Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_H12_e672_16.seq
(1466 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 387 e-108
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 220 3e-58
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 219 1e-57
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 203 6e-53
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 29 1.2
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 4.9
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 6.5
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 6.5
SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom... 27 6.5
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 8.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 387 bits (953), Expect = e-108
Identities = 174/253 (68%), Positives = 203/253 (80%)
Frame = +3
Query: 165 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 344
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 345 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 524
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 525 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 704
R+E+E+CD LQGFQ KIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 705 EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLXHLVSLTMSGVTTXL 884
EPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y DL HLVS M+GVTT
Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSF 240
Query: 885 RFPGQLNADLRNL 923
RFPG+LN+DLR L
Sbjct: 241 RFPGELNSDLRKL 253
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 220 bits (538), Expect = 3e-58
Identities = 103/256 (40%), Positives = 151/256 (58%), Gaps = 6/256 (2%)
Frame = +3
Query: 165 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 326
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 327 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 506
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 507 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 686
SVL+ +R+ +++C LQGF ++ EY + +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 687 VSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLXHLVSLTMS 866
VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY +L L++ +S
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVS 240
Query: 867 GVTTXLRFPGQLNADL 914
+T LRF G LN DL
Sbjct: 241 SITASLRFAGSLNVDL 256
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 219 bits (534), Expect = 1e-57
Identities = 103/252 (40%), Positives = 148/252 (58%), Gaps = 2/252 (0%)
Frame = +3
Query: 165 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 338
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 339 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 518
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 519 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 698
+R+ +++C LQGF ++ EY + +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 699 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLXHLVSLTMSGVTT 878
VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y +L L++ +S +T
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 879 XLRFPGQLNADL 914
LRF G LN DL
Sbjct: 241 SLRFEGSLNVDL 252
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 203 bits (495), Expect = 6e-53
Identities = 97/263 (36%), Positives = 153/263 (58%), Gaps = 3/263 (1%)
Frame = +3
Query: 168 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 347
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 348 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 521
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 522 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 698
+ +E++ D L+GF ++ + YP +I+ TYSV P S VSD
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 699 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLXHLVSLTMSGVTT 878
VV+PYN+ L++ +L N D +DN AL I L PT+ LVS MS TT
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTT 241
Query: 879 XLRFPGQLNADLRNLPXTWFXSP 947
LR+PG +N DL ++ + SP
Sbjct: 242 TLRYPGYMNNDLVSIIASLIPSP 264
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 29.5 bits (63), Expect = 1.2
Identities = 29/87 (33%), Positives = 44/87 (50%), Gaps = 7/87 (8%)
Frame = +1
Query: 574 TLSE-AVPAPVWAHYLSQRSVKSTPTES*THTQSSP----RPKYQTPSSNPTMRLSQCTN 738
T+S+ AVP+ +A+ +S RSV S T+S T++SP K S T+ S TN
Sbjct: 643 TVSDTAVPSMSFANNISSRSVISAATDSKPSTRTSPPFVNNTKPIVAKSPVTVTASSETN 702
Query: 739 --S*KTQTKHTVSITRHSTIFAFARSN 813
S K K + ++ + +F RSN
Sbjct: 703 KKSQKINKKASPRVSLWTKLFGKFRSN 729
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 4.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 468 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 557
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
Mei2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 750
Score = 27.1 bits (57), Expect = 6.5
Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
Frame = +1
Query: 406 SDRYSALTTLFSDSPAPVTTGPRDTTQRALS*STQFLT*SEK-NPNHVIVYKDSNSHTLS 582
++ Y +TL P+P+ T+ + S S +LT ++ PN V + N++ S
Sbjct: 45 NEHYPPKSTLLLSGPSPIRNIQLSATKSSESNSIDYLTDTQNIFPNFV---NNENNYQFS 101
Query: 583 EAVPAPVWAHYLSQRSVKST 642
A P+ A + +R V +T
Sbjct: 102 TAPLNPIDACRVGERKVFTT 121
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 6.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +3
Query: 204 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 305
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPCC777.15 |||tRNA dihydrouridine synthase Dus4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 326
Score = 27.1 bits (57), Expect = 6.5
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2
Query: 827 LWRSXSLSLPYHVWRHHL 880
LW S S SL +H++ HHL
Sbjct: 257 LWYSTSYSLNFHLFYHHL 274
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 8.6
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 399 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 500
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,325,361
Number of Sequences: 5004
Number of extensions: 78845
Number of successful extensions: 215
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 816655688
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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