Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_G07_e631_13.seq
(1589 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 182 1e-46
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 169 7e-43
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 106 1e-23
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 96 1e-20
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 85 3e-17
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 57 8e-09
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 44 8e-05
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 39 0.002
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 36 0.015
SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 29 2.4
SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 27 7.2
SPAPB18E9.03c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 27 7.2
SPBC839.03c |||neddylation protein Dcn1|Schizosaccharomyces pomb... 27 7.2
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 182 bits (443), Expect = 1e-46
Identities = 86/173 (49%), Positives = 117/173 (67%)
Frame = +1
Query: 133 TLSNGAKIPVIGIGTWKSKPGEVTQAVKDAIDIGYRHIXCAFVYGNEKEVGEAIKTKIQE 312
TL++G+KIP +G+GTW+S+P + AVK A+ GYRHI A +YGNE EVG+ IK E
Sbjct: 17 TLADGSKIPGLGLGTWRSEPNQTKNAVKTALQYGYRHIDAAAIYGNEDEVGDGIK----E 72
Query: 313 GVVKREDLFITSKLWNTFHRPDLVRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPT 492
V R+D+++TSKLW H P+ V ALE +L++L +DYLD YLIHWP ++K + FP
Sbjct: 73 SGVPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKFPK 132
Query: 493 DADGKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKP 651
D DG + + +TWKA+E L+ G VR +G+SNFN L+RIL A VKP
Sbjct: 133 DKDGNLIYEKNPIEETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAKVKP 185
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 169 bits (412), Expect = 7e-43
Identities = 84/173 (48%), Positives = 112/173 (64%)
Frame = +1
Query: 133 TLSNGAKIPVIGIGTWKSKPGEVTQAVKDAIDIGYRHIXCAFVYGNEKEVGEAIKTKIQE 312
TL NG KIP IG+GTW+S E AV A+ GYRHI A +YGNEKE+GE I+ E
Sbjct: 16 TLPNGDKIPSIGLGTWRSGKDETKNAVCAALKAGYRHIDTAHIYGNEKEIGEGIR----E 71
Query: 313 GVVKREDLFITSKLWNTFHRPDLVRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPT 492
V R D+++TSKLW HR LV ALE +L++LN++Y+D YLIHWP A P
Sbjct: 72 SGVPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEELPR 131
Query: 493 DADGKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKP 651
+ G++ + DV +TW+A+E L+ G VR +G+SNFN++ L R+L A VKP
Sbjct: 132 NEKGELIYEDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKP 184
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 106 bits (254), Expect = 1e-23
Identities = 75/177 (42%), Positives = 99/177 (55%), Gaps = 8/177 (4%)
Frame = +1
Query: 145 GAKIPV--IGIGT--WKSKPGEVTQ----AVKDAIDIGYRHIXCAFVYGNEKEVGEAIKT 300
G KIPV G+GT +K + GE+ + +VK+A+ G+ HI CA VYGNE+EVG A+K
Sbjct: 7 GPKIPVPAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAEVYGNEEEVGVALK- 65
Query: 301 KIQEGVVKREDLFITSKLWNTFHRPDLVRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDA 480
E V R LFITSK+ H D + AL SLR L DYLDLYL+H P + E
Sbjct: 66 ---EANVPRSKLFITSKV---MHNVDNIPEALNESLRKLGTDYLDLYLLHSPIPFYEK-- 117
