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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030905E5_C09_e451_05.seq
         (1577 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str...   205   5e-53
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S...   204   9e-53
At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar...    31   1.6  
At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family...    30   4.8  
At2g18470.1 68415.m02151 protein kinase family protein contains ...    29   6.4  
At4g08380.1 68417.m01384 proline-rich extensin-like family prote...    29   8.4  

>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
           similarity to 60S ribosomal protein L1 GB:P49691
          Length = 406

 Score =  205 bits (501), Expect = 5e-53
 Identities = 113/278 (40%), Positives = 154/278 (55%), Gaps = 3/278 (1%)
 Frame = +3

Query: 84  ARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVSKEAGH 257
           ARPLV++ +   ++    S  + LP V  AP+RPD+VN VH  +S N+RQPY VSK+AGH
Sbjct: 6   ARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGH 65

Query: 258 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 437
           QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH        
Sbjct: 66  QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 125

Query: 438 XXXXXXXXXXXXXXXXXQARGHIVEKIPELPLVVSDKVQEINKTKQAVIFLRXIKAWF*X 617
                             ARGH +E +PE+PLVVSD  + + KT  A+  L+ I A+   
Sbjct: 126 RHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDA 185

Query: 618 P*SVQITAPACR*REKCXXVVVXNAKDPLIIX**GQGS*LVH-FXNIPGIQMLNXNKXKX 794
               + +      + K       + K PL++    +GS +V  F N+PG+++ +  +   
Sbjct: 186 E-KAKNSIGIRPGKGKMRNRRYISRKGPLVVY-GTEGSKIVKAFRNLPGVELCHVERLNL 243

Query: 795 PEXGTRXXILXXLLFGTXPFSXA*PVVXLLEXXSXKKK 908
            +      +   +++    F     +    E  S KKK
Sbjct: 244 LKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKK 281



 Score = 32.7 bits (71), Expect = 0.68
 Identities = 27/95 (28%), Positives = 33/95 (34%)
 Frame = +2

Query: 623 KCTNHSACVPVKGKMXXRXRVQRKGPPYHXIIRTRVLTRAFXKHSWHPDAXREQXETS*X 802
           K  N     P KGKM  R  + RKGP          + +AF           E+      
Sbjct: 187 KAKNSIGIRPGKGKMRNRRYISRKGPLVVYGTEGSKIVKAFRNLPGVELCHVERLNLL-K 245

Query: 803 WXPXXHLXGFIIWXXSLFXGLTRCXAPGKXXXEKK 907
             P  HL  F+IW  S F  L       +   EKK
Sbjct: 246 LAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKK 280


>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
           roibosomal protein L4, Arabidopsis thaliana,
           EMBL:CAA79104
          Length = 407

 Score =  204 bits (499), Expect = 9e-53
 Identities = 113/278 (40%), Positives = 153/278 (55%), Gaps = 3/278 (1%)
 Frame = +3

Query: 84  ARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVSKEAGH 257
           ARPLV+V     ++    S  + LP V  AP+RPD+VN VH  +S N+RQPY VSK+AGH
Sbjct: 7   ARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGH 66

Query: 258 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 437
           QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH        
Sbjct: 67  QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 126

Query: 438 XXXXXXXXXXXXXXXXXQARGHIVEKIPELPLVVSDKVQEINKTKQAVIFLRXIKAWF*X 617
                             ARGH +E +PE+PLVVSD  + + KT  A+  L+ I A+   
Sbjct: 127 RHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDA 186

Query: 618 P*SVQITAPACR*REKCXXVVVXNAKDPLIIX**GQGS*LVH-FXNIPGIQMLNXNKXKX 794
               + +      + K       + K PL++    +G+ +V  F N+PG+++ +  +   
Sbjct: 187 E-KAKNSIGIRPGKGKMRNRRYISRKGPLVVF-GTEGAKIVKAFRNLPGVELCHVERLNL 244

Query: 795 PEXGTRXXILXXLLFGTXPFSXA*PVVXLLEXXSXKKK 908
            +      +   +++    F     +    E  S KKK
Sbjct: 245 LKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKK 282



 Score = 32.7 bits (71), Expect = 0.68
 Identities = 27/95 (28%), Positives = 33/95 (34%)
 Frame = +2

Query: 623 KCTNHSACVPVKGKMXXRXRVQRKGPPYHXIIRTRVLTRAFXKHSWHPDAXREQXETS*X 802
           K  N     P KGKM  R  + RKGP          + +AF           E+      
Sbjct: 188 KAKNSIGIRPGKGKMRNRRYISRKGPLVVFGTEGAKIVKAFRNLPGVELCHVERLNLL-K 246

Query: 803 WXPXXHLXGFIIWXXSLFXGLTRCXAPGKXXXEKK 907
             P  HL  F+IW  S F  L       +   EKK
Sbjct: 247 LAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKK 281


>At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar to
           gb|U06698 neuronal kinesin heavy chain from Homo sapiens
           and contains a PF|00225 Kinesin motor domain. EST
           gb|AA042507 comes from this gene; identical to cDNA
           MKRP1 mRNA for kinesin-related protein,  GI:16902291,
           kinesin-related protein [Arabidopsis thaliana]
           GI:16902292
          Length = 890

 Score = 31.5 bits (68), Expect = 1.6
 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
 Frame = -2

Query: 199 WTSLTRS-GLMGALNTNGRGSEAPATTSLFSEYTDTK 92
           W  L +S G+ G L T+G  S+A  + S  S+YT TK
Sbjct: 549 WLKLKKSDGVAGTLPTDGNQSQASGSPSSSSKYTQTK 585


>At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family
           protein contains proline-rich extensin domains,
           INTERPRO:IPR002965
          Length = 340

 Score = 29.9 bits (64), Expect = 4.8
 Identities = 14/49 (28%), Positives = 22/49 (44%)
 Frame = -2

Query: 412 CQRLHGLVGANIRPPRHMLPNAPCPDLWVPPPRTRGIRATARPVPQDSA 266
           CQ+  G V   +  P H +P      L  PPP++  +   + P P  S+
Sbjct: 169 CQQSLGFVAPMMAQPMHQIPTTIVLSLPPPPPQSLSLSLPSPPQPPPSS 217


>At2g18470.1 68415.m02151 protein kinase family protein contains
           Pfam PF00069: Protein kinase domain
          Length = 633

 Score = 29.5 bits (63), Expect = 6.4
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = -1

Query: 518 NLLNNMPSSLNEGRYPGRCHGGGHGSTSLP*VHATVPAPP 399
           N  NN PS  ++G    R +GG +G    P    + P+PP
Sbjct: 66  NSSNNSPSPPSQGGGGERGNGGNNGGNDTPPSRGSPPSPP 105


>At4g08380.1 68417.m01384 proline-rich extensin-like family protein
           contains proline-rich extensin domains,
           INTERPRO:IPR002965
          Length = 437

 Score = 29.1 bits (62), Expect = 8.4
 Identities = 10/19 (52%), Positives = 12/19 (63%)
 Frame = -2

Query: 373 PPRHMLPNAPCPDLWVPPP 317
           PP    P  PCPD++ PPP
Sbjct: 385 PPYAYSPPPPCPDVYKPPP 403


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,317,287
Number of Sequences: 28952
Number of extensions: 317860
Number of successful extensions: 889
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 827
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 884
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4250619072
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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