Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030905E5_D06_e428_08.seq
         (1518 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA he...    93   6e-19
At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative si...    82   8e-16
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila...    51   2e-06
At1g70070.1 68414.m08062 DEAD/DEAH box helicase, putative simila...    34   0.28 
At2g23710.1 68415.m02831 hypothetical protein contains Pfam prof...    32   0.86 
At2g27170.1 68415.m06029 structural maintenance of chromosomes (...    31   1.5  
At1g12280.1 68414.m01420 disease resistance protein (CC-NBS-LRR ...    30   4.6  
At5g62600.1 68418.m07856 transportin-SR-related contains weak si...    29   6.1  

>At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA
           helicase, putative nearly identical to HUA enhancer 2
           [Arabidopsis thaliana] GI:16024936
          Length = 995

 Score = 92.7 bits (220), Expect = 6e-19
 Identities = 44/90 (48%), Positives = 58/90 (64%)
 Frame = +1

Query: 277 LDFNTIEEKKLVDEVFNNAMDVLSEEDRKLPQVENVIPXXXXXXXXXXXXXXPILKETIE 456
           LDFNT EEK++V++VFNNAM  L+EEDR LP +E ++P              P++KE +E
Sbjct: 359 LDFNTDEEKEVVEQVFNNAMQCLNEEDRSLPAIELMLPLLQRGIAVHHSGLLPVIKELVE 418

Query: 457 ILFGLGLIKALFATETXAMGFEHACEDCCF 546
           +LF  GL+KALFATET AMG     +   F
Sbjct: 419 LLFQEGLVKALFATETFAMGLNMPAKTVVF 448



 Score = 72.1 bits (169), Expect = 9e-13
 Identities = 38/97 (39%), Positives = 57/97 (58%), Gaps = 6/97 (6%)
 Frame = +3

Query: 6   FPAGGDGLHLVVDESGNFKEENF----NTAMAVLSNAGEXXXXXXXXXXXXXXXXN--QT 167
           FP GG GL+LVVD++  F+E++F    +T     SN G+                    +
Sbjct: 263 FPMGGGGLYLVVDDNEQFREDSFVKMQDTFPKPKSNDGKKSANGKSGGRGAKGGGGPGDS 322

Query: 168 NIFNIVKMIMERNFAPVIIFSFSKKDCELYAMQMASI 278
           +++ IVKMIMER F PVIIFSFS+++CE +A+ M+ +
Sbjct: 323 DVYKIVKMIMERKFEPVIIFSFSRRECEQHALSMSKL 359



 Score = 34.7 bits (76), Expect = 0.16
 Identities = 31/109 (28%), Positives = 44/109 (40%)
 Frame = +2

Query: 518 LNMPARTVVFTNCQKFXRIGFXIYNIXRVXQMSGRAXKTXVLMIXGXXILXIDSKVXPXG 697
           LNMPA+TVVFT  +K+              QMSGRA +       G  I+ ID ++    
Sbjct: 439 LNMPAKTVVFTAVKKWDGDSHRYIGSGEYIQMSGRAGRRG-KDERGICIIMIDEQM-EMN 496

Query: 698 RXKAWVXGKADXXXXXXXXXXXXMVXXLXSXSEGTHSLNXLIEXSFFPF 844
             +  + GK               +  L S +EG  +   +I  SF  F
Sbjct: 497 TLRDMMLGK-PAPLLSTFRLSYYTILNLLSRAEGQFTAEHVIRHSFHQF 544


>At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative
           similar to SP|P47047 ATP-dependent RNA helicase DOB1
           {Saccharomyces cerevisiae}, HUA enhancer 2 [Arabidopsis
           thaliana] GI:16024936; contains Pfam profiles PF00270:
           DEAD/DEAH box helicase, PF00271: Helicase conserved
           C-terminal domain
          Length = 988

 Score = 82.2 bits (194), Expect = 8e-16
 Identities = 43/121 (35%), Positives = 66/121 (54%)
 Frame = +1

