Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_E09_e645_09.seq
(1518 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496... 62 1e-09
At5g59370.1 68418.m07440 actin 4 (ACT4) identical to SP|P53494 A... 61 2e-09
At3g46520.1 68416.m05050 actin 12 (ACT12) identical to SP|P53497... 61 2e-09
At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to S... 61 2e-09
At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493 A... 60 3e-09
At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671 A... 60 3e-09
At3g18780.2 68416.m02386 actin 2 (ACT2) identical to SP|Q96292 A... 60 4e-09
At3g18780.1 68416.m02385 actin 2 (ACT2) identical to SP|Q96292 A... 60 4e-09
At1g49240.1 68414.m05520 actin 8 (ACT8) identical to SP|Q96293 A... 60 4e-09
At2g42090.1 68415.m05204 actin, putative similar to SP|P53496 Ac... 56 5e-08
At2g42170.1 68415.m05219 actin, putative similar to actin 2 [Ara... 56 6e-08
At2g42100.1 68415.m05205 actin, putative very strong similarity ... 54 2e-07
At1g73910.1 68414.m08559 actin-related protein 5 (ARP5) identica... 31 1.5
At1g18450.1 68414.m02302 actin-related protein 4 (ARP4) neary id... 31 1.5
At3g27000.1 68416.m03378 actin-related protein 2 (ARP2) nearly i... 31 2.0
>At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496
Actin 11 {Arabidopsis thaliana}
Length = 377
Score = 61.7 bits (143), Expect = 1e-09
Identities = 45/122 (36%), Positives = 52/122 (42%)
Frame = +3
Query: 198 GPECAKXGXXETTLPSPYFPXYRGATXSPAAXXXXWXTKDXYVG**GTAAGRVCXHSXTX 377
G K G P FP G KD YVG + +
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGM-GQKDAYVGDEAQSKRGILTLKYPI 73
Query: 378 SNTAXXPXWDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFETLQH 557
+ WDD E I H+TF NEL VA EEHP LLTEA LN KA E T ++FET +
Sbjct: 74 EH-GIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFETF-N 130
Query: 558 TP 563
TP
Sbjct: 131 TP 132
>At5g59370.1 68418.m07440 actin 4 (ACT4) identical to SP|P53494
Actin 4 {Arabidopsis thaliana}
Length = 377
Score = 61.3 bits (142), Expect = 2e-09
Identities = 33/54 (61%), Positives = 37/54 (68%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFETLQHTP 563
WDD E I H+TF NEL VA EEHP LLTEA LN KA E T ++FET +TP
Sbjct: 81 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFETF-NTP 132
Score = 41.9 bits (94), Expect = 0.001
Identities = 29/64 (45%), Positives = 33/64 (51%)
Frame = +2
Query: 197 GSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHSXRTGMLTLXYP 376
G+ M K G GDDA AVF S+ G + VM MG S R G+LTL YP
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSIV-GRPRHTGVMVGMGQKDAYVGDEAQSKR-GILTLKYP 72
Query: 377 IEHG 388
IEHG
Sbjct: 73 IEHG 76
>At3g46520.1 68416.m05050 actin 12 (ACT12) identical to SP|P53497
Actin 12 {Arabidopsis thaliana}
Length = 377
Score = 61.3 bits (142), Expect = 2e-09
Identities = 33/54 (61%), Positives = 37/54 (68%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFETLQHTP 563
WDD E I H+TF NEL VA EEHP LLTEA LN KA E T ++FET +TP
Sbjct: 81 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFETF-NTP 132
Score = 41.9 bits (94), Expect = 0.001
Identities = 29/64 (45%), Positives = 33/64 (51%)
Frame = +2
Query: 197 GSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHSXRTGMLTLXYP 376
G+ M K G GDDA AVF S+ G + VM MG S R G+LTL YP
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSIV-GRPRHTGVMVGMGQKDAYVGDEAQSKR-GILTLKYP 72
Query: 377 IEHG 388
IEHG
Sbjct: 73 IEHG 76
>At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to
SP|P53492 Actin 7 (Actin-2) {Arabidopsis thaliana}
Length = 377
Score = 60.9 bits (141), Expect = 2e-09
Identities = 43/117 (36%), Positives = 49/117 (41%)
Frame = +3
Query: 198 GPECAKXGXXETTLPSPYFPXYRGATXSPAAXXXXWXTKDXYVG**GTAAGRVCXHSXTX 377
G K G P FP G KD YVG + +
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGM-GQKDAYVGDEAQSKRGILTLKYPI 73
Query: 378 SNTAXXPXWDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
+ WDD E I H+TF NEL VA EEHP LLTEA LN KA E T ++FET
Sbjct: 74 EH-GIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM-TQIMFET 128
>At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493
Actin 3 {Arabidopsis thaliana}; supported by full-length
cDNA: Ceres: 19581.
