Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_D08_e444_08.seq
(1556 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 187 4e-48
SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces pomb... 38 0.003
SPAC56E4.03 |||aromatic aminotransferase |Schizosaccharomyces po... 33 0.11
SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 31 0.33
SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces pom... 29 1.7
SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces... 29 2.3
SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc... 27 5.3
SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces p... 27 7.0
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 7.0
SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces p... 27 7.0
SPAC26F1.10c |pyp1||tyrosine phosphatase Pyp1|Schizosaccharomyce... 27 9.3
>SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 421
Score = 187 bits (455), Expect = 4e-48
Identities = 104/235 (44%), Positives = 145/235 (61%), Gaps = 4/235 (1%)
Frame = +2
Query: 140 VWVEYIQLAAEYK-PAVNLGQGFPDYHAPEHVTKALADIAVGDNPLLNQYTRGFGHPRLV 316
VW Q AE K P V+L QGF +Y+ P+ V A A ++ D NQY+ G P L
Sbjct: 19 VWTLVNQATAECKVPPVSLSQGFFNYNPPKFVLDA-AKKSI-DEVACNQYSHTRGRPSLR 76
Query: 317 QNLSKVYSPLIGREIDPFNEILVTSGAYEALFSAILGHVDTGDEVIVIEPFFDCYDFMIK 496
+ LS+ YSP R ++P EI+VT+GA E FS ++ GDEVIV+EPFFD Y I
Sbjct: 77 KALSEAYSPYFKRTLNPDTEIVVTAGANEGFFSVFAAFLNPGDEVIVMEPFFDQYISNIT 136
Query: 497 SAGGVPKFIALKPKVSSGT--ITSADWVLDESELVSLFTSKTKMIILNTPHNPLGKAFTR 670
GGVP ++ + P +++ W LD ++L + T KTKMI++NTPHNPLGK F+
Sbjct: 137 MNGGVPVYVPIIPPEEGSVKPVSAGAWKLDMNKLRNAITEKTKMIVINTPHNPLGKIFSE 196
Query: 671 QELELIADLCKKHNVLCLSDEVYEWMVYEPVKHIRIATL-PGMWERTITVGSAGE 832
+EL IADL KHN+L +SDEVY+ + + P +R+ATL P +++ +TVGS G+
Sbjct: 197 EELNEIADLVLKHNLLVVSDEVYDRLSFVP--FVRLATLRPELFKHVVTVGSGGK 249
Score = 33.9 bits (74), Expect = 0.061
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +3
Query: 828 GKTFSVTGWKTRWAYGPAXLMRNLQXGHXNXVYPCCXPVQK 950
GKTF TGW+ W G L++ H + P Q+
Sbjct: 248 GKTFGCTGWRVGWLIGDESLIKYSAAAHTRICFAVNSPCQE 288
>SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 38.3 bits (85), Expect = 0.003
Identities = 39/174 (22%), Positives = 71/174 (40%), Gaps = 9/174 (5%)
Frame = +2
Query: 275 LNQYTRGFGHPRLVQNLSKVYSPLIGREIDPFNEILVTSGAYEA---LFSAILGHVDTGD 445
L Y+ G P + ++++ G + +P ++I +TSGA A + + I+ G
Sbjct: 133 LGAYSASQGIPLVRRHVADFIRARDGFDCEP-SDIYLTSGASHAARLIMTLIIARPTDG- 190
Query: 446 EVIVIEPFFDCYDFMIKSAGGVPKFIALKPKVSSGTITSADWVLDESELVSLFTSKTK-- 619
V+V P + Y I G VS +W +D + F +K
Sbjct: 191 -VMVPAPQYPLYGAQIDLMSG--------SMVSYSLSEENNWDIDFDQFKKSFDEASKKG 241
Query: 620 ----MIILNTPHNPLGKAFTRQELELIADLCKKHNVLCLSDEVYEWMVYEPVKH 769
+ ++ P NP G + +E + K ++ L+DEVY+ +Y+ H
Sbjct: 242 INVRLCVVINPGNPTGACISENSMEKVLRFAKAKGIVLLADEVYQNNIYQNKFH 295
>SPAC56E4.03 |||aromatic aminotransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 33.1 bits (72), Expect = 0.11
Identities = 36/157 (22%), Positives = 67/157 (42%), Gaps = 1/157 (0%)
Frame = +2
Query: 281 QYTRGFGHPRLVQNLSKVYSPLIGREIDPFNEILVTSGAYEALFSAILGHVDTGDEVIVI 460
QY +G G L Q L + + + +N I++T+G L A+ + GD ++V
Sbjct: 98 QYGQGSGAALLSQFLKEHTRIIHNPPYEGWN-IIMTTGNTSCLDIALRMLTNRGDSILVE 156
Query: 461 EPFFDCYDFMIKSAGGVPKFIALKPKVSSGTITSADWVLDESELVSLFTSKTKMII-LNT 637
+ F ++ G I + S D +L + S + K ++ + T
