Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_D07_e628_07.seq
(1515 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 220 4e-58
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 126 1e-29
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 123 7e-29
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 104 4e-23
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 75 3e-14
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 67 7e-12
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 66 9e-12
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 49 2e-06
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.32
SPBC2D10.07c |||mitochondrial inner membrane peptidase complex c... 30 0.96
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 28 2.9
SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA synthase|Sc... 27 9.0
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 220 bits (538), Expect = 4e-58
Identities = 101/108 (93%), Positives = 103/108 (95%)
Frame = +3
Query: 309 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 488
MGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLT
Sbjct: 47 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLT 106
Query: 489 EAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
EAPLNPK+NREKMTQI+FETFN PA YVA QAVLSLYASGRTTGIVLD
Sbjct: 107 EAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLD 154
Score = 52.4 bits (120), Expect = 2e-07
Identities = 28/62 (45%), Positives = 33/62 (53%)
Frame = +1
Query: 634 SGDGVSHTVPIYEGYXLTGRHSXVXTXXGPEPXXLPWMXIXXKRGYXFXXXXXREIVXEX 813
SGDGV+HTVPIYEGY L H+ + M I +RGY F REIV +
Sbjct: 155 SGDGVTHTVPIYEGYALP--HAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDI 212
Query: 814 KE 819
KE
Sbjct: 213 KE 214
Score = 50.8 bits (116), Expect = 5e-07
Identities = 22/30 (73%), Positives = 27/30 (90%)
Frame = +1
Query: 172 DEEVAALVVDNGSGMCKAGFAGDXRSPRRI 261
+EE+AALV+DNGSGMCKAGFAGD +PR +
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDD-APRAV 30
Score = 44.0 bits (99), Expect = 6e-05
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = +2
Query: 245 APRAVFPSIVGRPRHQGVMVLHGSE 319
APRAVFPSIVGRPRH G+MV G +
Sbjct: 26 APRAVFPSIVGRPRHHGIMVGMGQK 50
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 126 bits (303), Expect = 1e-29
Identities = 61/130 (46%), Positives = 83/130 (63%), Gaps = 1/130 (0%)
Frame = +3
Query: 246 LPAPYFPRSWGDLVTRASWSCMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKI 425
+P FP G + QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+I
Sbjct: 30 IPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEI 89
Query: 426 WHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYA 602
W + + ++ L PEEHP+LLTE PL N+EK+ + +ET N PA+ + Q VL+LYA
Sbjct: 90 WSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYA 149
Query: 603 SGRTTGIVLD 632
S RTTGIVL+
Sbjct: 150 SARTTGIVLE 159
Score = 31.5 bits (68), Expect = 0.32
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +1
Query: 190 LVVDNGSGMCKAGFAGD 240
+ +DNGSG KAGFAGD
Sbjct: 12 ICIDNGSGFIKAGFAGD 28
Score = 31.5 bits (68), Expect = 0.32
Identities = 18/61 (29%), Positives = 27/61 (44%)
Frame = +1
Query: 637 GDGVSHTVPIYEGYXLTGRHSXVXTXXGPEPXXLPWMXIXXKRGYXFXXXXXREIVXEXK 816
GDG++H+VPIY+G+ + + G K G+ +EIV E K
Sbjct: 161 GDGLTHSVPIYDGFSIPS--AIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIK 218
Query: 817 E 819
E
Sbjct: 219 E 219
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 123 bits (296), Expect = 7e-29
Identities = 52/105 (49%), Positives = 79/105 (75%)
Frame = +3
Query: 318 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 497
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 498 LNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
+NP ANREKM + MFE + +YVA QAVLSLYA G ++G+V+D
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVD 154
Score = 34.7 bits (76), Expect = 0.034
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +1
Query: 634 SGDGVSHTVPIYEGYXLTGRHSXVXTXXGPEPXXLPWMXIXXKRGYXFXXXXXREIVXEX 813
SGDGV+H VP+YE L H + + ++GY F E V E
Sbjct: 155 SGDGVTHIVPVYESVVL--NHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREM 212
Query: 814 KE 819
KE
Sbjct: 213 KE 214
Score = 27.9 bits (59), Expect = 3.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 178 EVAALVVDNGSGMCKAGFAGD 240
E A +V+DNG+G K G+A D
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKD 22
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 104 bits (249), Expect = 4e-23
Identities = 47/96 (48%), Positives = 62/96 (64%), Gaps = 1/96 (1%)
Frame = +3
Query: 321 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 497
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 498 LNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYAS 605
