BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_E03_e597_09.seq
(1535 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC15E1.03 |rpl36a||60S ribosomal protein L36/L42|Schizosacchar... 122 1e-28
SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity fac... 27 6.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 9.1
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 9.1
>SPAC15E1.03 |rpl36a||60S ribosomal protein
L36/L42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 106
Score = 122 bits (294), Expect = 1e-28
Identities = 56/106 (52%), Positives = 69/106 (65%), Gaps = 2/106 (1%)
Frame = +3
Query: 93 MVNVPKQRRTY--XXXXXXXXXXXXSQYKKSKERHAAQGRRRYDRKQQGYGGQSKPIFXX 266
MVN+PK R+TY +QYKK + AQG+RRYDRKQ G+GGQ+KP+F
Sbjct: 1 MVNIPKTRKTYCPGKNCRKHTVHRVTQYKKGPDSKLAQGKRRYDRKQSGFGGQTKPVFHK 60
Query: 267 XXXXXXXIVLRLECADCKVRSQVALKRCKHFELGGDKKRKGQMIQF 404
+VLRLEC CK ++Q+ LKRCKHFELGG+KK KG IQF
Sbjct: 61 KAKVTKKVVLRLECVSCKYKNQLVLKRCKHFELGGEKKTKGAAIQF 106
>SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity factor
complex subunit, Fip1 homolog |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 344
Score = 27.1 bits (57), Expect = 6.9
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -3
Query: 387 PSSSCHHQAQSAYNASGPPVTSPCSLHIP 301
P+SS + A + YNAS PP P S + P
Sbjct: 287 PTSSYGNGASTNYNASRPPSNHPHSSNYP 315
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 26.6 bits (56), Expect = 9.1
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
Frame = +1
Query: 472 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 636
P P+ S ++ H + + +L N I+L ++PLSP A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231
Query: 637 SCA 645
S A
Sbjct: 232 SDA 234
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 26.6 bits (56), Expect = 9.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +3
Query: 579 PPFASWRNSEEARTDRPSQQLRSLN 653
PP AS RN E + PS+Q S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,112,719
Number of Sequences: 5004
Number of extensions: 69997
Number of successful extensions: 121
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 862245690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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