Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030731E7_G01_e583_13.seq
         (1474 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC16C4.03 |pin1||peptidyl-prolyl cis-trans isomerase Pin1|Schi...   120   4e-28
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    37   0.008
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    30   0.70 
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi...    30   0.70 
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    30   0.93 
SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-...    27   6.6  

>SPCC16C4.03 |pin1||peptidyl-prolyl cis-trans isomerase
           Pin1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 175

 Score =  120 bits (290), Expect = 4e-28
 Identities = 69/170 (40%), Positives = 99/170 (58%), Gaps = 13/170 (7%)
 Frame = +2

Query: 155 LPEGWQARKSRSTGMTYYLNKYTKQSQWEKPGSP--AAV-----NEDEDN------SSSK 295
           LP+ W  + SRS    Y+ N  T +S WE P +   AA+     NE +++      S+S 
Sbjct: 6   LPKPWIVKISRSRNRPYFFNTETHESLWEPPAATDMAALKKFIANELQESVTPTEASNSP 65

Query: 296 EVQCSHILVKHKXXXXXXXXXXXXITRTKEEAIQIIKGYRTEIVNREVGFDEVARLYSDC 475
           +++ SH+LVKH+            ITR+KEEA ++ + Y   + +  V   ++A   SDC
Sbjct: 66  KIRASHLLVKHRESRRPSSWKEEHITRSKEEARKLAEHYEQLLKSGSVSMHDLAMKESDC 125

Query: 476 SSAKRDGDLGRFKKGQMQKPFEDVAFTLKIGQLSQLVETQSGVHIILRTA 625
           SSA+R G+LG F + +MQKPFED AF LK G++S +VET SG HII R A
Sbjct: 126 SSARRGGELGEFGRDEMQKPFEDAAFALKPGEISGVVETSSGFHIIQRHA 175


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 36.7 bits (81), Expect = 0.008
 Identities = 18/52 (34%), Positives = 29/52 (55%)
 Frame = +2

Query: 143 QEDSLPEGWQARKSRSTGMTYYLNKYTKQSQWEKPGSPAAVNEDEDNSSSKE 298
           Q   LP GW+ R++ S G TYY++  T+ + W +P S  + N   + SS  +
Sbjct: 234 QYGRLPPGWE-RRADSLGRTYYVDHNTRTTTWTRPAS--STNPVHNTSSDSQ 282



 Score = 36.3 bits (80), Expect = 0.011
 Identities = 14/40 (35%), Positives = 26/40 (65%)
 Frame = +2

Query: 155 LPEGWQARKSRSTGMTYYLNKYTKQSQWEKPGSPAAVNED 274
           LP GW+ R + S  + Y+++  TK + W+ P  P+A+++D
Sbjct: 366 LPSGWEMRLTNSARV-YFVDHNTKTTTWDDPRLPSALDQD 404


>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 273

 Score = 30.3 bits (65), Expect = 0.70
 Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = +2

Query: 149 DSLPEGWQARKSRSTGMTYYLNKYTK--QSQWEKP 247
           + LP GW A+     G  +Y+N+  +  Q QWE P
Sbjct: 8   EGLPSGWVAQWDAEYGTYFYVNESAQNPQPQWEPP 42


>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
           Usp104|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 695

 Score = 30.3 bits (65), Expect = 0.70
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = +2

Query: 134 AAAQEDSLPEGWQARKSRSTGMTYYLNKYTKQSQWEKP 247
           +AA   ++   W   K+  + + YY N  T++S WEKP
Sbjct: 25  SAAPSKTVASDWHEVKTEDSRV-YYYNSVTRKSVWEKP 61


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 29.9 bits (64), Expect = 0.93
 Identities = 14/56 (25%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
 Frame = +2

Query: 128 EMAAAQEDSLPEGWQARKSRSTGMTYYLNKYTKQSQWEKPGSPAAVNED-EDNSSS 292
           +  A ++  LP GW+ R S    + Y+++  TK + W  P      ++   +N+ S
Sbjct: 235 QQVAVEKGPLPAGWEMRLSEDYHV-YFVDHSTKTTTWSDPRDNVVASDSVSENTDS 289


>SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase
           [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 27.1 bits (57), Expect = 6.6
 Identities = 13/44 (29%), Positives = 24/44 (54%)
 Frame = +2

Query: 98  TILLNDPRKSEMAAAQEDSLPEGWQARKSRSTGMTYYLNKYTKQ 229
           +I++N     E+A +  D+L    + R++    +  Y+NKYT Q
Sbjct: 428 SIVINPWNTEELANSIHDALTMPEKQREANENKLFRYVNKYTSQ 471


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,284,428
Number of Sequences: 5004
Number of extensions: 77631
Number of successful extensions: 178
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 820620036
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)