BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_H01_e584_15.seq (1573 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_4413| Best HMM Match : No HMM Matches (HMM E-Value=.) 124 1e-28 SB_59101| Best HMM Match : PT (HMM E-Value=0.026) 30 4.4 >SB_4413| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 252 Score = 124 bits (300), Expect = 1e-28 Identities = 71/132 (53%), Positives = 79/132 (59%) Frame = +1 Query: 229 WVPVTKLGRLVREGKIDKLESIYLFSLPIKXFEIIDFFLGPSLNDEVLKIMPVQKQTRAG 408 WVPVTKLGRLV++ KI LE IYLFSLPIK FEIIDFFLG +L DEVLKIMPVQKQTRAG Sbjct: 8 WVPVTKLGRLVKDLKIKTLEHIYLFSLPIKEFEIIDFFLGAALKDEVLKIMPVQKQTRAG 67 Query: 409 QRTRFKAFVCYR**QWTHWFGCEXQQGSGXCYPWXHHTRQIGCXTCXAXVYWGNKNRKAX 588 QRTRFKAFV G + + ++ YWGNK K Sbjct: 68 QRTRFKAFVAIGDSNGHVGLGVKCSKEVATAIRGAIILAKLSVIPVRRG-YWGNKIGKP- 125 Query: 589 HXCLARXXGKCG 624 H + GKCG Sbjct: 126 HTVPCKVTGKCG 137 Score = 71.7 bits (168), Expect = 1e-12 Identities = 36/52 (69%), Positives = 41/52 (78%) Frame = +2 Query: 437 AIGDNNGHIGLGVKXSKEVXXAIRGXIILAKLAVLPVPXRFIGVTKIGKPXT 592 AIGD+NGH+GLGVK SKEV AIRG IILAKL+V+PV + G KIGKP T Sbjct: 77 AIGDSNGHVGLGVKCSKEVATAIRGAIILAKLSVIPVRRGYWG-NKIGKPHT 127 >SB_59101| Best HMM Match : PT (HMM E-Value=0.026) Length = 386 Score = 30.3 bits (65), Expect = 4.4 Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 6/46 (13%) Frame = +3 Query: 144 WRQRSWRPTWSWSRTRARPW------PWQGRPERVGASHQAWSPCP 263 W+Q + +P W + ++PW PWQ P R ++ WSP P Sbjct: 316 WQQGNDQP---WQQNNSQPWQQNIVQPWQQMPTRSLSNATPWSPQP 358 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,002,898 Number of Sequences: 59808 Number of extensions: 362495 Number of successful extensions: 897 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 823 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 893 length of database: 16,821,457 effective HSP length: 85 effective length of database: 11,737,777 effective search space used: 5141146326 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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