Query: 481 IFPTDADGKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKP 651
KI S + WKA+E + GLV S+GVSNF L+ +L +++ P
Sbjct: 118 --------KIPIS-----EGWKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITP 161
Score = 32.7 bits (71), Expect = 0.14
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
Frame = +2
Query: 659 NQVECHP--YLNXKRLKQFCEDRDIMITXYSPXGXLPTD 769
NQ+E HP Y K L +FC+ + I++ Y P L D
Sbjct: 164 NQIEFHPQVYKAAKPLVEFCQSKGIIVEGYGPLSPLVRD 202
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 95.9 bits (228), Expect = 1e-20
Identities = 68/171 (39%), Positives = 87/171 (50%)
Frame = +1
Query: 115 TSVPVTTLSNGAKIPVIGIGTWKSKPGEVTQAVKDAIDIGYRHIXCAFVYGNEKEVGEAI 294
T TL+NG IP IG G + K E V A+D GYRHI A VYGNE G+AI
Sbjct: 6 TESTTVTLTNGMVIPRIGFGAFMLKYNECYGLVTQALDSGYRHIDTAAVYGNEDICGKAI 65
Query: 295 KTKIQEGVVKREDLFITSKLWNTFHRPDLVRGALEVSLRNLNIDYLDLYLIHWPQAYKED 474
++ VKR D+F+TSKL N R A+ SL +L Y+DL+LI P
Sbjct: 66 VDWCEKNNVKRTDIFLTSKLANCSDYYS-TRAAIRSSLHHLG-TYIDLFLIQSP------ 117
Query: 475 DAIFPTDADGKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRI 627
A GK + +WKA+E V G +RS+GVSN+ K LQ +
Sbjct: 118 -------AGGK-----KSRIASWKAMEEFVDSGDIRSVGVSNYGVKHLQEL 156
Score = 37.1 bits (82), Expect = 0.007
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +2
Query: 659 NQVECHPYLNXKRLKQFCEDRDIMITXYSP 748
NQ+E HP+L+ + ++C+ DI I YSP
Sbjct: 168 NQIELHPFLSQDDIVKYCQSHDIAIEAYSP 197
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 85.0 bits (201), Expect = 3e-17
Identities = 54/167 (32%), Positives = 84/167 (50%)
Frame = +1
Query: 136 LSNGAKIPVIGIGTWKSKPGEVTQAVKDAIDIGYRHIXCAFVYGNEKEVGEAIKTKIQEG 315
L+NG K P G++ + +V A+ GYRHI A +Y NE + G AI ++E
Sbjct: 8 LNNGLKCPQFAYGSYMVNRTKCFDSVYAALQCGYRHIDSAQMYHNEADCGRAILKFMEET 67
Query: 316 VVKREDLFITSKLWNTFHRPDLVRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTD 495
KRED++ TSKL N +++ S++ + Y+DL+L+H P
Sbjct: 68 GTKREDIWFTSKL-NDLSGYKSTLSSIDASVKACGLGYIDLFLLHSPYG----------- 115
Query: 496 ADGKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDN 636
D +++WKALE V G +R++GVSNF +Q +LD+
Sbjct: 116 ----------DRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDS 152
Score = 34.7 bits (76), Expect = 0.036
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +2
Query: 659 NQVECHPYLNXKRLKQFCEDRDIMITXYSP 748
NQ+E HP+ + +++ +CE + I + Y+P
Sbjct: 161 NQIELHPFCSQQKVVDYCESKGIQLAAYAP 190
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 56.8 bits (131), Expect = 8e-09
Identities = 44/143 (30%), Positives = 71/143 (49%), Gaps = 1/143 (0%)
Frame = +1
Query: 205 QAVKDAIDIGYRHIXCAFVYGNEKEVGEAIKTKIQEGV-VKREDLFITSKLWNTFHRPDL 381
Q V +A+ +G R I A Y NEKE +AI+ + V +KRED+ + +K+ ++ +
Sbjct: 28 QQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITKIPDSLQGFER 87
Query: 382 VRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDADGKIQFSDVDYVDTWKALEPL 561
A+E SLR LD+ LIH P+ +P V +++W+AL
Sbjct: 88 TWKAVEQSLRRTGRPKLDVVLIHSPK--------WP-----------VRRIESWRALLQH 128
Query: 562 VCHGLVRSLGVSNFNSKQLQRIL 630
G + +GVSN+N L+ I+
Sbjct: 129 QKEGRINKIGVSNYNIHHLEEII 151
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 43.6 bits (98), Expect = 8e-05
