BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_D08_e636_08.seq (1514 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac... 74 5e-14 SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo... 52 3e-07 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 48 3e-06 SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 44 6e-05 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 36 0.015 SPAC2C4.14c |ppk11||PAK-related kinase Ppk11|Schizosaccharomyces... 33 0.14 SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizo... 32 0.18 SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac... 31 0.32 SPBC1778.10c |ppk21|SPBC4C3.11|serine/threonine protein kinase P... 31 0.55 SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|... 30 0.73 SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 30 0.96 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 29 1.3 SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 29 1.3 SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt... 29 2.2 SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces po... 28 2.9 SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosa... 28 2.9 SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po... 28 2.9 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 27 6.8 SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 27 6.8 SPCC4G3.02 |aph1||bis|Schizosaccharomyces pombe|chr 3|||Manual 27 6.8 SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 27 9.0 >SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosaccharomyces pombe|chr 3|||Manual Length = 477 Score = 74.1 bits (174), Expect = 5e-14 Identities = 45/153 (29%), Positives = 71/153 (46%), Gaps = 3/153 (1%) Frame = +3 Query: 273 KQTITDMFSEKDDFELNSLN--EKVNTNKEXSLQLTDNWDDVEGYYRINIGELINKKYTI 446 + T DMF++ + N + ++T + DNWDD+EGYY++ + E ++ +Y + Sbjct: 102 EDTEDDMFADSPSPSVKRQNTGKGISTLTRSFADMQDNWDDIEGYYKVVLMEELDSRYIV 161 Query: 447 KDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCDFERDMSCXPRKXXSL 626 + LG+G+F+ V A D N AIKIIRN ++Y ER + R+ Sbjct: 162 QSNLGKGMFSTVVSALDRNRNQTFAIKIIRNNEVMYKEGLKEVSILER-LQAADREGKQH 220 Query: 627 XXGYXAFLTXRTPLXR-XEPLPXXLRCXPKKYG 722 Y + L E L LR KK+G Sbjct: 221 IIHYERHFMHKNHLCMVFEMLSLNLRDILKKFG 253 >SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizosaccharomyces pombe|chr 1|||Manual Length = 534 Score = 51.6 bits (118), Expect = 3e-07 Identities = 27/63 (42%), Positives = 37/63 (58%), Gaps = 4/63 (6%) Frame = +3 Query: 381 WDDVEGYYRINIGELIN----KKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXL 548 +D+V Y + + +L+ +KY I DTLG G F VAR QD + IVAIK+I+N Sbjct: 107 FDNVNHDYVLYVNDLLGTDEGRKYLILDTLGHGTFGQVARCQDLKTQQIVAIKVIKNKPA 166 Query: 549 LYN 557 YN Sbjct: 167 FYN 169 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 48.0 bits (109), Expect = 3e-06 Identities = 22/53 (41%), Positives = 33/53 (62%) Frame = +3 Query: 381 WDDVEGYYRINIGELINKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRN 539 +DD G Y++ +G+ I +Y + D LG+G F V R D + +VA+KIIRN Sbjct: 680 FDDERGDYKVVLGDHIAYRYEVVDFLGKGSFGQVLRCIDYETGKLVALKIIRN 732 >SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 836 Score = 44.0 bits (99), Expect = 6e-05 Identities = 20/53 (37%), Positives = 33/53 (62%) Frame = +3 Query: 381 WDDVEGYYRINIGELINKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRN 539 +DD G Y+ G+ + +Y I DT+G+G F V + D++ +VAIK+I+N Sbjct: 499 FDDDNGDYKAIQGDHLLYRYEIIDTVGKGSFGQVLKCIDHKRGQVVAIKVIKN 551 Score = 33.1 bits (72), Expect = 0.10 Identities = 15/37 (40%), Positives = 19/37 (51%) Frame = +1 Query: 565 LXEXAILKEICXADPENXXHXXXXIXHFLXXGPLCXV 675 L E ILK +C ADP + + + HF G LC V Sbjct: 560 LVEVGILKRLCEADPADKNNVIRYLSHFDFRGHLCIV 596 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 35.9 bits (79), Expect = 0.015 Identities = 16/52 (30%), Positives = 25/52 (48%) Frame = +3 Query: 381 WDDVEGYYRINIGELINKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536 +DD +G+Y++ +YT+ LG G F V + D AIK+ R Sbjct: 343 FDDDDGHYKVVPNSKFANRYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTR 394 >SPAC2C4.14c |ppk11||PAK-related kinase Ppk11|Schizosaccharomyces pombe|chr 1|||Manual Length = 312 Score = 32.7 bits (71), Expect = 0.14 Identities = 14/33 (42%), Positives = 22/33 (66%) Frame = +3 Query: 435 KYTIKDTLGQGVFANVARAQDNQENXIVAIKII 533 +Y +GQG F +V RAQD + + IVA+K++ Sbjct: 5 QYRDLQLIGQGSFGSVFRAQDVESSKIVALKVV 37 >SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 32.3 bits (70), Expect = 0.18 Identities = 13/35 (37%), Positives = 23/35 (65%) Frame = +3 Query: 432 KKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536 ++YT+ +G G F+NV +A N+ VAIK+++ Sbjct: 122 EQYTLLQKMGDGAFSNVYKAIHNRTGEKVAIKVVQ 156 >SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 31.5 bits (68), Expect = 0.32 Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 1/57 (1%) Frame = +3 