Length = 377
Score = 60.5 bits (140), Expect = 3e-09
Identities = 31/49 (63%), Positives = 34/49 (69%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I H+TF NEL VA EEHP LLTEA LN KA E T ++FET
Sbjct: 81 WDDMEKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKM-TQIMFET 128
Score = 41.9 bits (94), Expect = 0.001
Identities = 29/64 (45%), Positives = 33/64 (51%)
Frame = +2
Query: 197 GSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHSXRTGMLTLXYP 376
G+ M K G GDDA AVF S+ G + VM MG S R G+LTL YP
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSIV-GRPRHTGVMVGMGQKDAYVGDEAQSKR-GILTLKYP 72
Query: 377 IEHG 388
IEHG
Sbjct: 73 IEHG 76
>At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671
Actin 1 (Actin 3) {Arabidopsis thaliana}
Length = 377
Score = 60.5 bits (140), Expect = 3e-09
Identities = 31/49 (63%), Positives = 34/49 (69%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I H+TF NEL VA EEHP LLTEA LN KA E T ++FET
Sbjct: 81 WDDMEKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKM-TQIMFET 128
Score = 41.9 bits (94), Expect = 0.001
Identities = 29/64 (45%), Positives = 33/64 (51%)
Frame = +2
Query: 197 GSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHSXRTGMLTLXYP 376
G+ M K G GDDA AVF S+ G + VM MG S R G+LTL YP
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSIV-GRPRHTGVMVGMGQKDAYVGDEAQSKR-GILTLKYP 72
Query: 377 IEHG 388
IEHG
Sbjct: 73 IEHG 76
>At3g18780.2 68416.m02386 actin 2 (ACT2) identical to SP|Q96292
Actin 2 {Arabidopsis thaliana}; nearly identical to
SP|Q96293 Actin 8 [Arabidopsis thaliana] GI:1669387 and
to At1g49240
Length = 377
Score = 60.1 bits (139), Expect = 4e-09
Identities = 30/49 (61%), Positives = 34/49 (69%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I H+TF NEL +A EEHP LLTEA LN KA E T ++FET
Sbjct: 81 WDDMEKIWHHTFYNELRIAPEEHPVLLTEAPLNPKANREKM-TQIMFET 128
Score = 38.3 bits (85), Expect = 0.013
Identities = 28/64 (43%), Positives = 31/64 (48%)
Frame = +2
Query: 197 GSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHSXRTGMLTLXYP 376
G+ M K G GDDA AVF S+ G VM M S R G+LTL YP
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSVV-GRPRHHGVMVGMNQKDAYVGDEAQSKR-GILTLKYP 72
Query: 377 IEHG 388
IEHG
Sbjct: 73 IEHG 76
>At3g18780.1 68416.m02385 actin 2 (ACT2) identical to SP|Q96292
Actin 2 {Arabidopsis thaliana}; nearly identical to
SP|Q96293 Actin 8 [Arabidopsis thaliana] GI:1669387 and
to At1g49240
Length = 371
Score = 60.1 bits (139), Expect = 4e-09
Identities = 30/49 (61%), Positives = 34/49 (69%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I H+TF NEL +A EEHP LLTEA LN KA E T ++FET
Sbjct: 81 WDDMEKIWHHTFYNELRIAPEEHPVLLTEAPLNPKANREKM-TQIMFET 128
Score = 38.3 bits (85), Expect = 0.013
Identities = 28/64 (43%), Positives = 31/64 (48%)
Frame = +2
Query: 197 GSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHSXRTGMLTLXYP 376
G+ M K G GDDA AVF S+ G VM M S R G+LTL YP
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSVV-GRPRHHGVMVGMNQKDAYVGDEAQSKR-GILTLKYP 72
Query: 377 IEHG 388
IEHG
Sbjct: 73 IEHG 76
>At1g49240.1 68414.m05520 actin 8 (ACT8) identical to SP|Q96293
Actin 8 {Arabidopsis thaliana}; nearly identical to
SP|Q96292 Actin 2 [Arabidopsis thaliana] GI:1669387, and
to At3g18780
Length = 377
Score = 60.1 bits (139), Expect = 4e-09
Identities = 30/49 (61%), Positives = 34/49 (69%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I H+TF NEL +A EEHP LLTEA LN KA E T ++FET
Sbjct: 81 WDDMEKIWHHTFYNELRIAPEEHPVLLTEAPLNPKANREKM-TQIMFET 128
Score = 38.3 bits (85), Expect = 0.013
Identities = 28/64 (43%), Positives = 31/64 (48%)
Frame = +2
Query: 197 GSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRRLMRHSXRTGMLTLXYP 376
G+ M K G GDDA AVF S+ G VM M S R G+LTL YP