Query: 214 PLSYSVLARKIASCMLCRWRVLDFNTIEEKKLVDEVFNNAMDVLSEEDRKLPQVENVIPX 393
           P+     ++K    +  +   +  N+ +EK  V+ +F +A+D+LS++D+KLPQV N++P 
Sbjct: 322 PVILFSFSKKECEALAMQMSKMVLNSDDEKDAVETIFASAIDMLSDDDKKLPQVSNILPI 381

Query: 394 XXXXXXXXXXXXXPILKETIEILFGLGLIKALFATETXAMGFEHACEDCCFY*LPKISTD 573
                        PILKE IEILF  GLIK LFATET ++G     +   F  + K   D
Sbjct: 382 LKRGIGVHHSGLLPILKEVIEILFQEGLIKCLFATETFSIGLNMPAKTVVFTNVRKFDGD 441

Query: 574 R 576
           +
Sbjct: 442 K 442



 Score = 79.0 bits (186), Expect = 8e-15
 Identities = 40/98 (40%), Positives = 58/98 (59%), Gaps = 4/98 (4%)
 Frame = +3

Query: 3   IFPAGGDGLHLVVDESGNFKEENFNTAMAVLSNAGEXXXXXXXXXXXXXXXXN----QTN 170
           +FPAGG+GL+LVVDE   F E++F  ++  L    E                     +++
Sbjct: 248 VFPAGGNGLYLVVDEKSKFHEDSFQKSLNALVPTNESDKKRDNGKFQKGLVIGKLGEESD 307

Query: 171 IFNIVKMIMERNFAPVIIFSFSKKDCELYAMQMASIGL 284
           IF +VKMI++R + PVI+FSFSKK+CE  AMQM+ + L
Sbjct: 308 IFKLVKMIIQRQYDPVILFSFSKKECEALAMQMSKMVL 345



 Score = 50.8 bits (116), Expect = 2e-06
 Identities = 40/123 (32%), Positives = 53/123 (43%)
 Frame = +2

Query: 518 LNMPARTVVFTNCQKFXRIGFXIYNIXRVXQMSGRAXKTXVLMIXGXXILXIDSKVXPXG 697
           LNMPA+TVVFTN +KF    F   +     QMSGRA +  +    G  IL +D K+ P  
Sbjct: 423 LNMPAKTVVFTNVRKFDGDKFRWLSSGEYIQMSGRAGRRGIDK-RGICILMVDEKMEP-A 480

Query: 698 RXKAWVXGKADXXXXXXXXXXXXMVXXLXSXSEGTHSLNXLIEXSFFPFSXXAXFXXXXK 877
             K+ + G AD            ++  L    EG      L+  SFF F          K
Sbjct: 481 VAKSMLKGSADSLNSAFHLSYNMLLNQLRC-EEG--DPENLLRNSFFQFQADRAIPDLEK 537

Query: 878 KSK 886
           + K
Sbjct: 538 QIK 540


>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar
           to SP|P35207 Antiviral protein SKI2 {Saccharomyces
           cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
           box helicase, PF00271: Helicase conserved C-terminal
           domain
          Length = 1347

 Score = 50.8 bits (116), Expect = 2e-06
 Identities = 29/79 (36%), Positives = 38/79 (48%)
 Frame = +1

Query: 280 DFNTIEEKKLVDEVFNNAMDVLSEEDRKLPQVENVIPXXXXXXXXXXXXXXPILKETIEI 459
           D  +  EK  +    + A   L   DR LPQV  +                PI+KE +E+
Sbjct: 664 DLTSSSEKSEIRVFCDKAFSRLKGSDRNLPQVLRLQSLLHRGIGVHHAGLLPIVKEVVEM 723

Query: 460 LFGLGLIKALFATETXAMG 516
           LF  G+IK LF+TET AMG
Sbjct: 724 LFCRGVIKVLFSTETFAMG 742


>At1g70070.1 68414.m08062 DEAD/DEAH box helicase, putative similar
           to SP|P35207 Antiviral protein SKI2 {Saccharomyces
           cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
           box helicase, PF00271: Helicase conserved C-terminal
           domain
          Length = 1171