Identities = 19/41 (46%), Positives = 27/41 (65%)
Frame = +1
Query: 529 YVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKP 651
+VDTWK LE V G + +LGVS F +LQR++ + +V P
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVP 198
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 39.1 bits (87), Expect = 0.002
Identities = 38/131 (29%), Positives = 61/131 (46%), Gaps = 20/131 (15%)
Frame = +1
Query: 118 SVPVTTLSNGAKIPVIGIGT------WKSKPGEVTQAV-KDAIDIGYRHIXCAFVYG--- 267
S+P + N +P IG G + E QAV A D+G + +YG
Sbjct: 2 SIPTRKIGNDT-VPAIGFGCMGLHAMYGPSSEEANQAVLTHAADLGCTFWDSSDMYGFGA 60
Query: 268 NEKEVGEAIKTKIQEGVVKREDLFITSKLW----------NTFHRPDLVRGALEVSLRNL 417
NE+ +G K Q G +R+++F+ +K + + PD + AL++SL+ L
Sbjct: 61 NEECIGRWFK---QTG--RRKEIFLATKFGYEKNPETGELSLNNEPDYIEKALDLSLKRL 115
Query: 418 NIDYLDLYLIH 450
ID +DLY +H
Sbjct: 116 GIDCIDLYYVH 126
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 35.9 bits (79), Expect = 0.015
Identities = 37/129 (28%), Positives = 57/129 (44%), Gaps = 16/129 (12%)
Frame = +1
Query: 118 SVPVTTLS-NGAKIPVIGIGTWKSKPGE------VTQAVKDAIDIGYRHIXCAFVYGN-- 270
+VP L +G K+ +G W + E +K A D+G A +Y N
Sbjct: 12 NVPFRFLGRSGLKVSAFSLGGWLTYGNEGYDVEHTKNCLKQAWDLGINTFDTAEIYSNGN 71
Query: 271 -EKEVGEAIKT---KIQEGVVKREDLF-ITSKLWNT--FHRPDLVRGALEVSLRNLNIDY 429
E +G+AIK E V+ + F +KL NT R ++ G L SL+ L + Y
Sbjct: 72 SETVMGKAIKELGWDRSEYVITTKVFFGAGTKLPNTTGLSRKHIIEG-LNASLKRLGLPY 130
Query: 430 LDLYLIHWP 456
+D+ + H P
Sbjct: 131 VDVIMAHRP 139
>SPBC543.08 |||phosphoinositide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 250
Score = 28.7 bits (61), Expect = 2.4
Identities = 15/55 (27%), Positives = 27/55 (49%)
Frame = -3
Query: 807 ILHQMRVTRLSPGSVGXXPXGEYXVIIISRSSQNCFNRXWFKYGWHSTWLVTWFH 643
+L ++ T L GS+ ++ + +S+N N + K GW T LV ++H
Sbjct: 25 LLFEIYATTLLLGSIYSIYVDKWSITSYFGNSKNLINLIFVKRGWFWTSLVYFYH 79
>SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr
3|||Manual
Length = 872
Score = 27.1 bits (57), Expect = 7.2
Identities = 7/19 (36%), Positives = 15/19 (78%)
Frame = +1
Query: 505 KIQFSDVDYVDTWKALEPL 561
+++F+ +DY+ W+AL P+
Sbjct: 164 RVEFARLDYIKVWRALAPI 182
>SPAPB18E9.03c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 44
Score = 27.1 bits (57), Expect = 7.2
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 199 LLLVSTSKCLFQLREFLHRCSKL 131
LL +S S+C+F F H C+K+
Sbjct: 19 LLQISISRCVFSKNGFYHVCNKM 41
>SPBC839.03c |||neddylation protein Dcn1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 27.1 bits (57), Expect = 7.2
Identities = 16/49 (32%), Positives = 24/49 (48%)
Frame = +1
Query: 337 FITSKLWNTFHRPDLVRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAI 483
F+ S FHR V GAL +S +L D L L + Q ++ D ++
Sbjct: 97 FLKSPRMGEFHRESFVEGALNLSTTSL--DQLKLAIKEKVQVWRSDASL 143
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,080,493
Number of Sequences: 5004
Number of extensions: 73312
Number of successful extensions: 212
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 897924822
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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