Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_F02_e590_12.seq
(1557 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19090.1 68418.m02269 heavy-metal-associated domain-containin... 43 5e-04
At2g18470.1 68415.m02151 protein kinase family protein contains ... 35 0.17
At5g49280.1 68418.m06099 hydroxyproline-rich glycoprotein family... 33 0.51
At5g44500.1 68418.m05452 small nuclear ribonucleoprotein associa... 32 1.2
At3g11820.2 68416.m01448 syntaxin 121 (SYP121) / syntaxin-relate... 31 1.6
At3g11820.1 68416.m01449 syntaxin 121 (SYP121) / syntaxin-relate... 31 1.6
At3g44340.1 68416.m04764 sec23/sec24 transport family protein co... 31 2.1
At2g04170.1 68415.m00402 meprin and TRAF homology domain-contain... 31 2.1
At3g06130.1 68416.m00704 heavy-metal-associated domain-containin... 31 2.7
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 30 3.6
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 30 3.6
At4g25630.1 68417.m03691 fibrillarin 2 (FIB2) identical to fibri... 30 3.6
At1g31810.1 68414.m03904 formin homology 2 domain-containing pro... 30 3.6
At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family... 29 8.3
At3g21215.1 68416.m02681 RNA-binding protein, putative contains ... 29 8.3
At3g15000.1 68416.m01897 expressed protein similar to DAG protei... 29 8.3
>At5g19090.1 68418.m02269 heavy-metal-associated domain-containing
protein contains Pfam heavy-metal-associated domain
PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
isoform contains a non-consensus TG-acceptor splice site
at intron 3
Length = 587
Score = 43.2 bits (97), Expect = 5e-04
Identities = 23/58 (39%), Positives = 24/58 (41%), Gaps = 2/58 (3%)
Frame = -1
Query: 1551 GGXGXGXXGGXRGGVPRXXRP--XGGGPPXPPXXRXXPXGGXRXGPXXPPPXFXGXPG 1384
GG G G G GG+P RP GGG P P GG GP P G PG
Sbjct: 388 GGGGGGGGGPMSGGLPPGFRPMGGGGGGGGGPQSMSMPMGGAMGGPMGSLPQMGGGPG 445
>At2g18470.1 68415.m02151 protein kinase family protein contains Pfam
PF00069: Protein kinase domain
Length = 633
Score = 34.7 bits (76), Expect = 0.17
Identities = 17/44 (38%), Positives = 17/44 (38%)
Frame = -1
Query: 1551 GGXGXGXXGGXRGGVPRXXRPXGGGPPXPPXXRXXPXGGXRXGP 1420
GG G GG GG P G PP PP GG R P
Sbjct: 78 GGGGERGNGGNNGG--NDTPPSRGSPPSPPSRSNGDNGGSRSSP 119
>At5g49280.1 68418.m06099 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 162
Score = 33.1 bits (72), Expect = 0.51
Identities = 22/63 (34%), Positives = 24/63 (38%), Gaps = 2/63 (3%)
Frame = +2
Query: 1370 TVCTLPGXPXXXGGGXXGPXLXPPXGXXRXXGGXGGPPPXGLXXRG--TPPRXPPXXPXP 1543
T C P P GGG PP + GG PPP G +G PP P P
Sbjct: 63 TACPPPPSPPSSGGGSS--YYYPPPS--QSGGGSKYPPPYGGGGQGYYYPPPYSGNYPTP 118
Query: 1544 XPP 1552
PP
Sbjct: 119 PPP 121
>At5g44500.1 68418.m05452 small nuclear ribonucleoprotein associated
protein B, putative / snRNP-B, putative / Sm protein B,
putative similar to SP|P27048 Small nuclear
ribonucleoprotein associated protein B (snRNP-B) (Sm
protein B) (Sm-B) (SmB) {Mus musculus}
Length = 254
Score = 31.9 bits (69), Expect = 1.2
Identities = 20/56 (35%), Positives = 23/56 (41%)