 Score = 33.9 bits (74), Expect = 0.28
 Identities = 16/28 (57%), Positives = 19/28 (67%)
 Frame = +1

Query: 433 PILKETIEILFGLGLIKALFATETXAMG 516
           P+ K  IE LF  GL+K +FATET A G
Sbjct: 507 PLWKSFIEELFQRGLVKVVFATETLAAG 534


>At2g23710.1 68415.m02831 hypothetical protein contains Pfam profile
           PF03384: Drosophila protein of unknown function, DUF287;
            this may be a pseudogene;  the protein appears slightly
           truncated on the 3' end due to intervening stop codon.
          Length = 592

 Score = 32.3 bits (70), Expect = 0.86
 Identities = 13/57 (22%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
 Frame = +1

Query: 214 PLSYSVLARKIASCMLCRWRVLDFNTIEEKKLVDEVFNNAMDV-LSEEDRKLPQVEN 381
           P+ YS+    + +C+ CR    ++ T+   K V+ +F    D+ + + + KL + ++
Sbjct: 231 PIGYSIREHALLTCLNCREYPKNYKTLGSLKFVERLFKRTEDIKIKDVEEKLEEFKS 287


>At2g27170.1 68415.m06029 structural maintenance of chromosomes
           (SMC) family protein similar to basement
           membrane-associated chondroitin proteoglycan Bamacan
           [Rattus norvegicus] GI:1785540; contains Pfam profile
           PF02463: RecF/RecN/SMC N terminal domain. No suitalble
           start codon was identified.
          Length = 1207

 Score = 31.5 bits (68), Expect = 1.5
 Identities = 16/36 (44%), Positives = 23/36 (63%)
 Frame = +1

Query: 271 RVLDFNTIEEKKLVDEVFNNAMDVLSEEDRKLPQVE 378
           RVLD NT++E+KL DE+     D L+E D  + + E
Sbjct: 404 RVLDSNTVQEQKLQDEILRLNTD-LTERDEHIKKHE 438


>At1g12280.1 68414.m01420 disease resistance protein (CC-NBS-LRR
           class), putative domain signature CC-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 894

 Score = 29.9 bits (64), Expect = 4.6
 Identities = 22/57 (38%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
 Frame = +1

Query: 232 LARKIASCMLCRWRVLDFNTIEE----KKLVDEVFNNAMDVLSEEDRKLPQVENVIP 390
           LARK+A    C    L  N I E    K++V E + NA+DVLS    + P +E ++P
Sbjct: 340 LARKVAG--KCCGLPLALNVIGETMACKRMVQE-WRNAIDVLSSYAAEFPGMEQILP 393


>At5g62600.1 68418.m07856 transportin-SR-related contains weak
           similarity to transportin-SR (GI:5052414) [Homo sapiens]
          Length = 958

 Score = 29.5 bits (63), Expect = 6.1
 Identities = 21/102 (20%), Positives = 42/102 (41%), Gaps = 1/102 (0%)
 Frame = +1

Query: 238 RKIASCMLCRWRVLDFNTIEEKKLVDEVFNNAMDVLSEEDRKLPQVENVIPXXXXXXXXX 417
           R   + + C W + ++ ++ E +++ +V     ++   +  +L Q   ++          
Sbjct: 451 RPAEAILFCIWAISNYVSVVEAEVMPQVMALLQNL--PQQAQLLQTACLLVGAYSKWLNA 508

Query: 418 XXXXXPILKETIEILF-GLGLIKALFATETXAMGFEHACEDC 540
                 IL   I IL  G+G  +   A    A+ F H C+DC
Sbjct: 509 APASVSILPSIIRILMSGMGTSEDCAAA--AALAFRHTCDDC 548


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,669,945
Number of Sequences: 28952
Number of extensions: 299330
Number of successful extensions: 575
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 522
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 573
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4057847232
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)