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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030731E7_E10_e653_10.seq
         (1582 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|...   100   6e-22
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    36   0.015
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    33   0.14 
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    28   4.1  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    27   5.4  
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    27   5.4  
SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre...    27   7.1  
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    27   7.1  
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2...    27   9.4  

>SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit
           Med31|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score =  100 bits (239), Expect = 6e-22
 Identities = 40/92 (43%), Positives = 63/92 (68%)
 Frame = +2

Query: 347 MAAKVMPETEEQNRIRFQVELEFVQCLANPNYIHFLAQRGYLKEQTFVNYLKYLQYWREP 526
           M  K +      ++ RF++ELEFVQ L+NP Y++FLAQ  Y +++ F+ YL+Y++YWREP
Sbjct: 1   METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP 60

Query: 527 EYARYLKYPMCLHFLELMQHEAFRRECXSAQV 622
           EY +++ YP CLH L L+++  FR +   A +
Sbjct: 61  EYVKFIIYPTCLHMLTLLKNPQFRNDISRADL 92


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 35.9 bits (79), Expect = 0.015
 Identities = 29/124 (23%), Positives = 34/124 (27%), Gaps = 6/124 (4%)
 Frame = +3

Query: 1215 PPQGXTXXXGPXXRPXFXPGPXPSPPGXPXXXDPPPXXHNAPRRXXPF----SPXXXXXG 1382
            PP        P  +P     P P+P G P    P       P           P      
Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190

Query: 1383 LGAPXSXXAXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXPXAPPPPXPXPXPPXP--S 1556
            +  P          V  P + PP           G   +  P A  PP P P    P  S
Sbjct: 1191 VPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVS 1250

Query: 1557 TPLS 1568
            TP S
Sbjct: 1251 TPRS 1254



 Score = 29.9 bits (64), Expect = 1.0
 Identities = 29/116 (25%), Positives = 33/116 (28%), Gaps = 3/116 (2%)
 Frame = +3

Query: 1215 PPQGXTXXXGPXXRPXFXPGPXPSPPGXPXXXDPPPXXHNAPRRXXPFSPXXXXXG-LGA 1391
            PP        P   P   P    S  G P    P P    AP    P  P       + A
Sbjct: 1032 PPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVP----APSSEIPSIPAPSGAPPVPA 1087

Query: 1392 PXSXXAXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXPX--APPPPXPXPXPPXP 1553
            P          V  P +  P          +G   +  P   APP P P   PP P
Sbjct: 1088 PSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVP 1143



 Score = 28.3 bits (60), Expect = 3.1
 Identities = 32/121 (26%), Positives = 36/121 (29%), Gaps = 8/121 (6%)
 Frame = +3

Query: 1215 PPQGXTXXXGPXXRPXFXPGPXPSPPGXPXXXDP----PPXXHNAPRRXXPF-SPXXXXX 1379
            PP        P  +P     P P P G P    P    PP    AP    P   P     
Sbjct: 1112 PPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPV--PAPSGAPPVPKPSVAAP 1169

Query: 1380 GLGAPXSXX-AXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXP-XAPPPPXPXP-XPPX 1550
             + AP S            P + PP           G   +  P  APP P P    PP 
Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPV 1229

Query: 1551 P 1553
            P
Sbjct: 1230 P 1230


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 574

 Score = 32.7 bits (71), Expect = 0.14
 Identities = 31/136 (22%), Positives = 37/136 (27%), Gaps = 7/136 (5%)
 Frame = +3

Query: 1170 PXGXGXXXXXXLXXGPPQGXTXXXGPXXRPXFXPGPXPSPP-GXPXXXDPPPXXHNAPRR 1346
            P G G          PP            P     P P PP   P     PP   ++   
Sbjct: 326  PIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAV 385

Query: 1347 XXPFSPXXXXXGLGAPX-----SXXAXXXXGVXCPXLXPPXXXXXSAXHXTGXRXLXXPX 1511
              P +P     G  AP      +        V  P   PP               +  P 
Sbjct: 386  SNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPA 445