Frame = +2
Query: 1385 PGXPXXXGGGXXGPXLXPPXGXXRXXGGXGGPPPXGLXXRGTPPRXPPXXPXPXPP 1552
PG P GG P + PP G G PPP G+ +G PP P P P
Sbjct: 184 PGPPPPQYGGQQRPMMIPPPGGMM----RGPPPPHGM--QGPPPSRPGMPPPGGAP 233
>At3g11820.2 68416.m01448 syntaxin 121 (SYP121) / syntaxin-related
protein (SYR1) contains Pfam profiles: PF00804 syntaxin
and PF05739: SNARE domain; identical to cDNA
syntaxin-related protein At-SYR1 (At-Syr1) GI:4206788,
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 315
Score = 31.5 bits (68), Expect = 1.6
Identities = 15/33 (45%), Positives = 16/33 (48%)
Frame = -1
Query: 1551 GGXGXGXXGGXRGGVPRXXRPXGGGPPXPPXXR 1453
GG G G GG GG +P G PP PP R
Sbjct: 283 GGGGGGGGGGTTGG----SQPNSGTPPNPPQAR 311
>At3g11820.1 68416.m01449 syntaxin 121 (SYP121) / syntaxin-related
protein (SYR1) contains Pfam profiles: PF00804 syntaxin
and PF05739: SNARE domain; identical to cDNA
syntaxin-related protein At-SYR1 (At-Syr1) GI:4206788,
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 346
Score = 31.5 bits (68), Expect = 1.6
Identities = 15/33 (45%), Positives = 16/33 (48%)
Frame = -1
Query: 1551 GGXGXGXXGGXRGGVPRXXRPXGGGPPXPPXXR 1453
GG G G GG GG +P G PP PP R
Sbjct: 314 GGGGGGGGGGTTGG----SQPNSGTPPNPPQAR 342
>At3g44340.1 68416.m04764 sec23/sec24 transport family protein
contains Pfam domains PF04811: Sec23/Sec24 trunk domain,
PF04815: Sec23/Sec24 helical domain and PF04810:
Sec23/Sec24 zinc finger
Length = 1096
Score = 31.1 bits (67), Expect = 2.1
Identities = 20/53 (37%), Positives = 20/53 (37%), Gaps = 4/53 (7%)
Frame = -1
Query: 1551 GGXGXGXXGGXRGGVPRXXRPXGGG--PPXPPXXRXXPXGGXRXGPXX--PPP 1405
G G GG G P RP G G PP P P G GP PPP
Sbjct: 146 GFPASGPPGGVPSGPPSGARPIGFGSPPPMGPGMSMPPPSGMPGGPLSNGPPP 198
>At2g04170.1 68415.m00402 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to NtN2 [Medicago truncatula] GI:3776084; contains Pfam
profile PF00917: MATH domain
Length = 420
Score = 31.1 bits (67), Expect = 2.1
Identities = 21/55 (38%), Positives = 21/55 (38%)
Frame = -1
Query: 1548 GXGXGXXGGXRGGVPRXXRPXGGGPPXPPXXRXXPXGGXRXGPXXPPPXFXGXPG 1384
G G G G PR RP GGG P P G GP P P G PG
Sbjct: 61 GPGFGPRGPGPWSGPRGPRPGGGGGPGP---------GPWSGPRGPRPGGGGGPG 106
>At3g06130.1 68416.m00704 heavy-metal-associated domain-containing
protein contains Pfam heavy metal associated domain
PF00403
Length = 473
Score = 30.7 bits (66), Expect = 2.7
Identities = 21/57 (36%), Positives = 21/57 (36%), Gaps = 2/57 (3%)
Frame = -1
Query: 1551 GGXGXGXXGGXRGGVPRXXRPXGGGPPXPPXXRXXPXGG--XRXGPXXPPPXFXGXP 1387
G G G G GGV RP GGG P P GG GP P G P
Sbjct: 313 GKKGNGGGGPMAGGVSGGFRPMGGG---GPQNMSMPMGGQMGMGGPMGNMPAVQGLP 366
>At5g54650.2 68418.m06805 formin homology 2 domain-containing protein
/ FH2 domain-containing protein contains formin homology
2 domain, Pfam:PF02181
Length = 900
Score = 30.3 bits (65), Expect = 3.6
Identities = 15/35 (42%), Positives = 15/35 (42%)
Frame = -1
Query: 1509 VPRXXRPXGGGPPXPPXXRXXPXGGXRXGPXXPPP 1405
VP P GPP PP P G GP PPP
Sbjct: 374 VPAPQMPSSAGPPRPPPPAPPPGSG---GPKPPPP 405
>At5g54650.1 68418.m06804 formin homology 2 domain-containing protein
