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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030731E7_E09_e645_09.seq
         (1518 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.)              61   3e-09
SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.)               61   3e-09
SB_56| Best HMM Match : Actin (HMM E-Value=0)                          61   3e-09
SB_56628| Best HMM Match : Actin (HMM E-Value=0)                       61   3e-09
SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.)               61   3e-09
SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.)              53   7e-07
SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07)                  31   3.2  

>SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 376

 Score = 60.9 bits (141), Expect = 3e-09
 Identities = 43/117 (36%), Positives = 49/117 (41%)
 Frame = +3

Query: 198 GPECAKXGXXETTLPSPYFPXYRGATXSPAAXXXXWXTKDXYVG**GTAAGRVCXHSXTX 377
           G    K G      P   FP   G              KD YVG    +   +       
Sbjct: 14  GSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGM-GQKDSYVGDEAQSKRGILTLKYPI 72

Query: 378 SNTAXXPXWDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
            +      WDD E I H+TF NEL VA EEHP LLTEA LN KA  E   T ++FET
Sbjct: 73  EH-GIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFET 127



 Score = 54.4 bits (125), Expect = 2e-07
 Identities = 36/78 (46%), Positives = 40/78 (51%)
 Frame = +2

Query: 164 DEEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHS 343
           D+E A  +V  GS MCK G  GDDA  AVF S+  G      VM  MG           S
Sbjct: 3   DDEIAALVVDNGSGMCKAGFAGDDAPRAVFPSIV-GRPRHQGVMVGMGQKDSYVGDEAQS 61

Query: 344 XRTGMLTLXYPIEHGXXT 397
            R G+LTL YPIEHG  T
Sbjct: 62  KR-GILTLKYPIEHGIVT 78


>SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 375

 Score = 60.9 bits (141), Expect = 3e-09
 Identities = 43/117 (36%), Positives = 49/117 (41%)
 Frame = +3

Query: 198 GPECAKXGXXETTLPSPYFPXYRGATXSPAAXXXXWXTKDXYVG**GTAAGRVCXHSXTX 377
           G    K G      P   FP   G              KD YVG    +   +       
Sbjct: 13  GSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGM-GQKDSYVGDEAQSKRGILTLKYPI 71

Query: 378 SNTAXXPXWDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
            +      WDD E I H+TF NEL VA EEHP LLTEA LN KA  E   T ++FET
Sbjct: 72  EH-GIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFET 126



 Score = 51.6 bits (118), Expect = 2e-06
 Identities = 34/78 (43%), Positives = 40/78 (51%)
 Frame = +2

Query: 164 DEEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHS 343
           +++ A  +V  GS MCK G  GDDA  AVF S+  G      VM  MG           S
Sbjct: 2   EDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIV-GRPRHQGVMVGMGQKDSYVGDEAQS 60

Query: 344 XRTGMLTLXYPIEHGXXT 397
            R G+LTL YPIEHG  T
Sbjct: 61  KR-GILTLKYPIEHGIVT 77


>SB_56| Best HMM Match : Actin (HMM E-Value=0)
          Length = 375

 Score = 60.9 bits (141), Expect = 3e-09
 Identities = 43/117 (36%), Positives = 49/117 (41%)
 Frame = +3

Query: 198 GPECAKXGXXETTLPSPYFPXYRGATXSPAAXXXXWXTKDXYVG**GTAAGRVCXHSXTX 377
           G    K G      P   FP   G              KD YVG    +   +       
Sbjct: 13  GSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGM-GQKDSYVGDEAQSKRGILTLKYPI 71

Query: 378 SNTAXXPXWDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
            +      WDD E I H+TF NEL VA EEHP LLTEA LN KA  E   T ++FET
Sbjct: 72  EH-GIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFET 126



 Score = 52.8 bits (121), Expect = 7e-07
 Identities = 35/78 (44%), Positives = 40/78 (51%)
 Frame = +2

Query: 164 DEEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHS 343
           ++E A  +V  GS MCK G  GDDA  AVF S+  G      VM  MG           S
Sbjct: 2   EDEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIV-GRPRHQGVMVGMGQKDSYVGDEAQS 60

