Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_G04_e607_14.seq
(1550 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P60468 Cluster: Protein transport protein Sec61 subunit... 73 3e-11
UniRef50_Q6DGH3 Cluster: Zgc:92922; n=13; Euteleostomi|Rep: Zgc:... 69 4e-10
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L... 67 1e-09
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet... 65 4e-09
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo... 64 7e-09
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ... 62 3e-08
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ... 62 4e-08
UniRef50_Q5BSB6 Cluster: SJCHGC05179 protein; n=3; Bilateria|Rep... 50 1e-04
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba... 48 9e-04
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:... 45 0.005
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ... 41 0.10
UniRef50_P40602 Cluster: Anter-specific proline-rich protein APG... 39 0.31
UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;... 34 8.8
>UniRef50_P60468 Cluster: Protein transport protein Sec61 subunit
beta; n=31; Eukaryota|Rep: Protein transport protein
Sec61 subunit beta - Homo sapiens (Human)
Length = 96
Score = 72.5 bits (170), Expect = 3e-11
Identities = 37/71 (52%), Positives = 40/71 (56%)
Frame = +1
Query: 280 ASGSTVRQRKXXXXXXXXXXXXXXXXXXXMWRFYTDDSXXXXXXXXXXXXMSLLFIASVF 459
A+GSTVRQRK MWRFYT+DS MSLLFIASVF
Sbjct: 26 AAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVF 85
Query: 460 MLHIWGKYTRA 492
MLHIWGKYTR+
Sbjct: 86 MLHIWGKYTRS 96
>UniRef50_Q6DGH3 Cluster: Zgc:92922; n=13; Euteleostomi|Rep:
Zgc:92922 - Danio rerio (Zebrafish) (Brachydanio rerio)
Length = 97
Score = 68.5 bits (160), Expect = 4e-10
Identities = 39/78 (50%), Positives = 43/78 (55%), Gaps = 1/78 (1%)
Frame = +1
Query: 262 TSAPRTASGSTVRQRKXXXXXXXXXXXXXXXXXXX-MWRFYTDDSXXXXXXXXXXXXMSL 438
T APRTA G++ RQRK MWRFYT+DS MSL
Sbjct: 21 TVAPRTA-GTSARQRKATSSSARSGGRSTASAGTGGMWRFYTEDSPGLKVGPVPVLVMSL 79
Query: 439 LFIASVFMLHIWGKYTRA 492
LFIASVFMLHIWGKYTR+
Sbjct: 80 LFIASVFMLHIWGKYTRS 97
>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
protein - Phage M13mp18
Length = 102
Score = 67.3 bits (157), Expect = 1e-09
Identities = 31/54 (57%), Positives = 36/54 (66%)
Frame = +3
Query: 648 NSRGGPGTQFRPIVSRITISLAVVLQRRDWGNPGVTHLNRLAAHPLXASWRXAK 809
NS PG +++LAVVLQRRDW NPGVT LNRLAAHP ASWR ++
Sbjct: 6 NSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSE 59
>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
Beta-galactosidase - Escherichia coli (strain K12)
Length = 1024
Score = 65.3 bits (152), Expect = 4e-09
Identities = 30/41 (73%), Positives = 34/41 (82%)
Frame = +3
Query: 687 VSRITISLAVVLQRRDWGNPGVTHLNRLAAHPLXASWRXAK 809
++ IT SLAVVLQRRDW NPGVT LNRLAAHP ASWR ++
Sbjct: 1 MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSE 41
>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
Eukaryota|Rep: beta-galactosidase - Entamoeba
histolytica HM-1:IMSS
Length = 86
Score = 64.5 bits (150), Expect = 7e-09
Identities = 27/32 (84%), Positives = 29/32 (90%)
Frame = +1
Query: 703 FHWPSFYNVVTGETLALPTLIALQHIPFXPAG 798
+HWPSFYNVVTG+TLALP LIALQHIP PAG
Sbjct: 4 WHWPSFYNVVTGKTLALPNLIALQHIPLSPAG 35
>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
organisms|Rep: LacZ-alpha peptide - Escherichia coli
Length = 90
Score = 62.5 bits (145), Expect = 3e-08
Identities = 28/35 (80%), Positives = 30/35 (85%)
Frame = +3
Query: 705 SLAVVLQRRDWGNPGVTHLNRLAAHPLXASWRXAK 809
SLAVVLQRRDW NPGVT LNRLAAHP ASWR ++
Sbjct: 21 SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSE 55
>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
