Samia BLAST Result against mosquito

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030731E7_H08_e640_16.seq
         (1524 letters)

Database: mosquito 
           2352 sequences; 563,979 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY027891-1|AAK15783.1|  801|Anopheles gambiae collagen IV alpha ...    29   0.27 
AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ...    29   0.27 

>AY027891-1|AAK15783.1|  801|Anopheles gambiae collagen IV alpha 1
            chain precursor protein.
          Length = 801

 Score = 29.5 bits (63), Expect = 0.27
 Identities = 15/36 (41%), Positives = 16/36 (44%)
 Frame = -2

Query: 1322 PKKXGXPGXPGEXXXRGXPGXPPPGNXPXGXAXPXG 1215
            P + G PG PGE    G  G P P   P G   P G
Sbjct: 609  PGERGYPGMPGEDGTPGLRGEPGPKGEP-GLLGPPG 643



 Score = 27.9 bits (59), Expect = 0.82
 Identities = 13/28 (46%), Positives = 15/28 (53%)
 Frame = -2

Query: 1331 PRXPKKXGXPGXPGEXXXRGXPGXPPPG 1248
            P+ P+  G PG PG    RG PG P  G
Sbjct: 478  PQGPR--GYPGQPGPEGLRGEPGQPGYG 503



 Score = 27.1 bits (57), Expect = 1.4
 Identities = 13/29 (44%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
 Frame = -2

Query: 1331 PRXPKKXGXPGXPGEXXXRGXPG-XPPPG 1248
            P  P + G PG  GE   +G PG   PPG
Sbjct: 615  PGMPGEDGTPGLRGEPGPKGEPGLLGPPG 643



 Score = 25.8 bits (54), Expect = 3.3
 Identities = 9/18 (50%), Positives = 10/18 (55%)
 Frame = -2

Query: 1310 GXPGXPGEXXXRGXPGXP 1257
            G PG PG    +G PG P
Sbjct: 544  GAPGLPGRDGEKGEPGRP 561



 Score = 25.0 bits (52), Expect = 5.8
 Identities = 11/24 (45%), Positives = 11/24 (45%)
 Frame = -2

Query: 1310 GXPGXPGEXXXRGXPGXPPPGNXP 1239
            G PG  GE   RG PG P     P
Sbjct: 238  GLPGPQGEVGPRGFPGRPGEKGVP 261



 Score = 25.0 bits (52), Expect = 5.8
 Identities = 10/22 (45%), Positives = 11/22 (50%)
 Frame = -2

Query: 1316 KXGXPGXPGEXXXRGXPGXPPP 1251
            + G  G PG    RG PG P P
Sbjct: 469  ESGSVGMPGPQGPRGYPGQPGP 490



 Score = 24.6 bits (51), Expect = 7.6
 Identities = 15/38 (39%), Positives = 16/38 (42%), Gaps = 2/38 (5%)
 Frame = -2

Query: 1322 PKKXGXPGXPGEXXXRGXPGXPPPGNXP--XGXAXPXG 1215
            P + G  G PGE   RG PG P     P   G   P G
Sbjct: 600  PGRPGASGVPGE---RGYPGMPGEDGTPGLRGEPGPKG 634



 Score = 24.6 bits (51), Expect = 7.6
 Identities = 10/24 (41%), Positives = 10/24 (41%)
 Frame = -2

Query: 1310 GXPGXPGEXXXRGXPGXPPPGNXP 1239
            G  G PGE    G PG P     P
Sbjct: 700  GDKGRPGEAGIDGAPGAPGKDGLP 723


>AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1
            chain protein.
          Length = 1024

 Score = 29.5 bits (63), Expect = 0.27
 Identities = 15/40 (37%), Positives = 17/40 (42%), Gaps = 1/40 (2%)
 Frame = -2

Query: 1331 PRXPKKXGXPGXPGEXXXRGXPGXPP-PGNXPXGXAXPXG 1215
            P  P K G  G PG    +G PG P      P G + P G
Sbjct: 552  PGRPGKTGRDGPPGLTGEKGEPGLPVWKDRGPSGPSGPLG 591



 Score = 27.5 bits (58), Expect = 1.1
 Identities = 11/30 (36%), Positives = 13/30 (43%)
 Frame = -2

Query: 1310 GXPGXPGEXXXRGXPGXPPPGNXPXGXAXP 1221
            G PG PG+   +G PG   P   P     P
Sbjct: 149  GPPGYPGDVGPKGEPGPKGPAGHPGAPGRP 178



 Score = 26.2 bits (55), Expect = 2.5
 Identities = 14/39 (35%), Positives = 16/39 (41%)
 Frame = -2

Query: 1331 PRXPKKXGXPGXPGEXXXRGXPGXPPPGNXPXGXAXPXG 1215
            P  P + G PG PG    +G  G   PG    G   P G
Sbjct: 74   PGAPGRDGMPGAPGLPGSKGVKG--DPGLSMVGPPGPKG 110



 Score = 26.2 bits (55), Expect = 2.5
 Identities = 14/39 (35%), Positives = 15/39 (38%)
 Frame = -2

Query: 1331 PRXPKKXGXPGXPGEXXXRGXPGXPPPGNXPXGXAXPXG 1215
            P  P   G PG  G+    G PG P     P G   P G
Sbjct: 130  PGLPGSLGYPGEKGDLGTPGPPGYPGDVG-PKGEPGPKG 167



 Score = 25.0 bits (52), Expect = 5.8
 Identities = 19/60 (31%), Positives = 21/60 (35%), Gaps = 2/60 (3%)
 Frame = -2

Query: 1406 GKKGAXFXPXLXXLAXQIXXFAAXXPRX-PKKXGXPGXPGEXXXRG-XPGXPPPGNXPXG 1233
            G KG    P L  +A           R  P   G  G PGE   +G  P  PP    P G
Sbjct: 660  GMKGDRGMPGLEGVAGLPGMVGEKGDRGLPGMSGLNGAPGEKGQKGETPQLPPQRKGPPG 719



 Score = 24.6 bits (51), Expect = 7.6
 Identities = 13/34 (38%), Positives = 15/34 (44%), Gaps = 2/34 (5%)
 Frame = -2

Query: 1310 GXPGXPGEXXXRGXPGXPPPG--NXPXGXAXPXG 1215
            G PG  G+    G PG P P   +   G A P G
Sbjct: 382  GFPGVKGDKGTTGLPGIPGPPCVDGLPGAAGPVG 415



 Score = 24.6 bits (51), Expect = 7.6
 Identities = 12/37 (32%), Positives = 15/37 (40%), Gaps = 3/37 (8%)
 Frame = -2

Query: 1322 PKKXGXPGXPG---EXXXRGXPGXPPPGNXPXGXAXP 1221
            P++ G PG PG       +G PG   P   P     P
Sbjct: 712  PQRKGPPGPPGFNGPKGDKGLPGLAGPAGIPGAPGAP 748



 Score = 24.6 bits (51), Expect = 7.6
 Identities = 9/19 (47%), Positives = 11/19 (57%)
 Frame = +1

Query: 1291 PGXPGXPXFLGXRGXXAAK 1347
            PG PG P  +G RG   A+
Sbjct: 742  PGAPGAPGEMGLRGFEGAR 760


  Database: mosquito
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 563,979
  Number of sequences in database:  2352
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,178,889
Number of Sequences: 2352
Number of extensions: 21652
Number of successful extensions: 101
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 563,979
effective HSP length: 67
effective length of database: 406,395
effective search space used: 178813800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)