Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_D07_e436_07.seq
(1488 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 200 3e-52
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 85 2e-17
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 85 3e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 61 4e-10
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 27 6.6
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 27 8.8
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 27 8.8
SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 27 8.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 200 bits (489), Expect = 3e-52
Identities = 90/126 (71%), Positives = 111/126 (88%), Gaps = 1/126 (0%)
Frame = +1
Query: 79 GRMSMKEVDEQMLSIQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELF 258
G++SMKEVDEQ+ S+Q KNS+YFVEWIP+NV AVC +PPK LKMS+TFIGN+T+IQE+F
Sbjct: 319 GKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIF 378
Query: 259 KRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQYQDA-TAEDDTEFD 435
+R+ +QFSAMFRRKAFLHWYTGEGMDEMEF EAESN+NDLVSEYQQYQ+A E D +++
Sbjct: 379 RRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGIDEGDEDYE 438
Query: 436 QEDMEE 453
E+ +E
Sbjct: 439 IEEEKE 444
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 85.4 bits (202), Expect = 2e-17
Identities = 40/123 (32%), Positives = 69/123 (56%), Gaps = 8/123 (6%)
Frame = +1
Query: 79 GRMSMKEVDEQMLSIQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSST--------FIGN 234
G + ++V + +I+ K + FV+W P K +CD PP+ ++ S + N
Sbjct: 321 GDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSN 380
Query: 235 TTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQYQDATA 414
TT+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++ +
Sbjct: 381 TTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSM 440
Query: 415 EDD 423
E D
Sbjct: 441 EVD 443
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 84.6 bits (200), Expect = 3e-17
Identities = 42/130 (32%), Positives = 73/130 (56%), Gaps = 9/130 (6%)
Frame = +1
Query: 79 GRMSMKEVDEQMLSIQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSST--------FIGN 234
G + ++V + SI+++ + FV+W P K +C PP+ + S + N
Sbjct: 325 GDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSN 384
Query: 235 TTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQY-QDAT 411
TT+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++ QD+
Sbjct: 385 TTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSM 444
Query: 412 AEDDTEFDQE 441
+ E D+E
Sbjct: 445 DNEMYEADEE 454
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 60.9 bits (141), Expect = 4e-10
Identities = 34/115 (29%), Positives = 61/115 (53%), Gaps = 6/115 (5%)
Frame = +1
Query: 79 GRMSMKEVDEQMLSIQNKNSSYFVEWIPNNVKTAVCDIPP---KGLKMSSTFIGNTTAIQ 249
G +V + +L I+ + + F+ W P +++ A+ P ++S + N T+I
Sbjct: 326 GEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIA 385
Query: 250 ELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESN---VNDLVSEYQQYQD 405
LFKR +Q+ + +R AFL Y E + E + NE +S+ V DL++EY+ +D
Sbjct: 386 SLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYEACED 440
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 27.1 bits (57), Expect = 6.6
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +1
Query: 475 D*IGSIPPIYEILLLKNMVNKYFIV 549
D I ++ PI+++L+LKN + K F+V
Sbjct: 337 DSIPNLGPIHDLLVLKNDIEKSFLV 361
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.6 bits (56), Expect = 8.8
Identities = 19/59 (32%), Positives = 29/59 (49%)
Frame = +1
Query: 226 IGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQYQ 402
+ N + L+K + E+FS +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 26.6 bits (56), Expect = 8.8
Identities = 15/35 (42%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
Frame = +1
Query: 370 NDLVSEYQQ--YQDATAEDDTEFDQEDMEELAQDE 468
NDL E QQ A ++D E D+E E L++DE
Sbjct: 445 NDLADETQQTLLTSALDDEDEEGDEEMEEALSEDE 479
>SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 592
Score = 26.6 bits (56), Expect = 8.8
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +2
Query: 323 ARAWTRWNSTRRRAMSMIWFRNTSSTRTRPLKMTRNL 433
A ++ W+S R+RA+ I F N S + +++ +L
Sbjct: 424 ASSFNVWSSVRKRAIETIEFFNPDSYNVKKIRLLNDL 460
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,111,522
Number of Sequences: 5004
Number of extensions: 69897
Number of successful extensions: 235
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 830530906
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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