Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_G08_e639_14.seq
(1585 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g48330.2 68416.m05275 protein-L-isoaspartate O-methyltransfer... 118 1e-26
At3g48330.1 68416.m05274 protein-L-isoaspartate O-methyltransfer... 118 1e-26
At5g50240.1 68418.m06222 protein-L-isoaspartate O-methyltransfer... 47 4e-05
At5g14600.1 68418.m01712 expressed protein 32 0.91
At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family prot... 31 2.1
At5g52490.1 68418.m06512 fibrillarin, putative similar to fibril... 31 2.8
>At3g48330.2 68416.m05275 protein-L-isoaspartate O-methyltransferase
/ PIMT (PCM) identical to SP|Q42539
Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)
(Protein- beta-aspartate methyltransferase) (PIMT)
(Protein L-isoaspartyl methyltransferase) (L-isoaspartyl
protein carboxyl methyltransferase) {Arabidopsis
thaliana}
Length = 230
Score = 118 bits (283), Expect = 1e-26
Identities = 57/107 (53%), Positives = 72/107 (67%), Gaps = 2/107 (1%)
Frame = +2
Query: 224 NFDMIQKLKANGIVKSDAVVNAMLIADRKNYCLH--TPYLDSPQSIGYSATISAPHMHAH 397
N M++ L+ +GIV SD V AM DR + + Y+DSP SIGY+ TISAPHMHA
Sbjct: 13 NKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTISAPHMHAM 72
Query: 398 ALEILKNQLLPGEKALDXGSGSGYLTACMAXMVGETGNVIGIDHIPE 538
L++L+ L PG + LD GSG+GYLTAC A MVG G IG++HIPE
Sbjct: 73 CLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPE 119
Score = 34.7 bits (76), Expect = 0.17
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = +1
Query: 652 APYSAIHVGAAAPTMPSSSY*AXLKPG 732
APY AIHVGAAAP +P + LKPG
Sbjct: 157 APYDAIHVGAAAPEIPEALI-DQLKPG 182
>At3g48330.1 68416.m05274 protein-L-isoaspartate O-methyltransferase
/ PIMT (PCM) identical to SP|Q42539
Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)
(Protein- beta-aspartate methyltransferase) (PIMT)
(Protein L-isoaspartyl methyltransferase) (L-isoaspartyl
protein carboxyl methyltransferase) {Arabidopsis
thaliana}
Length = 230
Score = 118 bits (283), Expect = 1e-26
Identities = 57/107 (53%), Positives = 72/107 (67%), Gaps = 2/107 (1%)
Frame = +2
Query: 224 NFDMIQKLKANGIVKSDAVVNAMLIADRKNYCLH--TPYLDSPQSIGYSATISAPHMHAH 397
N M++ L+ +GIV SD V AM DR + + Y+DSP SIGY+ TISAPHMHA
Sbjct: 13 NKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTISAPHMHAM 72
Query: 398 ALEILKNQLLPGEKALDXGSGSGYLTACMAXMVGETGNVIGIDHIPE 538
L++L+ L PG + LD GSG+GYLTAC A MVG G IG++HIPE
Sbjct: 73 CLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPE 119
Score = 34.7 bits (76), Expect = 0.17
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = +1
Query: 652 APYSAIHVGAAAPTMPSSSY*AXLKPG 732
APY AIHVGAAAP +P + LKPG
Sbjct: 157 APYDAIHVGAAAPEIPEALI-DQLKPG 182
>At5g50240.1 68418.m06222 protein-L-isoaspartate
O-methyltransferase, putative / PIMT, putative similar
to SP|Q42539 Protein-L-isoaspartate O-methyltransferase
(EC 2.1.1.77) (Protein- beta-aspartate
methyltransferase) (PIMT) (Protein L-isoaspartyl
methyltransferase) (L-isoaspartyl protein carboxyl
methyltransferase) {Arabidopsis thaliana}; contains Pfam
profile PF01135: Protein-L-isoaspartate(D-aspartate)
O-methyltransferase (PCMT)
Length = 269
Score = 46.8 bits (106), Expect = 4e-05
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +2
Query: 458 GSGYLTACMAXMVGETGNVIGIDHIPE 538
G+GYLT C A MVG G V+G+DHIPE
Sbjct: 132 GTGYLTGCFALMVGAEGRVVGVDHIPE 158
Score = 30.7 bits (66), Expect = 2.8
Identities = 16/27 (59%), Positives = 17/27 (62%)
Frame = +1
Query: 652 APYSAIHVGAAAPTMPSSSY*AXLKPG 732
APY AIHVGAAA +P LKPG
Sbjct: 196 APYDAIHVGAAASEIPQPLL-DQLKPG 221
>At5g14600.1 68418.m01712 expressed protein
Length = 318
Score = 32.3 bits (70), Expect = 0.91
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = +2
Query: 419 QLLPGEKALDXGSGSGYLTACMAXMVGETGNVIGID 526
+++PG L+ G+GSG L+ +A V TG+V D
Sbjct: 105 EVVPGCVVLESGTGSGSLSTSLARAVAPTGHVYSFD 140
>At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 198
Score = 31.1 bits (67), Expect = 2.1
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +2
Query: 431 GEKALDXGSGSGYLTACMAXMVGETGNVIGIDHIPE 538
G+ LD GSG L ++ VG TG V+G+D E
Sbjct: 11 GDYVLDLCCGSGDLAFLLSEKVGSTGKVMGLDFSSE 46
>At5g52490.1 68418.m06512 fibrillarin, putative similar to
fibrillarin from {Xenopus laevis} SP|P22232, {Mus
musculus} SP|P35550, {Homo sapiens} SP|P22087
Length = 292
Score = 30.7 bits (66), Expect = 2.8
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 428 PGEKALDXGSGSGYLTACMAXMVGETGNVIGIDH 529
PG K L G+ SGY + ++ +VG G V ++H
Sbjct: 133 PGVKVLYLGASSGYTVSHVSDIVGPEGCVYAVEH 166
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,255,080
Number of Sequences: 28952
Number of extensions: 357967
Number of successful extensions: 672
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 657
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 672
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4269896256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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