Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_D10_e652_08.seq
(1561 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 30 0.75
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 30 1.00
SPBC887.18c |||transcription adaptor protein |Schizosaccharomyce... 29 2.3
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 28 3.0
SPAC664.09 |ggt1||gamma-glutamyltranspeptidase Ggt1 |Schizosacch... 28 4.0
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 27 7.0
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 27 9.3
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 9.3
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 9.3
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 30.3 bits (65), Expect = 0.75
Identities = 15/39 (38%), Positives = 22/39 (56%)
Frame = -3
Query: 734 FMPSARTASDQVFYEIVFGTNFNSVPTERAFRWEQCETF 618
F+P R++S V Y +F + NS P +RW + ETF
Sbjct: 174 FVPPKRSSSSCVTYVCLF-LDSNSNPRINVYRWSKTETF 211
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 29.9 bits (64), Expect = 1.00
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +1
Query: 481 IKTILQDFHTRSLHRCLQQNSSILSSGLNFSQKQEPNTLFLPASIMKVSHCSHRNAR 651
+KTI D TR L L S + ++ ++P+T +L I + +HRNAR
Sbjct: 419 VKTISFDPLTRHFAHRLSAELGNLMSQILAAKDRDPSTSYLKTVIFPTNEQTHRNAR 475
>SPBC887.18c |||transcription adaptor protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 339
Score = 28.7 bits (61), Expect = 2.3
Identities = 22/55 (40%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Frame = +3
Query: 234 LVNAKKYNFDNLENSISEHDEVNSKKFNPDWKDLDTRKLPEWYDRAKI--GIFLH 392
L A K NFD L NSI H +K N + L LP W+ R K+ +FLH
Sbjct: 64 LTFAGKSNFDKLHNSIIFHILKLMQKNNDTFSAL--HHLP-WFKRKKVDNSLFLH 115
>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 2410
Score = 28.3 bits (60), Expect = 3.0
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = +3
Query: 342 RKLPEWYDRAKIGIFLHWGVYSVPSYG-SEWFW 437
R L +++ + IG++L W YS P G + W W
Sbjct: 2275 RWLQQFWATSNIGLYLPWAGYSGPYLGRTLWLW 2307
>SPAC664.09 |ggt1||gamma-glutamyltranspeptidase Ggt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 630
Score = 27.9 bits (59), Expect = 4.0
Identities = 17/53 (32%), Positives = 27/53 (50%)
Frame = -2
Query: 465 TSENHLSNYSKTILTHNWERNTHPSVKIFLF*LCRTIPVIFVCPNLSSPD*IF 307
+ HLS+ K+ + ER + PS+ + L TI +++ P L SPD F
Sbjct: 25 SGNRHLSSNKKSATSALEERASRPSILVTFLVLAGTILSLYIWPIL-SPDLFF 76
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 27.1 bits (57), Expect = 7.0
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = +1
Query: 475 LWIKTILQDFHTRSLHRCLQQNSSILSS 558
LWIK++ Q++ +++L L ++ + LSS
Sbjct: 475 LWIKSLRQEYESKNLLELLDKHQTALSS 502
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 26.6 bits (56), Expect = 9.3
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 5/47 (10%)
Frame = -1
Query: 328 FQSGLNFLEFTSSCSDIEFSRLSKLYFL-----AFTKYTSDNNMNIL 203
F L F EF+++ D E RLSKL + +++ +SD N IL
Sbjct: 1824 FDISLEFNEFSNNEDDSELERLSKLIKVPPLQELYSQMSSDENNQIL 1870
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 26.6 bits (56), Expect = 9.3
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +3
Query: 267 LENSISEHDEVNSKKFNPDWKDLDTRK 347
L N +++H+ + SK + K +DTRK
Sbjct: 1325 LRNKVADHESIRSKLSEVEMKLVDTRK 1351
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 26.6 bits (56), Expect = 9.3
Identities = 25/69 (36%), Positives = 34/69 (49%), Gaps = 9/69 (13%)
Frame = +1
Query: 481 IKTILQDFHTRSLHRCLQQNSSILS---SG---LNFSQKQEP---NTLFLPASIMKVSHC 633
I TIL+ ++R R LQQ S + S SG LNF Q +P N + K+
Sbjct: 1712 INTILRRNNSRGRRRMLQQMSPLKSNKFSGTNDLNFQQATKPDGSNKSSYMERLEKLKQN 1771
Query: 634 SHRNARSVG 660
S R+ +SVG
Sbjct: 1772 SERHLQSVG 1780
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,847,148
Number of Sequences: 5004
Number of extensions: 93889
Number of successful extensions: 233
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 233
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 878103082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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