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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030905E5_B06_e426_04.seq
         (1530 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g73230.1 68414.m08475 nascent polypeptide-associated complex ...   134   2e-31
At1g17880.1 68414.m02212 nascent polypeptide-associated complex ...   133   3e-31
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r...    30   4.6  

>At1g73230.1 68414.m08475 nascent polypeptide-associated complex
           (NAC) domain-containing protein similar to SP|P20290
           Transcription factor BTF3 (RNA polymerase B
           transcription factor 3) {Homo sapiens}; contains Pfam
           profile PF01849: NAC domain
          Length = 165

 Score =  134 bits (323), Expect = 2e-31
 Identities = 72/147 (48%), Positives = 92/147 (62%), Gaps = 11/147 (7%)
 Frame = +3

Query: 231 MNTEKLKKLQSQVRIGGKGTPRRKKKVVHVTAATDDXXXXXXXXXXXVNTIPGIEEVNMI 410
           MN EKL K+ + VR GGKGT RRKKK VH T  TDD           VN+IP IEEVN+ 
Sbjct: 1   MNREKLMKMANTVRTGGKGTVRRKKKAVHKTTTTDDKRLQSTLKRVGVNSIPAIEEVNIF 60

Query: 411 KDDGTVIHFNNPKAQASLAANTFAITGHGENKQIAEMLPGILSQLGPEGLNQLKRLASSV 590
           KDD  VI F NPK QAS+AANT+ ++G  + K++ ++LP I+SQLGP+ L+ LK+LA   
Sbjct: 61  KDD-VVIQFINPKVQASIAANTWVVSGTPQTKKLQDILPQIISQLGPDNLDNLKKLAEQF 119

Query: 591 AAPKP-----------LDEDDEVPNLV 638
               P            D+DD+VP+LV
Sbjct: 120 QKQAPGAGDVPATIQEEDDDDDVPDLV 146


>At1g17880.1 68414.m02212 nascent polypeptide-associated complex
           (NAC) domain-containing protein / BTF3b-like
           transcription factor, putative similar to SP|P20290
           Transcription factor BTF3 (RNA polymerase B
           transcription factor 3) {Homo sapiens}; contains Pfam
           profile PF01849: NAC domain; identical to cDNA
           BTF3b-like factor GI:5912423
          Length = 165

 Score =  133 bits (322), Expect = 3e-31
 Identities = 72/154 (46%), Positives = 97/154 (62%), Gaps = 11/154 (7%)
 Frame = +3

Query: 231 MNTEKLKKLQSQVRIGGKGTPRRKKKVVHVTAATDDXXXXXXXXXXXVNTIPGIEEVNMI 410
           MN EKL K+ + VR GGKGT RRKKK VH T  TDD           VN+IP IEEVN+ 
Sbjct: 1   MNREKLMKMANTVRTGGKGTVRRKKKAVHKTNTTDDKRLQSTLKRIGVNSIPAIEEVNIF 60

Query: 411 KDDGTVIHFNNPKAQASLAANTFAITGHGENKQIAEMLPGILSQLGPEGLNQLKRLA--- 581
           KDD  VI F NPK QAS+AANT+ ++G  + K++ ++LP I+SQLGP+ ++ LK+LA   
Sbjct: 61  KDD-VVIQFINPKVQASIAANTWVVSGSPQTKKLQDILPQIISQLGPDNMDNLKKLAEQF 119

Query: 582 --------SSVAAPKPLDEDDEVPNLVGNFDEAS 659
                   ++ +A    ++DD+VP LVG   E +
Sbjct: 120 QKQASGEGNAASATIQEEDDDDVPELVGETFETA 153


>At3g59820.1 68416.m06675 calcium-binding mitochondrial
           protein-related contains weak similarity to
           Calcium-binding mitochondrial protein Anon-60Da
           (Swiss-Prot:P91927) [Drosophila melanogaster]
          Length = 755

 Score = 29.9 bits (64), Expect = 4.6
 Identities = 19/45 (42%), Positives = 27/45 (60%), Gaps = 4/45 (8%)
 Frame = +3

Query: 537 SQLGPE-GLNQLK---RLASSVAAPKPLDEDDEVPNLVGNFDEAS 659
           S++GP  G+  +    R A++VAA KP +ED +V  L  N  EAS
Sbjct: 94  SEVGPSLGMRYMSLSIRNATTVAAKKPEEEDKKVDELAKNRKEAS 138


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,402,829
Number of Sequences: 28952
Number of extensions: 400695
Number of successful extensions: 769
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 765
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4096401600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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