BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030905E5_D04_e412_08.seq
(1545 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 31 0.56
SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosacc... 29 1.3
SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces... 29 1.7
SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein L15b|Schizos... 29 1.7
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 27 5.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 9.2
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 9.2
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 30.7 bits (66), Expect = 0.56
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = -3
Query: 325 RPIYRELAQVLQQNNSNRCCRLHRSQCPSRRGMKSGIEYHQLWVVQSPPA 176
+P+ R+ + QN S L+ C + +K GIE W+ QSP A
Sbjct: 455 QPVTRDFL-LAAQNASGIYLNLNEQYCLVMKDLKDGIEMKPKWLTQSPAA 503
>SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase
Ubp3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 512
Score = 29.5 bits (63), Expect = 1.3
Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = +1
Query: 772 HFLTNRPKSAKSLIXQKNRPEIGLSGCSSLEQESTIKRT-WTXTSRGEKPSSGRWPLREP 948
+ L N ++ KS I N + SG S + +S+++ + WT + +KP R E
Sbjct: 251 YLLKNDERNPKSDIKISNGIK---SGLDSFDDQSSVEASGWTEVGKNKKPVIARSATVER 307
Query: 949 SP*IKFLGXRCRKXL 993
SP + G + R L
Sbjct: 308 SPISQIFGGQLRSTL 322
>SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 201
Score = 29.1 bits (62), Expect = 1.7
Identities = 19/52 (36%), Positives = 25/52 (48%)
Frame = +2
Query: 821 RIDPR*G*VVVPVWNKSPLLKERGLQRQGAKNRLQGDGXYVNHHPKSSFWXR 976
R DPR +V PV + RGL G K+R G G N+ P+ + W R
Sbjct: 143 RRDPRINWIVNPVHKHR---ESRGLTSIGKKSRGIGKGHRFNNSPQHATWLR 191
>SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein
L15b|Schizosaccharomyces pombe|chr 1|||Manual
Length = 201
Score = 29.1 bits (62), Expect = 1.7
Identities = 19/52 (36%), Positives = 25/52 (48%)
Frame = +2
Query: 821 RIDPR*G*VVVPVWNKSPLLKERGLQRQGAKNRLQGDGXYVNHHPKSSFWXR 976
R DPR +V PV + RGL G K+R G G N+ P+ + W R
Sbjct: 143 RRDPRINWIVNPVHKHR---ESRGLTSIGKKSRGIGKGHRYNNSPQHATWLR 191
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 27.5 bits (58), Expect = 5.2
Identities = 17/41 (41%), Positives = 21/41 (51%)
Frame = +1
Query: 760 VKSAHFLTNRPKSAKSLIXQKNRPEIGLSGCSSLEQESTIK 882
VK FLTN + SL+ Q NRP S S +E TI+
Sbjct: 675 VKDYDFLTNLNATTLSLLTQSNRPSTLFS--SDIEYTPTIQ 713
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 26.6 bits (56), Expect = 9.2
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
Frame = +3
Query: 522 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 686
P P+ S ++ H + + +L N I+L ++PLSP A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231
Query: 687 SCA 695
S A
Sbjct: 232 SDA 234
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 26.6 bits (56), Expect = 9.2
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 629 PPFASWRNSEEARTDRPSQQLRSLN 703
PP AS RN E + PS+Q S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,236,366
Number of Sequences: 5004
Number of extensions: 76294
Number of successful extensions: 150
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 868192212
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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