BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_E10_e653_10.seq (1582 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|... 100 6e-22 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 36 0.015 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.14 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 28 4.1 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.4 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 5.4 SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre... 27 7.1 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 7.1 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 9.4 >SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 100 bits (239), Expect = 6e-22 Identities = 40/92 (43%), Positives = 63/92 (68%) Frame = +2 Query: 347 MAAKVMPETEEQNRIRFQVELEFVQCLANPNYIHFLAQRGYLKEQTFVNYLKYLQYWREP 526 M K + ++ RF++ELEFVQ L+NP Y++FLAQ Y +++ F+ YL+Y++YWREP Sbjct: 1 METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP 60 Query: 527 EYARYLKYPMCLHFLELMQHEAFRRECXSAQV 622 EY +++ YP CLH L L+++ FR + A + Sbjct: 61 EYVKFIIYPTCLHMLTLLKNPQFRNDISRADL 92 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 35.9 bits (79), Expect = 0.015 Identities = 29/124 (23%), Positives = 34/124 (27%), Gaps = 6/124 (4%) Frame = +3 Query: 1215 PPQGXTXXXGPXXRPXFXPGPXPSPPGXPXXXDPPPXXHNAPRRXXPF----SPXXXXXG 1382 PP P +P P P+P G P P P P Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190 Query: 1383 LGAPXSXXAXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXPXAPPPPXPXPXPPXP--S 1556 + P V P + PP G + P A PP P P P S Sbjct: 1191 VPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVS 1250 Query: 1557 TPLS 1568 TP S Sbjct: 1251 TPRS 1254 Score = 29.9 bits (64), Expect = 1.0 Identities = 29/116 (25%), Positives = 33/116 (28%), Gaps = 3/116 (2%) Frame = +3 Query: 1215 PPQGXTXXXGPXXRPXFXPGPXPSPPGXPXXXDPPPXXHNAPRRXXPFSPXXXXXG-LGA 1391 PP P P P S G P P P AP P P + A Sbjct: 1032 PPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVP----APSSEIPSIPAPSGAPPVPA 1087 Query: 1392 PXSXXAXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXPX--APPPPXPXPXPPXP 1553 P V P + P +G + P APP P P PP P Sbjct: 1088 PSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVP 1143 Score = 28.3 bits (60), Expect = 3.1 Identities = 32/121 (26%), Positives = 36/121 (29%), Gaps = 8/121 (6%) Frame = +3 Query: 1215 PPQGXTXXXGPXXRPXFXPGPXPSPPGXPXXXDP----PPXXHNAPRRXXPF-SPXXXXX 1379 PP P +P P P P G P P PP AP P P Sbjct: 1112 PPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPV--PAPSGAPPVPKPSVAAP 1169 Query: 1380 GLGAPXSXX-AXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXP-XAPPPPXPXP-XPPX 1550 + AP S P + PP G + P APP P P PP Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPV 1229 Query: 1551 P 1553 P Sbjct: 1230 P 1230 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 32.7 bits (71), Expect = 0.14 Identities = 31/136 (22%), Positives = 37/136 (27%), Gaps = 7/136 (5%) Frame = +3 Query: 1170 PXGXGXXXXXXLXXGPPQGXTXXXGPXXRPXFXPGPXPSPP-GXPXXXDPPPXXHNAPRR 1346 P G G PP P P P PP P PP ++ Sbjct: 326 PIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAV 385 Query: 1347 XXPFSPXXXXXGLGAPX-----SXXAXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXPX 1511 P +P G AP + V P PP + P Sbjct: 386 SNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPA 445 Query: 1512 APP-PPXPXPXPPXPS 1556 APP PP PP P+ Sbjct: 446 APPLPPSAPIAPPLPA 461 Score = 28.7 bits (61), Expect = 2.3 Identities = 26/98 (26%), Positives = 29/98 (29%) Frame = +3 Query: 1269 PGPXPSPPGXPXXXDPPPXXHNAPRRXXPFSPXXXXXGLGAPXSXXAXXXXGVXCPXLXP 1448 P P P+ PG PP NA R P P AP S + P P Sbjct: 389 PAPPPAIPGRSAPALPP--LGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSL--PMGAP 444 Query: 1449 PXXXXXSAXHXTGXRXLXXPXAPPPPXPXPXPPXPSTP 1562 + P APP P P PP P Sbjct: 445 AAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482 Score = 28.3 bits (60), Expect = 3.1 Identities = 12/27 (44%), Positives = 13/27 (48%), Gaps = 1/27 (3%) Frame = +1 Query: 1480 PLAXG-PSXPRXPPXPPXPXPXPXXRP 1557 PL G P+ P PP P P P P P Sbjct: 458 PLPAGMPAAPPLPPAAPAPPPAPAPAP 484 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 27.9 bits (59), Expect = 4.1 Identities = 17/43 (39%), Positives = 18/43 (41%), Gaps = 1/43 (2%) Frame = -1 Query: 1339 GALXXFGGG-SXXXGXPGGXGLGPGXKXGRXXGPXXXVXPWGG 1214 G FGGG G PGG G GPG G G +GG Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGG 263 Score = 27.5 bits (58), Expect = 5.4 Identities = 14/29 (48%), Positives = 14/29 (48%), Gaps = 1/29 (3%) Frame = -1 Query: 1339 GALXXFGGG-SXXXGXPGGXGLGPGXKXG 1256 G FGGG G PGG G GPG G Sbjct: 242 GGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 27.5 bits (58), Expect = 5.4 Identities = 18/58 (31%), Positives = 22/58 (37%), Gaps = 2/58 (3%) Frame = +1 Query: 1378 GGWAPLXLXARPXXXXXXAXXSPPXXXXXXQPXTPLAXGP--SXPRXPPXPPXPXPXP 1545 GG AP + P A +PP P P++ P S P P PP P P Sbjct: 1679 GGMAPAHPVSTPPVRPQSA--APPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.5 bits (58), Expect = 5.4 Identities = 9/17 (52%), Positives = 10/17 (58%) Frame = +3 Query: 1515 PPPPXPXPXPPXPSTPL 1565 PPPP P P P+ PL Sbjct: 7 PPPPAPAPAAAAPAPPL 23 >SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre1|Schizosaccharomyces pombe|chr 2|||Manual Length = 900 Score = 27.1 bits (57), Expect = 7.1 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = +3 Query: 552 QCVCIFWNLCNMKHSEESV 608 +CV FWN +HS+ SV Sbjct: 616 KCVAFFWNAAKKQHSKSSV 634 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 27.1 bits (57), Expect = 7.1 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +3 Query: 1257 PXFXPGPXPSPPGXPXXXDPPP 1322 P P P P PP P PPP Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPP 763 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 26.6 bits (56), Expect = 9.4 Identities = 13/45 (28%), Positives = 18/45 (40%) Frame = +1 Query: 1405 ARPXXXXXXAXXSPPXXXXXXQPXTPLAXGPSXPRXPPXPPXPXP 1539 ++P + S P +P P+A S P P PP P P Sbjct: 1060 SKPAPSTVTSAASSPSNIS--KPSAPVANNVSKPSAVPPPPPPPP 1102 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,387,013 Number of Sequences: 5004 Number of extensions: 74564 Number of successful extensions: 269 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 240 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 891978300 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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