Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_F05_e614_11.seq
(1568 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 101 2e-21
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 96 6e-20
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 96 6e-20
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 94 3e-19
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 93 3e-19
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 86 5e-17
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 86 5e-17
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 83 5e-16
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 80 3e-15
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 78 1e-14
At1g79930.1 68414.m09340 heat shock protein, putative contains P... 42 0.001
At4g16660.1 68417.m02517 heat shock protein 70, putative / HSP70... 37 0.032
At1g11660.1 68414.m01339 heat shock protein, putative strong sim... 32 0.90
At1g15940.1 68414.m01913 expressed protein similar To androgen-i... 31 2.1
At5g16730.1 68418.m01959 expressed protein weak similarity to mi... 31 2.7
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 101 bits (241), Expect = 2e-21
Identities = 41/80 (51%), Positives = 63/80 (78%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEY 192
K +++KN+LE+Y +N ++T++DEKL K+T DKQ I ++TI+W++ NQLA +E+
Sbjct: 538 KKKVEAKNSLENYAYNMRNTIKDEKLAQKLTQEDKQKIEKAIDETIEWIEGNQLAEVDEF 597
Query: 193 EHKQKELEGVCNPIITKLYQ 252
E+K KELEG+CNPII+K+YQ
Sbjct: 598 EYKLKELEGICNPIISKMYQ 617
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 95.9 bits (228), Expect = 6e-20
Identities = 39/80 (48%), Positives = 59/80 (73%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEY 192
K +++KNALE+Y +N ++T++DEK+ +K+ +DK+ I D I+WL+ NQLA +E+
Sbjct: 539 KKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEF 598
Query: 193 EHKQKELEGVCNPIITKLYQ 252
E K KELE +CNPII K+YQ
Sbjct: 599 EDKMKELESICNPIIAKMYQ 618
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 95.9 bits (228), Expect = 6e-20
Identities = 39/80 (48%), Positives = 58/80 (72%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEY 192
K +++KNALE+Y +N ++T+ DEK+ +K+ +DK+ + D + I+WLD NQL +E+
Sbjct: 539 KKKVEAKNALENYAYNMRNTIRDEKIGEKLPAADKKKVEDSIEEAIQWLDGNQLGEADEF 598
Query: 193 EHKQKELEGVCNPIITKLYQ 252
E K KELE VCNPII K+YQ
Sbjct: 599 EDKMKELESVCNPIIAKMYQ 618
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 93.9 bits (223), Expect = 3e-19
Identities = 39/80 (48%), Positives = 58/80 (72%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEY 192
K + +KNALE+Y +N ++T++DEK+ K+ +DK+ I D + I+WLD NQLA +E+
Sbjct: 539 KKKVDAKNALENYAYNMRNTIKDEKIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEF 598
Query: 193 EHKQKELEGVCNPIITKLYQ 252
E K KELE +CNPII ++YQ
Sbjct: 599 EDKMKELESLCNPIIARMYQ 618
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 93.5 bits (222), Expect = 3e-19
Identities = 39/80 (48%), Positives = 57/80 (71%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEY 192
K + +KNALE+Y +N ++T+ DEK+ +K+ DK+ I D I+WL++NQLA +E+
Sbjct: 539 KKKVDAKNALENYAYNMRNTIRDEKIGEKLAGDDKKKIEDSIEAAIEWLEANQLAECDEF 598
Query: 193 EHKQKELEGVCNPIITKLYQ 252
E K KELE +CNPII K+YQ
Sbjct: 599 EDKMKELESICNPIIAKMYQ 618
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 86.2 bits (204), Expect = 5e-17
Identities = 39/84 (46%), Positives = 57/84 (67%), Gaps = 1/84 (1%)
Frame = +1
Query: 4 KNSKDTIQSKNALESYCFNXKSTMED-EKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAX 180
K K+ I ++NALE+Y +N K+ + D +KL DK+ +K+ I + ++WLD NQ +
Sbjct: 561 KKVKEKIDARNALETYVYNMKNQVSDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSE 620
Query: 181 KEEYEHKQKELEGVCNPIITKLYQ 252
KEEY+ K KE+E VCNPIIT +YQ
Sbjct: 621 KEEYDEKLKEVEAVCNPIITAVYQ 644
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 86.2 bits (204), Expect = 5e-17
Identities = 39/84 (46%), Positives = 57/84 (67%), Gaps = 1/84 (1%)
Frame = +1
Query: 4 KNSKDTIQSKNALESYCFNXKSTMED-EKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAX 180
K K+ I ++NALE+Y +N K+ + D +KL DK+ +K+ I + ++WLD NQ +
Sbjct: 561 KKVKEKIDARNALETYVYNMKNQVNDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSE 620
Query: 181 KEEYEHKQKELEGVCNPIITKLYQ 252
KEEY+ K KE+E VCNPIIT +YQ
Sbjct: 621 KEEYDEKLKEVEAVCNPIITAVYQ 644
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 83.0 bits (196), Expect = 5e-16