Query: 429 NKKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCD-FERDM 596 N Y I TLG G FA V A + AIKII +L + R + F+R++ Sbjct: 164 NSHYEIIRTLGSGTFAVVKLAVEVNSGKWYAIKIINKRKILLTSSEKRATEMFQREI 220 >SPBC1778.10c |ppk21|SPBC4C3.11|serine/threonine protein kinase Ppk21|Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 30.7 bits (66), Expect = 0.55 Identities = 16/52 (30%), Positives = 26/52 (50%) Frame = +3 Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCDFERD 593 Y D +G G F+ V RA D + AIK++ ++ N+ + + ERD Sbjct: 54 YVFGDIIGDGSFSKVRRATDKKSWKEYAIKVLDKKYIVKENK-VKYVNIERD 104 >SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|Schizosaccharomyces pombe|chr 3|||Manual Length = 288 Score = 30.3 bits (65), Expect = 0.73 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = +3 Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536 Y + LG+G +A+V + Q+ IVA+K+IR Sbjct: 3 YQRLEKLGEGTYAHVYKGQNRVTGEIVALKVIR 35 >SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 29.9 bits (64), Expect = 0.96 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +3 Query: 432 KKYTIKDTLGQGVFANVARAQDNQENXIVAIKIIR 536 +KY + + LG G F+ V +A VA+K+IR Sbjct: 63 EKYQLIENLGDGAFSQVYKAYSIDRKEHVAVKVIR 97 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 29.5 bits (63), Expect = 1.3 Identities = 13/41 (31%), Positives = 22/41 (53%) Frame = +3 Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNN 560 +T LG+G F V A+ E + AIK+++ +L N+ Sbjct: 683 FTFLSVLGKGNFGKVMLAELKSEKQLYAIKVLKKEFILEND 723 >SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr 1|||Manual Length = 683 Score = 29.5 bits (63), Expect = 1.3 Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 3/43 (6%) Frame = +3 Query: 429 NKK---YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXL 548 NKK YT D +G+G FA R +DN N I A K+I L Sbjct: 35 NKKNIFYTRYDCIGEGGFARCFRVKDNYGN-IYAAKVIAKRSL 76 >SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 512 Score = 28.7 bits (61), Expect = 2.2 Identities = 13/48 (27%), Positives = 19/48 (39%) Frame = +3 Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNRXXRXCD 581 + TLG G F V Q N AIK++ ++ + CD Sbjct: 201 FNFLQTLGTGSFGRVHLVQSNHNRLYYAIKVLEKKKIVDMKQIEHTCD 248 >SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces pombe|chr 1|||Manual Length = 471 Score = 28.3 bits (60), Expect = 2.9 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = +3 Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKII 533 YT+ LG G F V +A++N I+AIK I Sbjct: 9 YTLLRKLGSGSFGVVWKARENVSGDIIAIKQI 40 >SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 28.3 bits (60), Expect = 2.9 Identities = 17/76 (22%), Positives = 38/76 (50%) Frame = +3 Query: 270 EKQTITDMFSEKDDFELNSLNEKVNTNKEXSLQLTDNWDDVEGYYRINIGELINKKYTIK 449 E+QT D E + + ++NE + + D +++E R + + + Y + Sbjct: 15 EQQTDIDSECEITEVDDENVNENKSQEMIQDIPARDR-EEIENITRTFVE--LQENYRLI 71 Query: 450 DTLGQGVFANVARAQD 497 + +G+G F++V +A+D Sbjct: 72 EKIGEGTFSSVYKAED 87 >SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 28.3 bits (60), Expect = 2.9 Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 1/48 (2%) Frame = +3 Query: 285 TDMFSEKDDFELNSLNEKVNTNKEXSLQ-LTDNWDDVEGYYRINIGEL 425 TD+ E+ LN LN+ V + +LQ +T N + + YR +I EL Sbjct: 132 TDILPEEVQLVLNDLNDDVKSANSANLQPITTNVVNEKDAYRKDIDEL 179 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 27.1 bits (57), Expect = 6.8 Identities = 12/42 (28%), Positives = 21/42 (50%) Frame = +3 Query: 438 YTIKDTLGQGVFANVARAQDNQENXIVAIKIIRNXXLLYNNR 563 Y I + +G F V ++ N I AIK++R ++ N+ Sbjct: 564 YEIIKPISKGTFGTVYLSRKNTTGEIYAIKVLRKVDMISKNQ 605 >SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 27.1 bits (57), Expect = 6.8 Identities = 17/56 (30%), Positives = 29/56 (51%) Frame = +3 Query: 285 TDMFSEKDDFELNSLNEKVNTNKEXSLQLTDNWDDVEGYYRINIGELINKKYTIKD 452 TD +E +D + L E + ++E L + D DD E ++IG+ + +TI D Sbjct: 334 TDSLAEDED---DILEEDEDEDEEELLAIFDASDDSEIEAYLSIGDTLQNDFTIYD 386 >SPCC4G3.02 |aph1||bis|Schizosaccharomyces pombe|chr 3|||Manual Length = 182 Score = 27.1 bits (57), Expect = 6.8 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = -1 Query: 476 SKNPLTQSIFYSIFLVNKFTNIYPIISFHIIPV 378 SK P+ +FY L F N+ PI+ H++ + Sbjct: 8 SKFPVGSQVFYRTKLSAAFVNLKPILPGHVLVI 40 >SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 756 Score = 26.6 bits (56), Expect = 9.0 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = -1 Query: 452 IFYSIFLVNKFTNIYPIISFHIIPVIS 372 +F+SIFLVN+ NI II I +++ Sbjct: 104 VFFSIFLVNQAVNIINIIVVFITTLLT 130 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,707,900 Number of Sequences: 5004 Number of extensions: 53319 Number of successful extensions: 183 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 164 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 848370472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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