Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_G03_e599_13.seq
(1545 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 32 0.18
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 30 0.98
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 29 1.7
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 29 2.3
SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo... 28 4.0
SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 5.2
SPCC285.17 |spp27|uaf30|RNA polymerase I upstream activation fac... 27 5.2
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 32.3 bits (70), Expect = 0.18
Identities = 17/70 (24%), Positives = 24/70 (34%)
Frame = +2
Query: 1316 FVLXSPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPX 1495
++ SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1571 YMPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPT 1630
Query: 1496 LLLXSSXTPS 1525
S +PS
Sbjct: 1631 SPSYSPTSPS 1640
Score = 31.9 bits (69), Expect = 0.24
Identities = 17/66 (25%), Positives = 23/66 (34%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ ++ P P P P P
Sbjct: 1582 SPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1641
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1642 SPTSPS 1647
Score = 31.9 bits (69), Expect = 0.24
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1603 SPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1662
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1663 SPTSPS 1668
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1610 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1669
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1670 SPTSPS 1675
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1617 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1676
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1677 SPTSPS 1682
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1624 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1683
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1684 SPTSPS 1689
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1631 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1690
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1691 SPTSPS 1696
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1638 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1697
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1698 SPTSPS 1703
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1645 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1704
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1705 SPTSPS 1710
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1652 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1711
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1712 SPTSPS 1717
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1659 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1718
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1719 SPTSPS 1724
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1666 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1725
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1726 SPTSPS 1731
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1673 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1732
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1733 SPTSPS 1738
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1680 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1739
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1740 SPTSPS 1745
Score = 31.1 bits (67), Expect = 0.43
Identities = 17/66 (25%), Positives = 22/66 (33%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ SP+ + P P P P P
Sbjct: 1687 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1746
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1747 SPTSPS 1752
Score = 27.9 bits (59), Expect = 4.0
Identities = 16/66 (24%), Positives = 21/66 (31%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y P+ S + + P P P P P
Sbjct: 1589 SPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1648
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1649 SPTSPS 1654
Score = 27.9 bits (59), Expect = 4.0