Query: 1512 APP-PPXPXPXPPXPS 1556
            APP PP     PP P+
Sbjct: 446  APPLPPSAPIAPPLPA 461



 Score = 28.7 bits (61), Expect = 2.3
 Identities = 26/98 (26%), Positives = 29/98 (29%)
 Frame = +3

Query: 1269 PGPXPSPPGXPXXXDPPPXXHNAPRRXXPFSPXXXXXGLGAPXSXXAXXXXGVXCPXLXP 1448
            P P P+ PG      PP    NA R   P  P        AP S        +  P   P
Sbjct: 389  PAPPPAIPGRSAPALPP--LGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSL--PMGAP 444

Query: 1449 PXXXXXSAXHXTGXRXLXXPXAPPPPXPXPXPPXPSTP 1562
                   +           P APP P   P PP    P
Sbjct: 445  AAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482



 Score = 28.3 bits (60), Expect = 3.1
 Identities = 12/27 (44%), Positives = 13/27 (48%), Gaps = 1/27 (3%)
 Frame = +1

Query: 1480 PLAXG-PSXPRXPPXPPXPXPXPXXRP 1557
            PL  G P+ P  PP  P P P P   P
Sbjct: 458  PLPAGMPAAPPLPPAAPAPPPAPAPAP 484


>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 273

 Score = 27.9 bits (59), Expect = 4.1
 Identities = 17/43 (39%), Positives = 18/43 (41%), Gaps = 1/43 (2%)
 Frame = -1

Query: 1339 GALXXFGGG-SXXXGXPGGXGLGPGXKXGRXXGPXXXVXPWGG 1214
            G    FGGG     G PGG G GPG   G   G       +GG
Sbjct: 221  GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGG 263



 Score = 27.5 bits (58), Expect = 5.4
 Identities = 14/29 (48%), Positives = 14/29 (48%), Gaps = 1/29 (3%)
 Frame = -1

Query: 1339 GALXXFGGG-SXXXGXPGGXGLGPGXKXG 1256
            G    FGGG     G PGG G GPG   G
Sbjct: 242  GGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 18/58 (31%), Positives = 22/58 (37%), Gaps = 2/58 (3%)
 Frame = +1

Query: 1378 GGWAPLXLXARPXXXXXXAXXSPPXXXXXXQPXTPLAXGP--SXPRXPPXPPXPXPXP 1545
            GG AP    + P      A  +PP       P  P++  P  S P  P  PP   P P
Sbjct: 1679 GGMAPAHPVSTPPVRPQSA--APPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734


>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 309

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 9/17 (52%), Positives = 10/17 (58%)
 Frame = +3

Query: 1515 PPPPXPXPXPPXPSTPL 1565
            PPPP P P    P+ PL
Sbjct: 7    PPPPAPAPAAAAPAPPL 23


>SPBC19C2.09 |sre1||sterol regulatory element binding protein
           Sre1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 900

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 9/19 (47%), Positives = 12/19 (63%)
 Frame = +3

Query: 552 QCVCIFWNLCNMKHSEESV 608
           +CV  FWN    +HS+ SV
Sbjct: 616 KCVAFFWNAAKKQHSKSSV 634


>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
            3|||Manual
          Length = 1461

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 10/22 (45%), Positives = 10/22 (45%)
 Frame = +3

Query: 1257 PXFXPGPXPSPPGXPXXXDPPP 1322
            P   P P P PP  P    PPP
Sbjct: 742  PTPAPAPIPVPPPAPIMGGPPP 763


>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1217

 Score = 26.6 bits (56), Expect = 9.4
 Identities = 13/45 (28%), Positives = 18/45 (40%)
 Frame = +1

Query: 1405 ARPXXXXXXAXXSPPXXXXXXQPXTPLAXGPSXPRXPPXPPXPXP 1539
            ++P      +  S P      +P  P+A   S P   P PP P P
Sbjct: 1060 SKPAPSTVTSAASSPSNIS--KPSAPVANNVSKPSAVPPPPPPPP 1102


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,387,013
Number of Sequences: 5004
Number of extensions: 74564
Number of successful extensions: 269
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 240
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 891978300
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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