LNP NRE +IMFE+FN +Y+A QAVL+L AS
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAAS 168
Score = 29.9 bits (64), Expect = 0.96
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1
Query: 634 SGDGVSHTVPIYEGYXL 684
SGDGV+H +P+ EGY +
Sbjct: 186 SGDGVTHIIPVAEGYVI 202
Score = 27.1 bits (57), Expect = 6.8
Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +1
Query: 190 LVVDNGSGMCKAGFAGDXRSPRRISLDRGATSSPG-RHGPA 309
+++DNG+G K G+AG+ +P + AT S G GPA
Sbjct: 8 IIMDNGTGYSKLGYAGND-APSYVFPTVIATRSAGASSGPA 47
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 74.9 bits (176), Expect = 3e-14
Identities = 34/90 (37%), Positives = 51/90 (56%)
Frame = +3
Query: 363 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 542
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 543 ETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
E+ PA Y+A Q + +ASG+ T ++D
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVD 152
Score = 27.1 bits (57), Expect = 6.8
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +1
Query: 175 EEVAALVVDNGSGMCKAGFAGD 240
+EV+A+V+D GS + GF+G+
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGE 30
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 66.9 bits (156), Expect = 7e-12
Identities = 36/121 (29%), Positives = 69/121 (57%), Gaps = 3/121 (2%)
Frame = +3
Query: 279 DLVTRASWSCMGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELR 455
+LV+R + + + VG++ + G ++ + P E +++NWD ME++ +TF +L
Sbjct: 52 NLVSRYRDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLG 110
Query: 456 VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGR--TTGIVL 629
+ EHP+ +TE NP R MT+++FE +N P++ + S Y + + ++GIVL
Sbjct: 111 IDRMEHPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVL 170
Query: 630 D 632
+
Sbjct: 171 N 171
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 66.5 bits (155), Expect = 9e-12
Identities = 31/107 (28%), Positives = 60/107 (56%), Gaps = 2/107 (1%)
Frame = +3
Query: 318 KDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 491
++ YV DE Q I + +K +GI+ +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 492 APLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVD 155
Score = 26.6 bits (56), Expect = 9.0
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = +1
Query: 175 EEVAALVVDNGSGMCKAGFAGDXRSPRRI 261
EE+ +LV+D GS + G+AG+ SP I
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGE-ESPMTI 35
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 48.8 bits (111), Expect = 2e-06
Identities = 28/89 (31%), Positives = 43/89 (48%), Gaps = 4/89 (4%)
Frame = +3
Query: 378 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 545
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 546 TFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
P +A + ++ LYA G G+V+D
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVID 200
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.5 bits (68), Expect = 0.32
Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
Frame = +3
Query: 438 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTP 560
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPBC2D10.07c |||mitochondrial inner membrane peptidase complex
catalytic subunit|Schizosaccharomyces pombe|chr
2|||Manual
Length = 157
Score = 29.9 bits (64), Expect = 0.96
Identities = 18/66 (27%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +1
Query: 466 RSTQSCSP-KLPSTPKPTERR*HRLCSRPSTHPPCT*LXRPCSHCTRPVVPPVSCWISGD 642
R +SCS + + KP++ + H +C R P T P S + +P W++GD
Sbjct: 55 RFARSCSVGDVVVSAKPSDSKQH-VCKRIIGMPGDTIYVDPTSSNKKITIPLGHVWLAGD 113
Query: 643 GVSHTV 660
++H++
Sbjct: 114 NIAHSL 119
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 28.3 bits (60), Expect = 2.9
Identities = 14/50 (28%), Positives = 22/50 (44%)
Frame = +2
Query: 593 TVRVRSYHRYRAGFPAMVYPTQYPSTRXTPLPDAIPXXXLXPVPNLTXYP 742
++R+ S HR R+ P P + PS +P +P P P +P
Sbjct: 338 SLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLPPADGVPKPTPDGFP 387
>SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA
synthase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 447
Score = 26.6 bits (56), Expect = 9.0
Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
Frame = +3
Query: 219 QSRFRGRRALPAPYFPRSWGDLVTRASWSCMGQKDSYV-GDEAQSKR 356
+ RF +R P+ Y P + G++ T + +SC+ S V DE + KR
Sbjct: 308 KERFN-KRVSPSVYAPTNCGNMYTASIFSCLTALLSRVPADELKGKR 353
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,329,721
Number of Sequences: 5004
Number of extensions: 79109
Number of successful extensions: 194
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 848370472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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