Samia BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030731E7_G10_e655_14.seq
         (1598 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P04350 Cluster: Tubulin beta-4 chain; n=4602; root|Rep:...    43   0.026
UniRef50_Q8J1D5 Cluster: Beta-tubulin; n=5; Pezizomycotina|Rep: ...    42   0.060
UniRef50_P52275 Cluster: Tubulin beta-2 chain; n=115; Bilateria|...    41   0.079
UniRef50_Q9VAX7 Cluster: CG4869-PA; n=8; Eukaryota|Rep: CG4869-P...    41   0.10 
UniRef50_UPI0000E4A877 Cluster: PREDICTED: similar to beta-tubul...    36   3.0  

>UniRef50_P04350 Cluster: Tubulin beta-4 chain; n=4602; root|Rep:
           Tubulin beta-4 chain - Homo sapiens (Human)
          Length = 444

 Score = 42.7 bits (96), Expect = 0.026
 Identities = 30/93 (32%), Positives = 36/93 (38%)
 Frame = +2

Query: 392 PXXRSXDPXDFXFGXAVXXNIWTXXHYTEXAXLXRXRLKXRSXXIXXX*LXTXDXX*HTX 571
           P  +   P +F FG +   N W   HYTE A L    L                   H+ 
Sbjct: 80  PFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSL 139

Query: 572 YGGGXAPXWAPLLXSKXREDXXDXIMXTYCVXP 670
            GGG       LL SK RE+  D IM T+ V P
Sbjct: 140 -GGGTGSGMGTLLISKIREEFPDRIMNTFSVVP 171



 Score = 37.9 bits (84), Expect = 0.74
 Identities = 24/56 (42%), Positives = 26/56 (46%)
 Frame = +1

Query: 262 DSDLQDGDASMXITXRLSGGIVRCPXPS*XDLXPXTMDSVXSGPXXQIXRPGRLXF 429
           DSDLQ    ++       G  V  P     DL P TMDSV SGP  QI RP    F
Sbjct: 39  DSDLQLERINVYYNEATGGNYV--PRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVF 92


>UniRef50_Q8J1D5 Cluster: Beta-tubulin; n=5; Pezizomycotina|Rep:
           Beta-tubulin - Nephromopsis leucostigma
          Length = 239

 Score = 41.5 bits (93), Expect = 0.060
 Identities = 30/93 (32%), Positives = 37/93 (39%)
 Frame = +2

Query: 392 PXXRSXDPXDFXFGXAVXXNIWTXXHYTEXAXLXRXRLKXRSXXIXXX*LXTXDXX*HTX 571
           P  +   P +F FG +   N W   HYTE A L    L                   H+ 
Sbjct: 31  PFXQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDQVLDVVRREAEGCDCLQGFQITHSL 90

Query: 572 YGGGXAPXWAPLLXSKXREDXXDXIMXTYCVXP 670
            GGG       LL SK RE+  D +M T+ V P
Sbjct: 91  -GGGTGAGMGTLLISKIREEFPDRMMATFSVVP 122


>UniRef50_P52275 Cluster: Tubulin beta-2 chain; n=115;
           Bilateria|Rep: Tubulin beta-2 chain - Caenorhabditis
           elegans
          Length = 450

 Score = 41.1 bits (92), Expect = 0.079
 Identities = 29/93 (31%), Positives = 36/93 (38%)
 Frame = +2

Query: 392 PXXRSXDPXDFXFGXAVXXNIWTXXHYTEXAXLXRXRLKXRSXXIXXX*LXTXDXX*HTX 571
           P  +   P +F FG +   N W   HYTE A L    L                   H+ 
Sbjct: 80  PFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSL 139

Query: 572 YGGGXAPXWAPLLXSKXREDXXDXIMXTYCVXP 670
            GGG       LL SK RE+  D IM ++ V P
Sbjct: 140 -GGGTGSGMGTLLISKIREEYPDRIMSSFSVVP 171



 Score = 34.3 bits (75), Expect = 9.1
 Identities = 15/26 (57%), Positives = 16/26 (61%)
 Frame = +1

Query: 352 DLXPXTMDSVXSGPXXQIXRPGRLXF 429
           DL P TMDSV SGP  Q+ RP    F
Sbjct: 67  DLEPGTMDSVRSGPFGQLFRPDNFVF 92


>UniRef50_Q9VAX7 Cluster: CG4869-PA; n=8; Eukaryota|Rep: CG4869-PA -
           Drosophila melanogaster (Fruit fly)
          Length = 522

 Score = 40.7 bits (91), Expect = 0.10
 Identities = 28/95 (29%), Positives = 38/95 (40%)
 Frame = +2

Query: 386 RDPXXRSXDPXDFXFGXAVXXNIWTXXHYTEXAXLXRXRLKXRSXXIXXX*LXTXDXX*H 565
           + P  +   P +F +G +   N W   HYTE A L    L+                  H
Sbjct: 78  QSPVGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELIDSVLEVLRKESEGCDCLQGFQLAH 137

Query: 566 TXYGGGXAPXWAPLLXSKXREDXXDXIMXTYCVXP 670
           +  GGG       LL SK RE+  D IM ++ V P
Sbjct: 138 SL-GGGTGSGLGTLLISKIREEYPDRIMNSFSVVP 171


>UniRef50_UPI0000E4A877 Cluster: PREDICTED: similar to beta-tubulin,
           partial; n=3; Strongylocentrotus purpuratus|Rep:
           PREDICTED: similar to beta-tubulin, partial -
           Strongylocentrotus purpuratus
          Length = 224

 Score = 35.9 bits (79), Expect = 3.0
 Identities = 23/56 (41%), Positives = 26/56 (46%)
 Frame = +1

Query: 262 DSDLQDGDASMXITXRLSGGIVRCPXPS*XDLXPXTMDSVXSGPXXQIXRPGRLXF 429
           DSDLQ    ++       G  V  P     DL P T+DSV SGP  QI RP    F
Sbjct: 10  DSDLQLERINVYYNEAAGGQYV--PRAVLLDLEPGTLDSVHSGPFGQIFRPDNFIF 63


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 586,966,622
Number of Sequences: 1657284
Number of extensions: 4887969
Number of successful extensions: 3937
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 3847
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3932
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 172603615300
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)