Query: 344 XRTGMLTLXYPIEHGXXT 397
            R G+LTL YPIEHG  T
Sbjct: 61  KR-GILTLKYPIEHGIVT 77


>SB_56628| Best HMM Match : Actin (HMM E-Value=0)
          Length = 376

 Score = 60.9 bits (141), Expect = 3e-09
 Identities = 43/117 (36%), Positives = 49/117 (41%)
 Frame = +3

Query: 198 GPECAKXGXXETTLPSPYFPXYRGATXSPAAXXXXWXTKDXYVG**GTAAGRVCXHSXTX 377
           G    K G      P   FP   G              KD YVG    +   +       
Sbjct: 14  GSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGM-GQKDSYVGDEAQSKRGILTLKYPI 72

Query: 378 SNTAXXPXWDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
            +      WDD E I H+TF NEL VA EEHP LLTEA LN KA  E   T ++FET
Sbjct: 73  EH-GIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFET 127



 Score = 53.2 bits (122), Expect = 5e-07
 Identities = 35/78 (44%), Positives = 40/78 (51%)
 Frame = +2

Query: 164 DEEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHS 343
           D++ A  +V  GS MCK G  GDDA  AVF S+  G      VM  MG           S
Sbjct: 3   DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIV-GRPRHQGVMVGMGQKDSYVGDEAQS 61

Query: 344 XRTGMLTLXYPIEHGXXT 397
            R G+LTL YPIEHG  T
Sbjct: 62  KR-GILTLKYPIEHGIVT 78


>SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 349

 Score = 60.9 bits (141), Expect = 3e-09
 Identities = 43/117 (36%), Positives = 49/117 (41%)
 Frame = +3

Query: 198 GPECAKXGXXETTLPSPYFPXYRGATXSPAAXXXXWXTKDXYVG**GTAAGRVCXHSXTX 377
           G    K G      P   FP   G              KD YVG    +   +       
Sbjct: 14  GSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGM-GQKDSYVGDEAQSKRGILTLKYPI 72

Query: 378 SNTAXXPXWDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
            +      WDD E I H+TF NEL VA EEHP LLTEA LN KA  E   T ++FET
Sbjct: 73  EH-GIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFET 127



 Score = 54.4 bits (125), Expect = 2e-07
 Identities = 35/79 (44%), Positives = 41/79 (51%)
 Frame = +2

Query: 161 ADEEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRH 340
           AD++ A  ++  GS MCK G  GDDA  AVF S+  G      VM  MG           
Sbjct: 2   ADDDIAALVIDNGSGMCKAGFAGDDAPRAVFPSIV-GRPRHQGVMVGMGQKDSYVGDEAQ 60

Query: 341 SXRTGMLTLXYPIEHGXXT 397
           S R G+LTL YPIEHG  T
Sbjct: 61  SKR-GILTLKYPIEHGIVT 78


>SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 338

 Score = 52.8 bits (121), Expect = 7e-07
 Identities = 34/78 (43%), Positives = 40/78 (51%)
 Frame = +2

Query: 164 DEEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHS 343
           D++ A  ++  GS MCK G  GDDA  AVF S+  G      VM  MG           S
Sbjct: 2   DDDVAALVIDNGSGMCKAGFAGDDAPRAVFPSIV-GRPRHQGVMVGMGQKDSYVGDEAQS 60

Query: 344 XRTGMLTLXYPIEHGXXT 397
            R G+LTL YPIEHG  T
Sbjct: 61  KR-GILTLKYPIEHGIVT 77


>SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07)
          Length = 240

 Score = 30.7 bits (66), Expect = 3.2
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = +3

Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFE 545
           WD  E I  Y +   +   +  HP L++EAA N +   E   T L+FE
Sbjct: 12  WDLFEKILDYIYSKNIKSDSALHPLLMSEAAWNTRIKREKL-TELMFE 58


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,388,270
Number of Sequences: 59808
Number of extensions: 168730
Number of successful extensions: 144
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 16,821,457
effective HSP length: 85
effective length of database: 11,737,777
effective search space used: 4929866340
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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