
[Arabidopsis thaliana] GI:2997767; contains Pfam profile
PF01419 jacalin-like lectin family
Length = 705
Score = 30.3 bits (65), Expect = 3.5
Identities = 20/86 (23%), Positives = 20/86 (23%)
Frame = -2
Query: 1323 GXGXXXXXXWGXXGXXXXXXXGXGXGXGXXGXXXXXXXGXGXXGXXGXXXXGRXXXXXXX 1144
G G G G G G G G G G G G G G
Sbjct: 139 GTGTGTGTGTGT-GTGTGTGTGTGTGTGTGGTGTGTGTGTGGTG-TGTGTGGTGTGTGTG 196
Query: 1143 XXXGXXAXXXXXFXXGGGXVRXGXGG 1066
G GG G GG
Sbjct: 197 TGTGTGTGTGTGTGTGGTGTGTGTGG 222
>At3g11030.1 68416.m01331 expressed protein contains Pfam domain
PF03005: Arabidopsis proteins of unknown function
Length = 451
Score = 29.9 bits (64), Expect = 4.6
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = +3
Query: 1284 PPXPTXPXXPXPPPXSP 1334
PP PT P P PPP SP
Sbjct: 66 PPPPTSPPPPSPPPPSP 82
>At5g52170.1 68418.m06476 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein similar to
Anthocyaninless2 (ANL2) (GP:5702094) [Arabidopsis
thaliana]; contains Pfam PF00046: Homeobox domain and
Pfam PF01852: START domain
Length = 682
Score = 29.5 bits (63), Expect = 6.1
Identities = 20/48 (41%), Positives = 27/48 (56%), Gaps = 3/48 (6%)
Frame = +2
Query: 518 DLSGNKDIVLPV--PAFN-VINGGSHAGNKLAMQEFMILPTGASSFSE 652
D+SG + PV P+ N V++GG A L F ILP G+SS S+
Sbjct: 574 DVSGALVVYAPVDIPSMNTVMSGGDSAYVALLPSGFSILPDGSSSSSD 621
>At5g23150.1 68418.m02707 PWWP domain-containing protein identical to
cDNA putative transcription factor (HUA2) GI:4868119;
contains Pfam profile PF00855: PWWP domain
Length = 1392
Score = 29.5 bits (63), Expect = 6.1
Identities = 11/23 (47%), Positives = 12/23 (52%)
Frame = +3
Query: 1266 PXSXXXPPXPTXPXXPXPPPXSP 1334
P + PP P P P PPP SP
Sbjct: 1095 PPAALFPPLPPPPSQPPPPPLSP 1117
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,557,491
Number of Sequences: 28952
Number of extensions: 516641
Number of successful extensions: 1858
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1273
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1728
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4057847232
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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