BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_G12_e671_14.seq
(1528 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces... 181 2e-46
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 28 3.9
SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 27 5.2
SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac... 27 6.8
SPCC61.05 |||S. pombe specific multicopy membrane protein family... 27 6.8
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 27 9.0
>SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 208
Score = 181 bits (441), Expect = 2e-46
Identities = 95/199 (47%), Positives = 126/199 (63%), Gaps = 1/199 (0%)
Frame = +1
Query: 187 IPNGHFHKDWQRFVKTWFNQPARRHRRKQNRIXXXXXXXXXXXXXXLRPVVRCPTVRYHT 366
+PN HFHKDWQR+VKTWFNQP R+ RR+Q R +RP V+ PT+RY+
Sbjct: 9 LPNAHFHKDWQRYVKTWFNQPGRKLRRRQAR-QTKAAKIAPRPVEAIRPAVKPPTIRYNM 67
Query: 367 KVRAGRGFTLREIRASGLNPSFARTIGIAVDPRRRNKSVESLQINVQRLKEYRARLILFP 546
KVRAGRGFTL E++A+G++ A TIGI VD RRRN+S ESLQ NV+R+K Y A LI+FP
Sbjct: 68 KVRAGRGFTLEELKAAGVSRRVASTIGIPVDHRRRNRSEESLQRNVERIKVYLAHLIVFP 127
Query: 547 -KGKKVLKGEANEEERKLATQLRGPLMPVQQTAPKSVARPITEDEKNFKAYQYLRGARSI 723
K + KG+A + T + ++P+ Q A + A+PITE+ KNF A+ L R+
Sbjct: 128 RKAGQPKKGDATDVSGAEQTDV-AAVLPITQEAVEE-AKPITEEAKNFNAFSTLSNERAY 185
Query: 724 AKLVGIRAKRLKDAAENPD 780
A+ G RA K AE +
Sbjct: 186 ARYAGARAAFQKKRAEEAE 204
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 27.9 bits (59), Expect = 3.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -2
Query: 168 HFKRFT*NKYMKNKIATQYSF 106
HFKRFT N + +KI+ Q S+
Sbjct: 332 HFKRFTFNGFQSSKISKQISY 352
>SPBC16A3.08c |||nuclear telomere cap complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 284
Score = 27.5 bits (58), Expect = 5.2
Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 12/77 (15%)
Frame = +1
Query: 643 PKSVARPITEDEKNFKAYQYLRGARSIAKLVGIRAKRLKDAAE----NPDDV-------- 786
P P E+E +YL +S AK VG ++L++A + P+++
Sbjct: 161 PSGAQTPAAEEENVKTLDEYLSERKSAAKPVGRTVEKLENATKVEKSAPEELFASLKKSA 220
Query: 787 TKAPTAVKETKPEK*LL 837
++ +A KE+KP+K LL
Sbjct: 221 SQKKSAAKESKPKKVLL 237
>SPAC23C4.02 |crn1||actin binding protein, coronin
Crn1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 27.1 bits (57), Expect = 6.8
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +1
Query: 565 KGEANEEERKLATQLRGPLMPVQQTAPKSVARPITEDE 678
+ E N ++ + TQ + PV+++ PK + P+T E
Sbjct: 470 RDEDNHQKEETVTQPKREKTPVEKSFPKPASSPVTFSE 507
>SPCC61.05 |||S. pombe specific multicopy membrane protein family
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 469
Score = 27.1 bits (57), Expect = 6.8
Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
Frame = -2
Query: 288 LFYSILLSTMTTSWLIKPCLHKS--LPILMEMAIWYHIIPFTH 166
L S+ L +T +WL++ +S P + +A W I FTH
Sbjct: 204 LAISMFLGFITLTWLLRCIKSQSGVQPAQISLAFWVFIFVFTH 246
>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 26.6 bits (56), Expect = 9.0
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +1
Query: 682 NFKAYQYLRGARSIAKLVGIRAKRLKDAAEN 774
N ++ ++LR A S A++VG +R++ EN
Sbjct: 843 NNRSEEFLRNAASQAEIVGANKERIQKTVEN 873
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,552,873
Number of Sequences: 5004
Number of extensions: 80859
Number of successful extensions: 203
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 856299168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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