BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030905E5_C03_e403_05.seq (1654 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 40 8e-04 SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase Cka1|Sch... 31 0.46 SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 31 0.61 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 28 3.3 SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 27 5.7 SPAC1D4.01 ||SPAC1F3.11|sequence orphan|Schizosaccharomyces pomb... 27 7.5 >SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 604 Score = 40.3 bits (90), Expect = 8e-04 Identities = 25/88 (28%), Positives = 42/88 (47%), Gaps = 1/88 (1%) Frame = +2 Query: 461 RSRDRHSHK-KDKSLEKEAHKDYRSKSRGXXXXXXXXXXXXXXXXTVFCMQLSQRIRAKD 637 RS H + +D + +H+ RS+SR TVF QL+ R+ +++ Sbjct: 197 RSSRAHDYDYEDLRDDDRSHERKRSRSRPRERSSKLSEEERDRR-TVFVSQLANRLTSRE 255 Query: 638 LEEFFSSVGKVRDVRMITCXXTRRFKGI 721 L +FF G VRD +++ + R KG+ Sbjct: 256 LYDFFEQAGPVRDAQIVRDKISGRSKGV 283 >SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase Cka1|Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 31.1 bits (67), Expect = 0.46 Identities = 32/127 (25%), Positives = 58/127 (45%), Gaps = 5/127 (3%) Frame = +2 Query: 167 NERTSTPISTKKRIKKAVEDAAGQEIEEDQEIK-ILNVQET-EMIRKIKNVKRKGT---V 331 N+ + P+ + R+ V + +E + + ++ + Q+ E+IRK+ K + Sbjct: 2 NQTEAAPVVSVSRVYAHVNEEMPREYWDYENMQEVFGYQDNYEIIRKVGRGKYSEVFEGL 61 Query: 332 NVVIGIKIADTEIKNVKRKKIVNEIKTKXKDGARIKNVVLIRDRSRDRHSHKKDKSLEKE 511 NV+ K +K VK KKI EIK ++ A N++ + D RD S E Sbjct: 62 NVLNNSKCIIKVLKPVKYKKIKREIKI-LQNLAGGPNIISLLDIVRDPESKTPSLIFEFV 120 Query: 512 AHKDYRS 532 + D+R+ Sbjct: 121 DNIDFRT 127 >SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosaccharomyces pombe|chr 3|||Manual Length = 822 Score = 30.7 bits (66), Expect = 0.61 Identities = 31/122 (25%), Positives = 55/122 (45%), Gaps = 1/122 (0%) Frame = +2 Query: 161 QSNERTSTPI-STKKRIKKAVEDAAGQEIEEDQEIKILNVQETEMIRKIKNVKRKGTVNV 337 + E + P+ KK KKA A + +D + L+ +E+E K K K+K Sbjct: 101 EEEEDSEKPVRKNKKSSKKASPKNAFDALADDMDDLSLDEEESESSEKSKKKKKKSKSKD 160 Query: 338 VIGIKIADTEIKNVKRKKIVNEIKTKXKDGARIKNVVLIRDRSRDRHSHKKDKSLEKEAH 517 + D +I++ ++ K + K+K D A K+ + R ++ + K LE E + Sbjct: 161 DGSEALDDGDIESSEKDK-KKKKKSKENDDAPKKD---RKTRKKEEKARKLASMLESE-N 215 Query: 518 KD 523 KD Sbjct: 216 KD 217 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 28.3 bits (60), Expect = 3.3 Identities = 12/39 (30%), Positives = 19/39 (48%) Frame = -1 Query: 319 PFHVLYFSYHLCLLDVENLYLLIFFYLLTGCVFYCFLYP 203 PFH+++ Y LC+ + L + LL C + YP Sbjct: 556 PFHMVFECYKLCINEFTPFDLEVLALLLESCGRFLLRYP 594 >SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 674 Score = 27.5 bits (58), Expect = 5.7 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +2 Query: 593 TVFCMQLSQRIRAKDLEEFFSSVGKVRDVRMITCXXTRRFKG 718 T+F L+ + + DL FFS VG ++ ++T T +G Sbjct: 6 TLFVRNLAFQTKQDDLTNFFSDVGPIKHAVVVTNPETGENRG 47 >SPAC1D4.01 ||SPAC1F3.11|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 27.1 bits (57), Expect = 7.5 Identities = 25/120 (20%), Positives = 50/120 (41%), Gaps = 13/120 (10%) Frame = +2 Query: 209 KKAVEDAAGQEIEEDQEIKILNVQETEMIRKIKN---------VKRKGTVNVV--IGIKI 355 KK ++ Q E+ E LN + ++ IKN ++ + + + + I Sbjct: 155 KKLKQERLAQNYSENGETNALNTKNESTVQNIKNSLHPNEHSFIRDAAALGAIREVDLGI 214 Query: 356 ADTEIKNVKRKKIVNEIKTKXKDGARIKNVVLIRDRSRDRHSHK--KDKSLEKEAHKDYR 529 T++ N+K + + + + K+ K + D +RD K K S ++E+ YR Sbjct: 215 ISTDVDNLKNGRKRQKKRARMKEKLDSKALRTSEDAARDEFIEKMLKPISQDEESKGIYR 274 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,738,262 Number of Sequences: 5004 Number of extensions: 55318 Number of successful extensions: 193 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 188 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 193 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 939550476 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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