Identities = 37/80 (46%), Positives = 55/80 (68%), Gaps = 1/80 (1%)
Frame = +1
Query: 16 DTIQSKNALESYCFNXKSTMED-EKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEY 192
+ I ++NALE+Y +N K+ + D +KL DK+ +K+ I + ++WLD NQ + KEEY
Sbjct: 510 EKIDARNALETYVYNMKNQVSDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEY 569
Query: 193 EHKQKELEGVCNPIITKLYQ 252
+ K KE+E VCNPIIT +YQ
Sbjct: 570 DEKLKEVEAVCNPIITAVYQ 589
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 80.2 bits (189), Expect = 3e-15
Identities = 36/80 (45%), Positives = 54/80 (67%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEY 192
K +++KN LE+Y +N +T+ D + +K+ +DK+ D + I+WLD NQLA +E+
Sbjct: 539 KKKVEAKNGLENYAYNVGNTLRD--MGEKLPAADKKKFEDSIEEVIQWLDDNQLAEADEF 596
Query: 193 EHKQKELEGVCNPIITKLYQ 252
EHK KELE V + IITK+YQ
Sbjct: 597 EHKMKELESVWSTIITKMYQ 616
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 78.2 bits (184), Expect = 1e-14
Identities = 36/84 (42%), Positives = 56/84 (66%), Gaps = 1/84 (1%)
Frame = +1
Query: 4 KNSKDTIQSKNALESYCFNXKSTMED-EKLKDKITDSDKQIIMDKCNDTIKWLDSNQLAX 180
K K+ I ++N LE+Y +N KST+ D EKL KI+D DK+ + + ++WL+ N A
Sbjct: 575 KIMKEKIDARNKLETYVYNMKSTVADKEKLAKKISDEDKEKMEGVLKEALEWLEENVNAE 634
Query: 181 KEEYEHKQKELEGVCNPIITKLYQ 252
KE+Y+ K KE+E VC+P+I +Y+
Sbjct: 635 KEDYDEKLKEVELVCDPVIKSVYE 658
>At1g79930.1 68414.m09340 heat shock protein, putative contains Pfam
profile: PF00012 Heat shock hsp70 proteins; similar to
heat-shock proteins GB:CAA94389, GB:AAD55461
[Arabidopsis thaliana]
Length = 831
Score = 41.9 bits (94), Expect = 0.001
Identities = 22/75 (29%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWL-DSNQLAXKEE 189
++T KNA+ESY ++ ++ + D K ++ ITDS+++ + + WL + + K
Sbjct: 617 EETKDRKNAVESYVYDMRNKLSD-KYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGV 675
Query: 190 YEHKQKELEGVCNPI 234
Y K +EL+ V +P+
Sbjct: 676 YVAKLEELKKVGDPV 690
>At4g16660.1 68417.m02517 heat shock protein 70, putative / HSP70,
putative
Length = 867
Score = 37.1 bits (82), Expect = 0.032
Identities = 19/78 (24%), Positives = 39/78 (50%), Gaps = 1/78 (1%)
Frame = +1
Query: 4 KNSKDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWL-DSNQLAX 180
+ + T + KN LESY + K +E + + T +++ ++K ++ WL + A
Sbjct: 654 RERRRTAELKNNLESYIYATKEKLETPEFEKISTQEERKAFVEKLDEVQDWLYMDGEDAN 713
Query: 181 KEEYEHKQKELEGVCNPI 234
E+E + L+ + +PI
Sbjct: 714 ATEFEKRLDSLKAIGSPI 731
>At1g11660.1 68414.m01339 heat shock protein, putative strong
similarity to gb|Z70314 heat-shock protein from
Arabidopsis thaliana and is a member of the PF|00012
Hsp70 protein family
Length = 773
Score = 32.3 bits (70), Expect = 0.90
Identities = 18/75 (24%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +1
Query: 13 KDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQIIMDKCNDTIKWL-DSNQLAXKEE 189
+ T KNALES+ + + M + ++ T+S+++ I +T +WL + +
Sbjct: 586 ESTKDKKNALESFVYEMRDKMLN-TYRNTATESERECIARNLQETEEWLYEDGDDESENA 644
Query: 190 YEHKQKELEGVCNPI 234
Y K +++ + +PI
Sbjct: 645 YIEKLNDVKKLIDPI 659
>At1g15940.1 68414.m01913 expressed protein similar To
androgen-induced prostate proliferative shutoff
associated protein (GI:4559410) [Homo sapiens]
Length = 990
Score = 31.1 bits (67), Expect = 2.1
Identities = 17/39 (43%), Positives = 22/39 (56%)
Frame = +1
Query: 4 KNSKDTIQSKNALESYCFNXKSTMEDEKLKDKITDSDKQ 120
K+ K I SKN S +S+M+ K KD +TDS KQ
Sbjct: 651 KSKKRKIVSKNVEPSSSPEVRSSMQTMKKKDSVTDSIKQ 689
>At5g16730.1 68418.m01959 expressed protein weak similarity to
microtubule binding protein D-CLIP-190 [Drosophila
melanogaster] GI:2773363, SMC2-like condensin
[Arabidopsis thaliana] GI:14279543
Length = 853
Score = 30.7 bits (66), Expect = 2.7
Identities = 22/63 (34%), Positives = 29/63 (46%), Gaps = 2/63 (3%)
Frame = +1
Query: 34 NALESYCFNXKSTMEDEKL--KDKITDSDKQIIMDKCNDTIKWLDSNQLAXKEEYEHKQK 207
N +E N K +E EK KD+ D DK D K +S Q+ KE + K+
Sbjct: 730 NGMEEKEVNGKPEVETEKKEKKDESQDDDKD---DSVEVIFKMWESCQIEKKEAFPDKKS 786
Query: 208 ELE 216
ELE
Sbjct: 787 ELE 789
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,953,028
Number of Sequences: 28952
Number of extensions: 117172
Number of successful extensions: 514
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 491
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 508
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4221703296
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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