BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_G04_e607_14.seq (1550 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60460.1 68418.m07583 sec61beta family protein similar to SP|... 34 0.22 At2g45070.1 68415.m05610 sec61beta family protein similar to SP|... 34 0.22 At3g60540.2 68416.m06772 sec61beta family protein similar to SP|... 32 1.2 At3g60540.1 68416.m06771 sec61beta family protein similar to SP|... 32 1.2 At4g18570.1 68417.m02749 proline-rich family protein common fami... 30 3.6 At1g20130.1 68414.m02518 family II extracellular lipase, putativ... 30 3.6 At4g30460.1 68417.m04325 glycine-rich protein 30 4.7 At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 30 4.7 >At5g60460.1 68418.m07583 sec61beta family protein similar to SP|P52870 Protein transport protein SEC61 beta 1 subunit {Saccharomyces cerevisiae}; contains Pfam profile PF03911: Sec61beta family Length = 109 Score = 34.3 bits (75), Expect = 0.22 Identities = 17/38 (44%), Positives = 20/38 (52%) Frame = +1 Query: 367 MWRFYTDDSXXXXXXXXXXXXMSLLFIASVFMLHIWGK 480 M RFYTDD+ MSL FI V LH++GK Sbjct: 62 MLRFYTDDAPGLKISPTVVLIMSLCFIGFVTALHVFGK 99 >At2g45070.1 68415.m05610 sec61beta family protein similar to SP|P52870 Protein transport protein SEC61 beta 1 subunit {Saccharomyces cerevisiae}; contains Pfam profile PF03911: Sec61beta family Length = 82 Score = 34.3 bits (75), Expect = 0.22 Identities = 21/74 (28%), Positives = 31/74 (41%), Gaps = 1/74 (1%) Frame = +1 Query: 262 TSAP-RTASGSTVRQRKXXXXXXXXXXXXXXXXXXXMWRFYTDDSXXXXXXXXXXXXMSL 438 + AP R ++ +T R+ M +FYTDD+ MS+ Sbjct: 4 SGAPQRGSAAATASMRRRKPTSGAGGGGASGGAAGSMLQFYTDDAPGLKISPNVVLIMSI 63 Query: 439 LFIASVFMLHIWGK 480 FIA V +LH+ GK Sbjct: 64 GFIAFVAVLHVMGK 77 >At3g60540.2 68416.m06772 sec61beta family protein similar to SP|P52870 Protein transport protein SEC61 beta 1 subunit {Saccharomyces cerevisiae}; contains Pfam profile PF03911: Sec61beta family Length = 81 Score = 31.9 bits (69), Expect = 1.2 Identities = 16/38 (42%), Positives = 21/38 (55%) Frame = +1 Query: 367 MWRFYTDDSXXXXXXXXXXXXMSLLFIASVFMLHIWGK 480 M +FYTDD+ MS+ FIA V +LH+ GK Sbjct: 37 MLQFYTDDAPGLKISPNVVLIMSIGFIAFVAVLHVMGK 74 >At3g60540.1 68416.m06771 sec61beta family protein similar to SP|P52870 Protein transport protein SEC61 beta 1 subunit {Saccharomyces cerevisiae}; contains Pfam profile PF03911: Sec61beta family Length = 81 Score = 31.9 bits (69), Expect = 1.2 Identities = 16/38 (42%), Positives = 21/38 (55%) Frame = +1 Query: 367 MWRFYTDDSXXXXXXXXXXXXMSLLFIASVFMLHIWGK 480 M +FYTDD+ MS+ FIA V +LH+ GK Sbjct: 37 MLQFYTDDAPGLKISPNVVLIMSIGFIAFVAVLHVMGK 74 >At4g18570.1 68417.m02749 proline-rich family protein common family members: At3g25690, At4g04980, At5g61090 [Arabidopsis thaliana] Length = 642 Score = 30.3 bits (65), Expect = 3.6 Identities = 14/36 (38%), Positives = 15/36 (41%), Gaps = 1/36 (2%) Frame = +2 Query: 1157 PKXEK-KXXPXPXXRPXPNPPPXXPPGXGXXGNXXP 1261 P +K K P P P P PPP PP G P Sbjct: 16 PSTKKTKDMPSPLPLPPPPPPPLKPPSSGSATTKPP 51 >At1g20130.1 68414.m02518 family II extracellular lipase, putative contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566) Length = 1006 Score = 30.3 bits (65), Expect = 3.6 Identities = 27/111 (24%), Positives = 32/111 (28%) Frame = +1 Query: 1129 PINXSGXPXSKXGKKXXPXAGXKALPQPPPXXXPWPXXXXEXXPXCFXLXXPXXPXRXXG 1308 P S P + G P K P+PPP P P P + P P Sbjct: 15 PPGPSSKPVAPPGPSPCPSPPPKPQPKPPPAPSPSPCPSPPPKPQPKPVPPPACP--PTP 72 Query: 1309 XATSPSFXFPXXPRXXXWAXPXXXXXPXXPXLXSPXXXXAPIXXXPPVPXP 1461 P P P+ P P P +P P PP P P Sbjct: 73 PKPQPKPAPPPEPKPAPPPAPKPVPCPSPPKPPAPTPKPVPPHGPPPKPAP 123 >At4g30460.1 68417.m04325 glycine-rich protein Length = 162 Score = 29.9 bits (64), Expect = 4.7 Identities = 22/92 (23%), Positives = 25/92 (27%), Gaps = 2/92 (2%) Frame = -1 Query: 1448 GGXXXXGAXXXXGEXKXGXXGXXXXXGXAXXXXRGXXGNX--KEGEVAXPXXRXGXXGXX 1275 GG GA G G + G G+ + G A G G Sbjct: 52 GGGSGSGAGAGSGSGGGGSSSSSSSSSSSSSSSGGGGGDAGSEAGSYAGSHAGSGSGGRS 111 Query: 1274 X*KQXGXXSXXXXGQGXXXGGGWGRAXXPAXG 1179 G S G G GGG GR G Sbjct: 112 G-SGRGRGSGGGGGHGGGGGGGGGRGGGGGSG 142 >At3g19020.1 68416.m02415 leucine-rich repeat family protein / extensin family protein similar to extensin-like protein [Lycopersicon esculentum] gi|5917664|gb|AAD55979; contains leucine-rich repeats, Pfam:PF00560; contains proline rich extensin domains, INTERPRO:IPR002965 Length = 956 Score = 29.9 bits (64), Expect = 4.7 Identities = 13/41 (31%), Positives = 16/41 (39%) Frame = +2 Query: 1109 PNGPKEXXLTXREXXXPKXEKKXXPXPXXRPXPNPPPXXPP 1231 P PK+ E P+ K+ P P P P PP P Sbjct: 536 PQPPKQETPKPEESPKPQPPKQETPKPEESPKPQPPKQETP 576 Score = 29.9 bits (64), Expect = 4.7 Identities = 13/41 (31%), Positives = 16/41 (39%) Frame = +2 Query: 1109 PNGPKEXXLTXREXXXPKXEKKXXPXPXXRPXPNPPPXXPP 1231 P PK+ E P+ K+ P P P P PP P Sbjct: 552 PQPPKQETPKPEESPKPQPPKQETPKPEESPKPQPPKQEQP 592 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 23,445,428 Number of Sequences: 28952 Number of extensions: 416112 Number of successful extensions: 1590 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 988 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1473 length of database: 12,070,560 effective HSP length: 84 effective length of database: 9,638,592 effective search space used: 4163871744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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