Sbjct: 157 KYSFPSALQSMRPLG--LSCIPIDMDQFGFLPESMDDILTNWDATSYGSPKPHVLYTIPT 214
Query: 638 PHNPLGKAFTRQELELIADLCKKHNVLCLSDEVYEWM 748
NP G + + + I L +KH+++ L DE Y ++
Sbjct: 215 GQNPTGSTLSVERRKQIYTLAQKHDIIILEDEPYYYL 251
>SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein
Sec23a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 31.5 bits (68), Expect = 0.33
Identities = 18/62 (29%), Positives = 31/62 (50%), Gaps = 3/62 (4%)
Frame = +2
Query: 365 PFNEILVTSGAYEALFSAILGHVDTGDEVIVIEPFFDCYDFMIKSAGGVPKF---IALKP 535
PF ++ S A + +L H D + +I+I+P + F + GGVP +++KP
Sbjct: 582 PFLQVFNNSPDETAFYRHMLNHEDVNNSLIMIQPTLQSFSF--EHPGGVPVLLDAVSVKP 639
Query: 536 KV 541
V
Sbjct: 640 DV 641
>SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 470
Score = 29.1 bits (62), Expect = 1.7
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = +2
Query: 635 TPHNPLGKAFTRQELELIADLCKKHNVLCLSDEVYEWMVYE 757
T NP G T + + L KK++++ + DE Y ++ E
Sbjct: 210 TGQNPTGSTLTLERRKKFLTLAKKYDIIIVEDEPYYFLQME 250
>SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 558
Score = 28.7 bits (61), Expect = 2.3
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = +2
Query: 581 ESELVSLFTSKTKMIILNTPHNPLGKAFTRQELELIADLCKKHNVLCLSDEVYEWMVYEP 760
E++L SL ++ K+I + ++ G E I DL KK+ + DEV+ +Y P
Sbjct: 296 EAKLASLPLNRPKIIAFESVYSMSGNVAPISE---ICDLAKKYGAITFLDEVHAVGMYGP 352
>SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 765
Score = 27.5 bits (58), Expect = 5.3
Identities = 21/81 (25%), Positives = 33/81 (40%), Gaps = 3/81 (3%)
Frame = +2
Query: 308 RLVQNLSKVYSPLIGREIDPFNEILVTSGAYEALFSAILGHVDTGDEVIVIEPFFDCYDF 487
RL+ N + + PF I S + + +L D D +I+I+P Y F
Sbjct: 568 RLLPNFTLYPQFIFHLRRSPFLHIFNNSPDETSFYRHMLNVADVNDSLIMIQPTLQSYSF 627
Query: 488 MIKSAGGVPKF---IALKPKV 541
GVP +++KP V
Sbjct: 628 --NEPEGVPVLLDSVSIKPDV 646
>SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 438
Score = 27.1 bits (57), Expect = 7.0
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +2
Query: 677 LELIADLCKKHNVLCLSDEV 736
LE LCK HNVL ++DEV
Sbjct: 229 LEEAYKLCKAHNVLFIADEV 248
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.1 bits (57), Expect = 7.0
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = -3
Query: 975 LVXXAXGTASAPXCSTGKXSXDXPLGGSSSARPARKPNGSSS 850
L + T+S P S+ S L SSS+ A + SSS
Sbjct: 230 LTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSS 271
>SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 390
Score = 27.1 bits (57), Expect = 7.0
Identities = 13/26 (50%), Positives = 18/26 (69%), Gaps = 1/26 (3%)
Frame = +2
Query: 344 LIGREI-DPFNEILVTSGAYEALFSA 418
L+G+ + DPFN L + YE+LFSA
Sbjct: 289 LLGQSLSDPFNHTLQSFHPYESLFSA 314
>SPAC26F1.10c |pyp1||tyrosine phosphatase Pyp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 550
Score = 26.6 bits (56), Expect = 9.3
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +3
Query: 261 VITLCLTNIPEALVIPD*SRIYRKCIHL 344
V T CL ++P+A PD + +Y+K + L
Sbjct: 240 VQTPCLHSVPDAFTNPDVATLYQKFLRL 267
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,972,749
Number of Sequences: 5004
Number of extensions: 97534
Number of successful extensions: 252
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 251
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 876120908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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