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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030731E7_D08_e636_08.seq
         (1514 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac...    74   5e-14
SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo...    52   3e-07
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar...    48   3e-06
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k...    44   6e-05
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    36   0.015
SPAC2C4.14c |ppk11||PAK-related kinase Ppk11|Schizosaccharomyces...    33   0.14 
SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizo...    32   0.18 
SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac...    31   0.32 
SPBC1778.10c |ppk21|SPBC4C3.11|serine/threonine protein kinase P...    31   0.55 
SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|...    30   0.73 
SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz...    30   0.96 
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom...    29   1.3  
SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c...    29   1.3  
SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt...    29   2.2  
SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces po...    28   2.9  
SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosa...    28   2.9  
SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po...    28   2.9  
SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch...    27   6.8  
SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|...    27   6.8  
SPCC4G3.02 |aph1||bis|Schizosaccharomyces pombe|chr 3|||Manual         27   6.8  
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha...    27   9.0  

>SPCC777.14 |prp4||serine/threonine protein kinase
           Prp4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 477

 Score = 74.1 bits (174), Expect = 5e-14
 Identities = 45/153 (29%), Positives = 71/153 (46%), Gaps = 3/153 (1%)
 Frame = +3

Query: 273 KQTITDMFSEKDDFELNSLN--EKVNTNKEXSLQLTDNWDDVEGYYRINIGELINKKYTI 446
           + T  DMF++     +   N  + ++T       + DNWDD+EGYY++ + E ++ +Y +
Sbjct: 102 EDTEDDMFADSPSPSVKRQNTGKGISTLTRSFADMQDNWDDIEGYYKVVLMEELDSRYIV 161

Query: 447 KDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCDFERDMSCXPRKXXSL 626
           +  LG+G+F+ V  A D   N   AIKIIRN  ++Y          ER +    R+    
Sbjct: 162 QSNLGKGMFSTVVSALDRNRNQTFAIKIIRNNEVMYKEGLKEVSILER-LQAADREGKQH 220

Query: 627 XXGYXAFLTXRTPLXR-XEPLPXXLRCXPKKYG 722
              Y      +  L    E L   LR   KK+G
Sbjct: 221 IIHYERHFMHKNHLCMVFEMLSLNLRDILKKFG 253


>SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 534

 Score = 51.6 bits (118), Expect = 3e-07
 Identities = 27/63 (42%), Positives = 37/63 (58%), Gaps = 4/63 (6%)
 Frame = +3

Query: 381 WDDVEGYYRINIGELIN----KKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXL 548
           +D+V   Y + + +L+     +KY I DTLG G F  VAR QD +   IVAIK+I+N   
Sbjct: 107 FDNVNHDYVLYVNDLLGTDEGRKYLILDTLGHGTFGQVARCQDLKTQQIVAIKVIKNKPA 166

Query: 549 LYN 557
            YN
Sbjct: 167 FYN 169


>SPAC2F7.03c |pom1||DYRK family protein kinase
           Pom1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1087

 Score = 48.0 bits (109), Expect = 3e-06
 Identities = 22/53 (41%), Positives = 33/53 (62%)
 Frame = +3

Query: 381 WDDVEGYYRINIGELINKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRN 539
           +DD  G Y++ +G+ I  +Y + D LG+G F  V R  D +   +VA+KIIRN
Sbjct: 680 FDDERGDYKVVLGDHIAYRYEVVDFLGKGSFGQVLRCIDYETGKLVALKIIRN 732


>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
           kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 836

 Score = 44.0 bits (99), Expect = 6e-05
 Identities = 20/53 (37%), Positives = 33/53 (62%)
 Frame = +3

Query: 381 WDDVEGYYRINIGELINKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRN 539
           +DD  G Y+   G+ +  +Y I DT+G+G F  V +  D++   +VAIK+I+N
Sbjct: 499 FDDDNGDYKAIQGDHLLYRYEIIDTVGKGSFGQVLKCIDHKRGQVVAIKVIKN 551