Sbjct: 15 GTGMVKAGFAGDDAPRAVFPSVV-GRPRHHGVMVGMNQKDAYVGDEAQSKR-GILTLKYP 72
Query: 377 IEHG 388
IEHG
Sbjct: 73 IEHG 76
>At2g42090.1 68415.m05204 actin, putative similar to SP|P53496 Actin
11 {Arabidopsis thaliana}; contains Pfam profile
PF00022: Actin
Length = 366
Score = 56.4 bits (130), Expect = 5e-08
Identities = 29/49 (59%), Positives = 32/49 (65%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I HYTF NEL V EEHP LLTEA N KA E T ++FE+
Sbjct: 70 WDDMEKIWHYTFYNELRVDPEEHPVLLTEAPYNPKANREKM-TQIMFES 117
>At2g42170.1 68415.m05219 actin, putative similar to actin 2
[Arabidopsis thaliana] gi|9293903|dbj|BAB01806
Length = 329
Score = 56.0 bits (129), Expect = 6e-08
Identities = 29/49 (59%), Positives = 34/49 (69%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I ++TF +EL VA EEHP LLTEA LN KA E T ++FET
Sbjct: 36 WDDMEKIWYHTFYSELRVAPEEHPVLLTEAPLNPKADREKM-TQIMFET 83
>At2g42100.1 68415.m05205 actin, putative very strong similarity to
SP|P53496 Actin 11 {Arabidopsis thaliana}, SP|P53493
Actin 3 {Arabidopsis thaliana}; contains Pfam profile
PF00022: Actin
Length = 378
Score = 54.0 bits (124), Expect = 2e-07
Identities = 27/49 (55%), Positives = 32/49 (65%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFET 548
WDD E I H+TF NEL + EEHP LLTEA LN K E T ++FE+
Sbjct: 82 WDDMEKIWHHTFYNELRLEPEEHPILLTEAPLNPKVNREKM-TQIMFES 129
Score = 38.7 bits (86), Expect = 0.010
Identities = 31/80 (38%), Positives = 36/80 (45%)
Frame = +2
Query: 149 MSQCADEEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSLSWGDLXTSXVMXXMGH*GLLRR 328
MS DE A + G+ M K G GDDA AVF S+ G VM M
Sbjct: 1 MSDLGDESVAI-VCDNGTGMVKAGFAGDDAPRAVFPSVV-GRPRHRGVMVGMDEKDTFVG 58
Query: 329 LMRHSXRTGMLTLXYPIEHG 388
R G+L+L YPIEHG
Sbjct: 59 -DEAQARRGILSLKYPIEHG 77
>At1g73910.1 68414.m08559 actin-related protein 5 (ARP5) identical
to actin-related protein 5 (ARP5) GI:21489922 from
[Arabidopsis thaliana]
Length = 145
Score = 31.5 bits (68), Expect = 1.5
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +2
Query: 167 EEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSL 262
+E + +V GS CK G G+DA AVF S+
Sbjct: 5 DEVSAIVVDLGSHTCKAGYAGEDAPKAVFPSV 36
Score = 30.7 bits (66), Expect = 2.6
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXK 506
WD + + + F N L + EHP LL E LN +
Sbjct: 97 WDMVDNVWDHAFRNCLMIDPTEHPMLLAEPPLNSQ 131
>At1g18450.1 68414.m02302 actin-related protein 4 (ARP4) neary
identical to actin-related protein 4 (ARP4) [Arabidopsis
thaliana] GI:21427463; contains Pfam profile PF00022:
Actin; supporting cDNA gi|21427462|gb|AF507912.1|
Length = 441
Score = 31.5 bits (68), Expect = 1.5
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +2
Query: 167 EEXAXXIVYYGSXMCKXGXXGDDAXLAVFSSL 262
+E + +V GS CK G G+DA AVF S+
Sbjct: 5 DEVSAIVVDLGSHTCKAGYAGEDAPKAVFPSV 36
Score = 29.9 bits (64), Expect = 4.6
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALNXKAFXEXXDT*LLFE 545
WD + I + F + L + EHP LL E LN + E L+FE
Sbjct: 103 WDLVDNIWEHAFKSCLMIDPTEHPMLLAEPPLNTQQQREKAAE-LMFE 149
>At3g27000.1 68416.m03378 actin-related protein 2 (ARP2) nearly
identical to actin-related protein 2 (ARP2) [Arabidopsis
thaliana] GI:3818624; contains Pfam profile PF00022:
Actin
Length = 389
Score = 31.1 bits (67), Expect = 2.0
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +3
Query: 402 WDDXEXIXHYTFXNELXVATEEHPXLLTEAALN 500
WDD E + + F NEL + + LLT+ LN
Sbjct: 80 WDDMEHVWDHAFYNELKINPSDCKILLTDPPLN 112
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,648,576
Number of Sequences: 28952
Number of extensions: 118646
Number of successful extensions: 135
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4057847232
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -