Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_D06_e428_08.seq
(1518 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 87 8e-18
SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosacch... 71 4e-13
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 63 1e-10
SPAC637.11 |suv3||ATP-dependent RNA helicase Suv3|Schizosaccharo... 27 9.0
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 86.6 bits (205), Expect = 8e-18
Identities = 42/101 (41%), Positives = 62/101 (61%), Gaps = 7/101 (6%)
Frame = +3
Query: 3 IFPAGGDGLHLVVDESGNFKEENFNTAM-AVLSNAGEXXXXXXXXXXXXXXXXNQ----- 164
+FP+G DG+HLVVDE NF+EENF AM A++ G+ +
Sbjct: 379 LFPSGSDGIHLVVDEKSNFREENFQRAMSALMEKQGDDPAAMATKGNAKKGKTGKGGVKG 438
Query: 165 -TNIFNIVKMIMERNFAPVIIFSFSKKDCELYAMQMASIGL 284
++I+ IVKMIM +N+ PVI+FSFSK++CE A+QM+ + +
Sbjct: 439 PSDIYKIVKMIMVKNYNPVIVFSFSKRECEALALQMSKLDM 479
Score = 83.4 bits (197), Expect = 7e-17
Identities = 47/126 (37%), Positives = 69/126 (54%)
Frame = +1
Query: 169 IYSTLSK*LWKEISHPLSYSVLARKIASCMLCRWRVLDFNTIEEKKLVDEVFNNAMDVLS 348
IY + + K + + +S R+ + L + LD N E+ LV +FNNA++ LS
Sbjct: 442 IYKIVKMIMVKNYNPVIVFSFSKRECEALAL-QMSKLDMNDQTERDLVTTIFNNAVNQLS 500
Query: 349 EEDRKLPQVENVIPXXXXXXXXXXXXXXPILKETIEILFGLGLIKALFATETXAMGFEHA 528
E+DR+LPQ+E+++P PILKE IEILF GL+K LFATET ++G
Sbjct: 501 EKDRELPQIEHILPLLRRGIGIHHSGLLPILKEVIEILFQEGLLKVLFATETFSIGLNMP 560
Query: 529 CEDCCF 546
+ F
Sbjct: 561 AKTVVF 566
Score = 48.8 bits (111), Expect = 2e-06
Identities = 40/109 (36%), Positives = 52/109 (47%)
Frame = +2
Query: 518 LNMPARTVVFTNCQKFXRIGFXIYNIXRVXQMSGRAXKTXVLMIXGXXILXIDSKVXPXG 697
LNMPA+TVVFTN +KF F + QMSGRA + L G IL ID K+ P
Sbjct: 557 LNMPAKTVVFTNVRKFDGKTFRWISGGEYIQMSGRAGRRG-LDDRGIVILMIDEKMDP-P 614
Query: 698 RXKAWVXGKADXXXXXXXXXXXXMVXXLXSXSEGTHSLNXLIEXSFFPF 844
K+ + G+AD ++ L EG S ++E FF F
Sbjct: 615 VAKSMLKGEADRLDSAFHLSYNMILNLL--RVEGI-SPEFMLERCFFQF 660
>SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1030
Score = 70.9 bits (166), Expect = 4e-13
Identities = 40/97 (41%), Positives = 52/97 (53%)
Frame = +1
Query: 277 LDFNTIEEKKLVDEVFNNAMDVLSEEDRKLPQVENVIPXXXXXXXXXXXXXXPILKETIE 456
LD N E K+LV E+F++A++ LSEEDR L Q E + PILKE +E
Sbjct: 393 LDLNDTENKELVTEIFDSAINQLSEEDRGLRQFEEMRSLLLRGIGIHHSGLLPILKELVE 452
Query: 457 ILFGLGLIKALFATETXAMGFEHACEDCCFY*LPKIS 567
ILF GL++ LFATET ++G F K S
Sbjct: 453 ILFQEGLVRILFATETFSIGLNMPARTVLFTKAQKFS 489
Score = 65.3 bits (152), Expect = 2e-11
Identities = 29/94 (30%), Positives = 49/94 (52%)
Frame = +3
Query: 3 IFPAGGDGLHLVVDESGNFKEENFNTAMAVLSNAGEXXXXXXXXXXXXXXXXNQTNIFNI 182
I+P G DG++++VDE FK ENF + VL ++ +++ I
Sbjct: 306 IYPQGADGIYMLVDEKNKFKTENFKKVLEVLDHS----TRQENYSKSSKKVKKSSSLERI 361
Query: 183 VKMIMERNFAPVIIFSFSKKDCELYAMQMASIGL 284
+ M++ + P+I+F FSKK+CE+ A Q + L
Sbjct: 362 INMVLSNRYDPIIVFCFSKKECEINAHQFGKLDL 395
Score = 43.2 bits (97), Expect = 1e-04
Identities = 26/71 (36%), Positives = 35/71 (49%)
Frame = +2
Query: 518 LNMPARTVVFTNCQKFXRIGFXIYNIXRVXQMSGRAXKTXVLMIXGXXILXIDSKVXPXG 697
LNMPARTV+FT QKF F QMSGRA + + G I+ +D +
Sbjct: 473 LNMPARTVLFTKAQKFSGNNFRWLTSGEYMQMSGRAGRRGI-DTKGLSIVILDQSIDEQA 531
Query: 698 RXKAWVXGKAD 730
+ + G+AD
Sbjct: 532 -ARCLMNGQAD 541
>SPCC550.03c |||RNA helicase involved in mRNA
catabolism|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1213
Score = 62.9 bits (146), Expect = 1e-10
Identities = 35/89 (39%), Positives = 44/89 (49%)
Frame = +1
Query: 280 DFNTIEEKKLVDEVFNNAMDVLSEEDRKLPQVENVIPXXXXXXXXXXXXXXPILKETIEI 459
D N +EK V V A+ L +EDR LPQ+ + PI+KE +EI
Sbjct: 574 DLNNHQEKSEVHVVIEKAVARLKKEDRLLPQIGRMREMLSRGLAVHHGGLLPIIKEIVEI 633
Query: 460 LFGLGLIKALFATETXAMGFEHACEDCCF 546
LF GL+K LFATET AMG + F
Sbjct: 634 LFQRGLVKVLFATETFAMGVNMPAKSVVF 662
Score = 33.1 bits (72), Expect = 0.10
Identities = 14/40 (35%), Positives = 25/40 (62%)
Frame = +3
Query: 177 NIVKMIMERNFAPVIIFSFSKKDCELYAMQMASIGL*HHR 296
+++ + ++N PVI+F FSKK CE Y + + L +H+
Sbjct: 540 HLIGHLHKQNLLPVIVFVFSKKRCEEYVDTLTNRDLNNHQ 579
Score = 30.3 bits (65), Expect = 0.73
Identities = 20/68 (29%), Positives = 31/68 (45%)
Frame = +2
Query: 518 LNMPARTVVFTNCQKFXRIGFXIYNIXRVXQMSGRAXKTXVLMIXGXXILXIDSKVXPXG 697
+NMPA++VVF+ QK F Q SGRA + L + G I+ S++
Sbjct: 653 VNMPAKSVVFSGTQKHDGRNFRDLLPGEYTQCSGRAGRRG-LDVTGTVIILSRSELPDTA 711
Query: 698 RXKAWVXG 721
+ + G
Sbjct: 712 SLRHMIMG 719
>SPAC637.11 |suv3||ATP-dependent RNA helicase
Suv3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 26.6 bits (56), Expect = 9.0
Identities = 10/38 (26%), Positives = 23/38 (60%)
Frame = +2
Query: 518 LNMPARTVVFTNCQKFXRIGFXIYNIXRVXQMSGRAXK 631
LN+ + +VF++ +KF + + ++ Q++GRA +
Sbjct: 402 LNLGVKRIVFSDLKKFSGVSTIDIPVPQIKQIAGRAGR 439
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,432,372
Number of Sequences: 5004
Number of extensions: 51137
Number of successful extensions: 117
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 850352646
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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