 Score = 33.1 bits (72), Expect = 0.10
 Identities = 15/37 (40%), Positives = 19/37 (51%)
 Frame = +1

Query: 565 LXEXAILKEICXADPENXXHXXXXIXHFLXXGPLCXV 675
           L E  ILK +C ADP +  +    + HF   G LC V
Sbjct: 560 LVEVGILKRLCEADPADKNNVIRYLSHFDFRGHLCIV 596


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 35.9 bits (79), Expect = 0.015
 Identities = 16/52 (30%), Positives = 25/52 (48%)
 Frame = +3

Query: 381 WDDVEGYYRINIGELINKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536
           +DD +G+Y++        +YT+   LG G F  V +  D       AIK+ R
Sbjct: 343 FDDDDGHYKVVPNSKFANRYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTR 394


>SPAC2C4.14c |ppk11||PAK-related kinase Ppk11|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 312

 Score = 32.7 bits (71), Expect = 0.14
 Identities = 14/33 (42%), Positives = 22/33 (66%)
 Frame = +3

Query: 435 KYTIKDTLGQGVFANVARAQDNQENXIVAIKII 533
           +Y     +GQG F +V RAQD + + IVA+K++
Sbjct: 5   QYRDLQLIGQGSFGSVFRAQDVESSKIVALKVV 37


>SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase
           Srk1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 580

 Score = 32.3 bits (70), Expect = 0.18
 Identities = 13/35 (37%), Positives = 23/35 (65%)
 Frame = +3

Query: 432 KKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536
           ++YT+   +G G F+NV +A  N+    VAIK+++
Sbjct: 122 EQYTLLQKMGDGAFSNVYKAIHNRTGEKVAIKVVQ 156


>SPCC18B5.11c |cds1||replication checkpoint kinase
           Cds1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score = 31.5 bits (68), Expect = 0.32
 Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
 Frame = +3

Query: 429 NKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCD-FERDM 596
           N  Y I  TLG G FA V  A +       AIKII    +L  +   R  + F+R++
Sbjct: 164 NSHYEIIRTLGSGTFAVVKLAVEVNSGKWYAIKIINKRKILLTSSEKRATEMFQREI 220


>SPBC1778.10c |ppk21|SPBC4C3.11|serine/threonine protein kinase
           Ppk21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 550

 Score = 30.7 bits (66), Expect = 0.55
 Identities = 16/52 (30%), Positives = 26/52 (50%)
 Frame = +3

Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCDFERD 593
           Y   D +G G F+ V RA D +     AIK++    ++  N+  +  + ERD
Sbjct: 54  YVFGDIIGDGSFSKVRRATDKKSWKEYAIKVLDKKYIVKENK-VKYVNIERD 104


>SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase
           Pef1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 288

 Score = 30.3 bits (65), Expect = 0.73
 Identities = 13/33 (39%), Positives = 21/33 (63%)
 Frame = +3

Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536
           Y   + LG+G +A+V + Q+     IVA+K+IR
Sbjct: 3   YQRLEKLGEGTYAHVYKGQNRVTGEIVALKVIR 35


>SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase
           Cmk2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 504

 Score = 29.9 bits (64), Expect = 0.96
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = +3

Query: 432 KKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536
           +KY + + LG G F+ V +A        VA+K+IR
Sbjct: 63  EKYQLIENLGDGAFSQVYKAYSIDRKEHVAVKVIR 97


>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1016

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 13/41 (31%), Positives = 22/41 (53%)
 Frame = +3

Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNN 560
           +T    LG+G F  V  A+   E  + AIK+++   +L N+
Sbjct: 683 FTFLSVLGKGNFGKVMLAELKSEKQLYAIKVLKKEFILEND 723


>SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 683

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
 Frame = +3

Query: 429 NKK---YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXL 548
           NKK   YT  D +G+G FA   R +DN  N I A K+I    L
Sbjct: 35  NKKNIFYTRYDCIGEGGFARCFRVKDNYGN-IYAAKVIAKRSL 76


>SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic
           subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 512

 Score = 28.7 bits (61), Expect = 2.2
 Identities = 13/48 (27%), Positives = 19/48 (39%)
 Frame = +3

Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCD 581
           +    TLG G F  V   Q N      AIK++    ++   +    CD
Sbjct: 201 FNFLQTLGTGSFGRVHLVQSNHNRLYYAIKVLEKKKIVDMKQIEHTCD 248


>SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 471

 Score = 28.3 bits (60), Expect = 2.9
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = +3

Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKII 533
           YT+   LG G F  V +A++N    I+AIK I
Sbjct: 9   YTLLRKLGSGSFGVVWKARENVSGDIIAIKQI 40


>SPBC776.12c |hsk1||serine/threonine protein kinase
           Hsk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 507

 Score = 28.3 bits (60), Expect = 2.9
 Identities = 17/76 (22%), Positives = 38/76 (50%)
 Frame = +3

Query: 270 EKQTITDMFSEKDDFELNSLNEKVNTNKEXSLQLTDNWDDVEGYYRINIGELINKKYTIK 449
           E+QT  D   E  + +  ++NE  +      +   D  +++E   R  +   + + Y + 
Sbjct: 15  EQQTDIDSECEITEVDDENVNENKSQEMIQDIPARDR-EEIENITRTFVE--LQENYRLI 71

Query: 450 DTLGQGVFANVARAQD 497
           + +G+G F++V +A+D
Sbjct: 72  EKIGEGTFSSVYKAED 87


>SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 28.3 bits (60), Expect = 2.9
 Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
 Frame = +3

Query: 285 TDMFSEKDDFELNSLNEKVNTNKEXSLQ-LTDNWDDVEGYYRINIGEL 425
           TD+  E+    LN LN+ V +    +LQ +T N  + +  YR +I EL
Sbjct: 132 TDILPEEVQLVLNDLNDDVKSANSANLQPITTNVVNEKDAYRKDIDEL 179


>SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1316

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 12/42 (28%), Positives = 21/42 (50%)
 Frame = +3

Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNR 563
           Y I   + +G F  V  ++ N    I AIK++R   ++  N+
Sbjct: 564 YEIIKPISKGTFGTVYLSRKNTTGEIYAIKVLRKVDMISKNQ 605


>SPBC30B4.02c |||R3H and G-patch domain, unknown biological
           role|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 17/56 (30%), Positives = 29/56 (51%)
 Frame = +3

Query: 285 TDMFSEKDDFELNSLNEKVNTNKEXSLQLTDNWDDVEGYYRINIGELINKKYTIKD 452
           TD  +E +D   + L E  + ++E  L + D  DD E    ++IG+ +   +TI D
Sbjct: 334 TDSLAEDED---DILEEDEDEDEEELLAIFDASDDSEIEAYLSIGDTLQNDFTIYD 386


>SPCC4G3.02 |aph1||bis|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 182

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 11/33 (33%), Positives = 18/33 (54%)
 Frame = -1

Query: 476 SKNPLTQSIFYSIFLVNKFTNIYPIISFHIIPV 378
           SK P+   +FY   L   F N+ PI+  H++ +
Sbjct: 8   SKFPVGSQVFYRTKLSAAFVNLKPILPGHVLVI 40


>SPAC13G7.04c |mac1||membrane anchored protein Mac1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 756

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = -1

Query: 452 IFYSIFLVNKFTNIYPIISFHIIPVIS 372
           +F+SIFLVN+  NI  II   I  +++
Sbjct: 104 VFFSIFLVNQAVNIINIIVVFITTLLT 130


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,707,900
Number of Sequences: 5004
Number of extensions: 53319
Number of successful extensions: 183
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 848370472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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