/ FH2 domain-containing protein contains formin homology
2 domain, Pfam:PF02181
Length = 900
Score = 30.3 bits (65), Expect = 3.6
Identities = 15/35 (42%), Positives = 15/35 (42%)
Frame = -1
Query: 1509 VPRXXRPXGGGPPXPPXXRXXPXGGXRXGPXXPPP 1405
VP P GPP PP P G GP PPP
Sbjct: 374 VPAPQMPSSAGPPRPPPPAPPPGSG---GPKPPPP 405
>At4g25630.1 68417.m03691 fibrillarin 2 (FIB2) identical to
fibrillarin 2 GI:9965655 from [Arabidopsis thaliana]
Length = 320
Score = 30.3 bits (65), Expect = 3.6
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = -1
Query: 1551 GGXGXGXXGGXRGGVPRXXRPXGGGPP 1471
GG G G GG RG R R G GPP
Sbjct: 32 GGRGGGGRGGGRGFSDRGGRGRGRGPP 58
>At1g31810.1 68414.m03904 formin homology 2 domain-containing protein
/ FH2 domain-containing protein low similarity to
SP|P48608 Diaphanous protein {Drosophila melanogaster};
contains Pfam profile PF02181: Formin Homology 2(FH2)
Domain
Length = 1201
Score = 30.3 bits (65), Expect = 3.6
Identities = 15/35 (42%), Positives = 15/35 (42%)
Frame = +2
Query: 1424 PXLXPPXGXXRXXGGXGGPPPXGLXXRGTPPRXPP 1528
P PP G R G GPPP G PP PP
Sbjct: 737 PVPPPPPGLGR--GTSSGPPPLGAKGSNAPPPPPP 769
>At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family
protein Common family members: At4g18570, At4g04980,
At5g61090 [Arabidopsis thaliana]; identical to cDNA
CHUP1 for actin binding protein GI:28071264
Length = 1004
Score = 29.1 bits (62), Expect = 8.3
Identities = 17/43 (39%), Positives = 18/43 (41%), Gaps = 2/43 (4%)
Frame = -1
Query: 1527 GGXRGGVP--RXXRPXGGGPPXPPXXRXXPXGGXRXGPXXPPP 1405
GG +P R P GG PP PP P GP PPP
Sbjct: 661 GGKSTNLPSARPPLPGGGPPPPPPPPGGGPPPPPGGGPPPPPP 703
>At3g21215.1 68416.m02681 RNA-binding protein, putative contains RNA
recognition motif, Pfam:PF00076; contains AT-AC splice
sites at intron 8
Length = 339
Score = 29.1 bits (62), Expect = 8.3
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +2
Query: 1472 GGPPPXGLXXRGTPPRXPPXXPXPXPP 1552
G PPP PP PP P PP
Sbjct: 17 GAPPPPAAVSSAAPPHPPPIHHHPPPP 43
>At3g15000.1 68416.m01897 expressed protein similar to DAG protein
(required for chloroplast differentiation and palisade
development) GB:Q38732 [Antirrhinum majus]
Length = 395
Score = 29.1 bits (62), Expect = 8.3
Identities = 15/40 (37%), Positives = 15/40 (37%)
Frame = -1
Query: 1512 GVPRXXRPXGGGPPXPPXXRXXPXGGXRXGPXXPPPXFXG 1393
G P RP GGPP PP G PPP G
Sbjct: 239 GGPPPQRPPMGGPPPPPHIGGSAPPPPHMGGSAPPPPHMG 278
Score = 29.1 bits (62), Expect = 8.3
Identities = 14/37 (37%), Positives = 14/37 (37%)
Frame = -1
Query: 1515 GGVPRXXRPXGGGPPXPPXXRXXPXGGXRXGPXXPPP 1405
GG P P GG PP P P G PPP
Sbjct: 239 GGPPPQRPPMGGPPPPPHIGGSAPPPPHMGGSAPPPP 275
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,718,511
Number of Sequences: 28952
Number of extensions: 460655
Number of successful extensions: 1223
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1146
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4183148928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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