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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030905E5_A10_e457_02.seq
         (1515 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.24c |atg8||autophagy associated protein Atg8 |Schizosacc...   159   7e-40
SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c...    29   1.3  
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    27   5.1  

>SPBP8B7.24c |atg8||autophagy associated protein Atg8
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score =  159 bits (387), Expect = 7e-40
 Identities = 68/113 (60%), Positives = 90/113 (79%)
 Frame = +2

Query: 173 QYKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFL 352
           Q+K++ SFEKRKTE ++IR KYPDR+PVI EK  K+ +  +DKKKYLVPSDLTVGQF ++
Sbjct: 4   QFKDDFSFEKRKTESQRIREKYPDRIPVICEKVDKSDIAAIDKKKYLVPSDLTVGQFVYV 63

Query: 353 IRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 511
           IRKRI L PE A+F F++ ++PPT+A M ++Y+EH  ED FLYI +S EN +G
Sbjct: 64  IRKRIKLSPEKAIFIFIDEILPPTAALMSTIYEEHKSEDGFLYITYSGENTFG 116


>SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 598

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 13/33 (39%), Positives = 15/33 (45%)
 Frame = +1

Query: 1204 SQSTXGPXNLREPXPXKXKPGXLAXSXPFXPPP 1302
            S S  GP    +P     KPG  A + P  PPP
Sbjct: 384  STSKTGPLFGNKPADPSAKPGATASTTPSEPPP 416


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 27.5 bits (58), Expect = 5.1
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +1

Query: 886 PPFASWRNSEEARTDRPSQQXRSLN 960
           PP AS RN  E  +  PS+Q  S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,786,761
Number of Sequences: 5004
Number of extensions: 89450
Number of successful extensions: 183
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 848370472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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