Identities = 16/66 (24%), Positives = 21/66 (31%)
Frame = +2
Query: 1328 SPSFXXXSPFYXPSFSXXSPSXXFXXSSXPXXXXXXIXXXPXXPXPLPPRXXXPPXLLLX 1507
SPS+ SP Y + SP+ + P P P P P
Sbjct: 1596 SPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1655
Query: 1508 SSXTPS 1525
S +PS
Sbjct: 1656 SPTSPS 1661
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.9 bits (64), Expect = 0.98
Identities = 20/72 (27%), Positives = 36/72 (50%)
Frame = +2
Query: 173 ELEERRLSSPRKKELEKRGLSNLRKGERTIIKHAEEGAVEERGIKLAEGTGRKRTIKPAE 352
E+ E R+ + ++KE EK+ LRK ++ K +G+ E + +E + PAE
Sbjct: 171 EIPEVRVKTKKEKEREKKEREKLRKKQQQAKK---KGSTGEDTLASSEVSSEVDISTPAE 227
Query: 353 DELKKEDYQACG 388
++ + QA G
Sbjct: 228 NDSSAKGKQAAG 239
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 29.1 bits (62), Expect = 1.7
Identities = 23/104 (22%), Positives = 43/104 (41%)
Frame = +2
Query: 152 AKKNTTKELEERRLSSPRKKELEKRGLSNLRKGERTIIKHAEEGAVEERGIKLAEGTGRK 331
A++N +E EE+ +K + R+ E + AEE A E K A+ +
Sbjct: 597 AEENAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEK-AKREAEE 655
Query: 332 RTIKPAEDELKKEDYQACGR*MTTVEEDDCNRRKKKTTSLEERR 463
+ + AE+ K+E + R + + + K+ T +R
Sbjct: 656 KAKREAEENAKREAEEKAKREAEENAKREAEEKVKRETEENAKR 699
Score = 28.7 bits (61), Expect = 2.3
Identities = 25/107 (23%), Positives = 47/107 (43%), Gaps = 3/107 (2%)
Frame = +2
Query: 152 AKKNTTKELEE---RRLSSPRKKELEKRGLSNLRKGERTIIKHAEEGAVEERGIKLAEGT 322
A++N +E EE R K+E E++ R+ E + AEE A E K A+
Sbjct: 557 AEENAKREAEEQAKREAEEKAKREAEEKA---KREAEEKAKREAEENAKREAEEK-AKRE 612
Query: 323 GRKRTIKPAEDELKKEDYQACGR*MTTVEEDDCNRRKKKTTSLEERR 463
++ + AE++ K+E + R + + + K+ + +R
Sbjct: 613 AEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKR 659
Score = 26.6 bits (56), Expect = 9.2
Identities = 19/73 (26%), Positives = 33/73 (45%)
Frame = +2
Query: 152 AKKNTTKELEERRLSSPRKKELEKRGLSNLRKGERTIIKHAEEGAVEERGIKLAEGTGRK 331
A++ +E EE+ +K + + R+ E + AEE A E K+ T +
Sbjct: 637 AEEKAKREAEEKAKREAEEKAKREAEENAKREAEEKAKREAEENAKREAEEKVKRET-EE 695
Query: 332 RTIKPAEDELKKE 370
+ AE+E K+E
Sbjct: 696 NAKRKAEEEGKRE 708
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 28.7 bits (61), Expect = 2.3
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +2
Query: 155 KKNTTKELEERRLSSPRKKELEKRGLSNLRKGER 256
K+ T KE+E+ + + KKE+++R L ++ E+
Sbjct: 288 KRYTRKEMEQMKRRTKEKKEMKRRALYDIASDEK 321
>SPBC1539.10 |||ribosome biogenesis protein Nop16
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 209
Score = 27.9 bits (59), Expect = 4.0
Identities = 22/83 (26%), Positives = 35/83 (42%)
Frame = +3
Query: 24 HSKXKKLFMVCWPNDCTAAVSCEVYGSVEERKIAYLEKKTSMSRRKILPRSWKKEDYQAR 203
H + K L V A EV +EE + KK + K+LP S + Y R
Sbjct: 114 HGEAKTLDDVLDKEISIAPAKTEVVRQLEEEAV----KKAARQSNKMLPLSAFEHAYIQR 169
Query: 204 GRRSWRKEDYQTCGKEKGLSSSM 272
+ ED+++ K+ L+S +
Sbjct: 170 LINKYGTEDFESMAKDVKLNSKL 192
>SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 395
Score = 27.5 bits (58), Expect = 5.2
Identities = 29/108 (26%), Positives = 52/108 (48%), Gaps = 7/108 (6%)
Frame = +2
Query: 155 KKNTTKELEERRLSSPRKKELEK--RGLSNLRKGERTIIKHAEEG----AVEERGIKL-A 313
KKN E+ R SS K ELEK + L +L + + IK +E + +K A
Sbjct: 129 KKNVETEVTSRSTSSQEKDELEKQVKTLHDLNEQKDKKIKELKERINDLTYDYETLKANA 188
Query: 314 EGTGRKRTIKPAEDELKKEDYQACGR*MTTVEEDDCNRRKKKTTSLEE 457
+ + K+T+ ++ E E++Q+ + E++ N+R+ K E+
Sbjct: 189 DDSEGKQTL-VSKREAALEEFQS----KLLIRENEINKRELKMNGKED 231
>SPCC285.17 |spp27|uaf30|RNA polymerase I upstream activation factor
complex subunit Spp27|Schizosaccharomyces pombe|chr
3|||Manual
Length = 233
Score = 27.5 bits (58), Expect = 5.2
Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 6/64 (9%)
Frame = +2
Query: 194 SSPRKKE-LEKRGLSNLRKGERTIIKHAEEG-----AVEERGIKLAEGTGRKRTIKPAED 355
++P +KE LEK+ + KG+R EEG +R EG RKR PA +
Sbjct: 56 AAPSEKESLEKQAPARKTKGKRATENGTEEGKKPAKRTRKRKEDGEEGGKRKRNQDPANN 115
Query: 356 ELKK 367
L K
Sbjct: 116 PLNK 119
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,036,450
Number of Sequences: 5004
Number of extensions: 66978
Number of successful extensions: 233
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 868192212
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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