BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_F12_e670_12.seq (1512 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 33 0.078 SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosa... 33 0.14 SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 32 0.18 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 31 0.55 SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyce... 31 0.55 SPBC1604.09c |||exoribonuclease Rex4 |Schizosaccharomyces pombe|... 29 1.3 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 3.9 SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 28 3.9 SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 27 5.1 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 27 8.9 SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyc... 27 8.9 >SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 33.5 bits (73), Expect = 0.078 Identities = 18/51 (35%), Positives = 30/51 (58%), Gaps = 3/51 (5%) Frame = +1 Query: 634 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDESRE---NDR 777 RD+HH+ S+ +R R+ N+ S D + HS ++ ++ R+ RE NDR Sbjct: 173 RDRHHR-SNQDRSRERSDNEQHSSDKREHS--RRSYRNDRNNWRERTHNDR 220 Score = 31.9 bits (69), Expect = 0.24 Identities = 14/50 (28%), Positives = 28/50 (56%) Frame = +1 Query: 634 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDESRENDRSS 783 +++ H+ HH ++ +S D+++HS D++ H R S NDR++ Sbjct: 168 KERRHRDRHHRSNQDRSRERS---DNEQHSSDKREHSR---RSYRNDRNN 211 >SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosaccharomyces pombe|chr 1|||Manual Length = 639 Score = 32.7 bits (71), Expect = 0.14 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 2/39 (5%) Frame = +1 Query: 658 HHERDRKSEHNKS--SSKDSKRHSGDQKXHKRARDESRE 768 HH R R+S+H + S + S R +GD+ H+R E E Sbjct: 31 HHSRHRESKHGRHDRSERPSSREAGDRSRHERFSKEPLE 69 >SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 604 Score = 32.3 bits (70), Expect = 0.18 Identities = 14/48 (29%), Positives = 24/48 (50%) Frame = +1 Query: 640 KHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDESRENDRSS 783 +H +S +R S +++ D + D + H+R R SR +RSS Sbjct: 183 RHSRSRRRYEERSSRSSRAHDYDYEDLRDDDRSHERKRSRSRPRERSS 230 Score = 28.7 bits (61), Expect = 2.2 Identities = 14/46 (30%), Positives = 20/46 (43%) Frame = +1 Query: 646 HKSSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDESRENDRSS 783 H++ H R S + S+ HS R+ SR+ DRSS Sbjct: 110 HRTPRHHRRSYSPRSDYGSRSPSPHSSVDSHQSRSPVRSRDRDRSS 155 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 30.7 bits (66), Expect = 0.55 Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 2/43 (4%) Frame = +1 Query: 637 DKHHKSSHHERDRKSEHNKSSSKDSKRHSG--DQKXHKRARDE 759 ++H HH EH+K KD + H G D++ HK +D+ Sbjct: 348 EEHEGPEHHRGPEDKEHHKGP-KDKEHHKGPKDKEHHKGPKDK 389 Score = 29.5 bits (63), Expect = 1.3 Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 7/52 (13%) Frame = +1 Query: 634 RDK-HHKS----SHHERDRKSEHNKSSSKDSKRHSG--DQKXHKRARDESRE 768 +DK HHK HH+ + EH+K KD + H G D++ H+ +++ E Sbjct: 378 KDKEHHKGPKDKEHHKGPKDKEHHKGP-KDKEHHKGPKDKEHHQGPKEKHNE 428 Score = 28.7 bits (61), Expect = 2.2 Identities = 15/45 (33%), Positives = 19/45 (42%) Frame = +1 Query: 634 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDESRE 768 + KHHK H + K H K DS ++ H DE RE Sbjct: 153 KGKHHKGKHAKGKGKKSHPKPED-DSVFFDDERPKHHEFDDEDRE 196 >SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 30.7 bits (66), Expect = 0.55 Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 3/35 (8%) Frame = +1 Query: 658 HHERDRKSEH---NKSSSKDSKRHSGDQKXHKRAR 753 H E+ RK +H ++S DS RHS + H R+R Sbjct: 176 HREKRRKLKHESSDRSDKSDSNRHSRHHRRHSRSR 210 >SPBC1604.09c |||exoribonuclease Rex4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 260 Score = 29.5 bits (63), Expect = 1.3 Identities = 13/43 (30%), Positives = 25/43 (58%) Frame = +1 Query: 652 SSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDESRENDRS 780 SS+ E+ +K + S + K+HS ++K K + ++ EN+ S Sbjct: 3 SSNWEKLKKKLDSAKSGRVEKKHSNEKKYLKSVKGKNNENENS 45 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 27.9 bits (59), Expect = 3.9 Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 1/48 (2%) Frame = +1 Query: 640 KHHKSSHHERDRKSEHNKS-SSKDSKRHSGDQKXHKRARDESRENDRS 780 KH + S HE +S+H SK +++ D+K K SR + S Sbjct: 596 KHKRDSKHEARPESKHESHRESKSAEKDKKDKKDKKEDSKRSRSHSVS 643 >SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Par2|Schizosaccharomyces pombe|chr 1|||Manual Length = 627 Score = 27.9 bits (59), Expect = 3.9 Identities = 11/43 (25%), Positives = 17/43 (39%) Frame = +1 Query: 637 DKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDESR 765 D HH S H + KS K + K + H + K + + Sbjct: 47 DTHHSSKHELKKLKSHFLKDTLKHKRNHHANSNNEKHENSDKK 89 >SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 27.5 bits (58), Expect = 5.1 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +2 Query: 302 IVKKKMKNPVRTGRIWNEKRPRKIKRSVSTTVKISTVNGREGTPD 436 I+K +K+ VR I N P+K KR++ +K G+PD Sbjct: 307 IIKLPLKDNVRPREIGNVNNPKKRKRALELALK----GSESGSPD 347 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 26.6 bits (56), Expect = 8.9 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +1 Query: 634 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKXHKRARDE 759 R ++ S++ R+R SE N +S D+KR + K R + Sbjct: 1484 RVSNNNSTNVSRERSSEANHRTSNDNKRDEVTEGKDKNKRQD 1525 >SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 26.6 bits (56), Expect = 8.9 Identities = 15/53 (28%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Frame = -3 Query: 403 NLHGRRYAPFYLPRPLLVPDPTSPYRILHLLLHYPQ-THPTHLTPRNRNRNRH 248 N H Y L L+ D +H+ Q +HPTH +P+N ++ R+ Sbjct: 80 NKHNEEYFRLLLAANALLSDKRRREEYDRFGIHWNQPSHPTHPSPQNWSQARY 132 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,761,442 Number of Sequences: 5004 Number of extensions: 41823 Number of successful extensions: 216 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 188 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 209 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 846388298 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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