Erwinia amylovora|Rep: Putative uncharacterized protein
- Erwinia amylovora (Fire blight bacteria)
Length = 123
Score = 62.1 bits (144), Expect = 4e-08
Identities = 31/51 (60%), Positives = 37/51 (72%), Gaps = 3/51 (5%)
Frame = +3
Query: 666 GTQFRPIVSRITIS---LAVVLQRRDWGNPGVTHLNRLAAHPLXASWRXAK 809
G + P+V ++ S LAVVLQRRDW NPGVT LNRLAAHP ASWR ++
Sbjct: 51 GELWYPLVKALSESYYGLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSE 101
>UniRef50_Q5BSB6 Cluster: SJCHGC05179 protein; n=3; Bilateria|Rep:
SJCHGC05179 protein - Schistosoma japonicum (Blood
fluke)
Length = 88
Score = 50.4 bits (115), Expect = 1e-04
Identities = 30/78 (38%), Positives = 38/78 (48%)
Frame = +1
Query: 259 ATSAPRTASGSTVRQRKXXXXXXXXXXXXXXXXXXXMWRFYTDDSXXXXXXXXXXXXMSL 438
AT++PR SG RQRK ++ FY++DS MSL
Sbjct: 12 ATASPR-GSGGGARQRKAPAASARRPVAPTAQKNP-VFLFYSEDSPGIKVGPVPVLVMSL 69
Query: 439 LFIASVFMLHIWGKYTRA 492
FI SVF+LH WGKYTR+
Sbjct: 70 CFIVSVFLLHFWGKYTRS 87
>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
pneumoniae
Length = 1034
Score = 47.6 bits (108), Expect = 9e-04
Identities = 23/41 (56%), Positives = 27/41 (65%)
Frame = +3
Query: 717 VLQRRDWGNPGVTHLNRLAAHPLXASWRXAKXPAPIXLPNS 839
VL R DW N +THLNRL AHP+ ASWR + A LP+S
Sbjct: 17 VLAREDWHNQTITHLNRLPAHPVFASWRD-ELAARDNLPSS 56
>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
Beta-galactosidase - Yersinia pseudotuberculosis
Length = 1066
Score = 45.2 bits (102), Expect = 0.005
Identities = 18/36 (50%), Positives = 24/36 (66%)
Frame = +3
Query: 693 RITISLAVVLQRRDWGNPGVTHLNRLAAHPLXASWR 800
++ +SL +L RRDW NP +T +RL AHP SWR
Sbjct: 10 QVQLSLPQILSRRDWENPQITQYHRLEAHPPFHSWR 45
>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
Enterobacter sakazakii ATCC BAA-894|Rep: Putative
uncharacterized protein - Enterobacter sakazakii ATCC
BAA-894
Length = 1043
Score = 40.7 bits (91), Expect = 0.10
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = +3
Query: 699 TISLAVVLQRRDWGNPGVTHLNRLAAHPLXASWRXA 806
T LA +L R DW NP +T +NRL +H WR A
Sbjct: 15 TRPLATILARNDWQNPAITSVNRLPSHTPLHGWRDA 50
>UniRef50_P40602 Cluster: Anter-specific proline-rich protein APG
precursor; n=4; Brassicaceae|Rep: Anter-specific
proline-rich protein APG precursor - Arabidopsis thaliana
(Mouse-ear cress)
Length = 534
Score = 39.1 bits (87), Expect = 0.31
Identities = 35/143 (24%), Positives = 42/143 (29%)
Frame = +1
Query: 1033 PXPXRKPXXXQGPTXXPXXFGXGPKXXWT*GXPINXSGXPXSKXGKKXXPXAGXKALPQP 1212
P P +P PT P PK P S P + G P K P+P
Sbjct: 49 PRPYPQPWPMNPPTPDP-----SPKPV----APPGPSSKPVAPPGPSPCPSPPPKPQPKP 99
Query: 1213 PPXXXPWPXXXXEXXPXCFXLXXPXXPXRXXGXATSPSFXFPXXPRXXXWAXPXXXXXPX 1392
PP P P P + P P P+ P P+ P P
Sbjct: 100 PPAPSPSPCPSPPPKPQPKPVPPPACPPTPPKPQPKPA--PPPEPKPAPPPAPKPVPCPS 157
Query: 1393 XPXLXSPXXXXAPIXXXPPVPXP 1461
P +P P PP P P
Sbjct: 158 PPKPPAPTPKPVPPHGPPPKPAP 180
>UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;
n=1; Danio rerio|Rep: PREDICTED: hypothetical protein -
Danio rerio
Length = 195
Score = 34.3 bits (75), Expect = 8.8
Identities = 15/18 (83%), Positives = 15/18 (83%)
Frame = +3
Query: 705 SLAVVLQRRDWGNPGVTH 758
SLAVVLQRRDW NP TH
Sbjct: 178 SLAVVLQRRDWENPMKTH 195
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,074,042,409
Number of Sequences: 1657284
Number of extensions: 18350116
Number of successful extensions: 63234
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 40221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 55710
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 166151143700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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