Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_D07_e628_07.seq
(1515 letters)
Database: mosquito
2352 sequences; 563,979 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
U02964-1|AAA03444.1| 376|Anopheles gambiae actin 1D protein. 231 4e-62
U02933-1|AAA56882.1| 376|Anopheles gambiae actin 1D protein. 231 4e-62
U02930-1|AAA56881.1| 376|Anopheles gambiae actin 1D protein. 231 4e-62
CR954256-1|CAJ14142.1| 376|Anopheles gambiae actin protein. 219 2e-58
AF444783-1|AAL37904.1| 1356|Anopheles gambiae Trex protein. 25 5.7
>U02964-1|AAA03444.1| 376|Anopheles gambiae actin 1D protein.
Length = 376
Score = 231 bits (565), Expect = 4e-62
Identities = 107/108 (99%), Positives = 107/108 (99%)
Frame = +3
Query: 309 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 488
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
Sbjct: 48 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 107
Query: 489 EAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
EAPLNPKANREKMTQIMFETFNTPAMYVA QAVLSLYASGRTTGIVLD
Sbjct: 108 EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLD 155
Score = 58.8 bits (136), Expect = 4e-10
Identities = 27/32 (84%), Positives = 29/32 (90%)
Frame = +1
Query: 166 MCDEEVAALVVDNGSGMCKAGFAGDXRSPRRI 261
MCDEEVAALVVDNGSGMCKAGFAGD +PR +
Sbjct: 1 MCDEEVAALVVDNGSGMCKAGFAGDD-APRAV 31
Score = 54.0 bits (124), Expect = 1e-08
Identities = 29/62 (46%), Positives = 33/62 (53%)
Frame = +1
Query: 634 SGDGVSHTVPIYEGYXLTGRHSXVXTXXGPEPXXLPWMXIXXKRGYXFXXXXXREIVXEX 813
SGDGVSHTVPIYEGY L H+ + M I +RGY F REIV +
Sbjct: 156 SGDGVSHTVPIYEGYALP--HAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213
Query: 814 KE 819
KE
Sbjct: 214 KE 215
Score = 46.4 bits (105), Expect = 2e-06
Identities = 21/25 (84%), Positives = 22/25 (88%)
Frame = +2
Query: 245 APRAVFPSIVGRPRHQGVMVLHGSE 319
APRAVFPSIVGRPRHQGVMV G +
Sbjct: 27 APRAVFPSIVGRPRHQGVMVGMGQK 51
>U02933-1|AAA56882.1| 376|Anopheles gambiae actin 1D protein.
Length = 376
Score = 231 bits (565), Expect = 4e-62
Identities = 107/108 (99%), Positives = 107/108 (99%)
Frame = +3
Query: 309 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 488
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
Sbjct: 48 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 107
Query: 489 EAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
EAPLNPKANREKMTQIMFETFNTPAMYVA QAVLSLYASGRTTGIVLD
Sbjct: 108 EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLD 155
Score = 58.8 bits (136), Expect = 4e-10
Identities = 27/32 (84%), Positives = 29/32 (90%)
Frame = +1
Query: 166 MCDEEVAALVVDNGSGMCKAGFAGDXRSPRRI 261
MCDEEVAALVVDNGSGMCKAGFAGD +PR +
Sbjct: 1 MCDEEVAALVVDNGSGMCKAGFAGDD-APRAV 31
Score = 54.0 bits (124), Expect = 1e-08
Identities = 29/62 (46%), Positives = 33/62 (53%)
Frame = +1
Query: 634 SGDGVSHTVPIYEGYXLTGRHSXVXTXXGPEPXXLPWMXIXXKRGYXFXXXXXREIVXEX 813
SGDGVSHTVPIYEGY L H+ + M I +RGY F REIV +
Sbjct: 156 SGDGVSHTVPIYEGYALP--HAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213
Query: 814 KE 819
KE
Sbjct: 214 KE 215
Score = 46.4 bits (105), Expect = 2e-06
Identities = 21/25 (84%), Positives = 22/25 (88%)
Frame = +2
Query: 245 APRAVFPSIVGRPRHQGVMVLHGSE 319
APRAVFPSIVGRPRHQGVMV G +
Sbjct: 27 APRAVFPSIVGRPRHQGVMVGMGQK 51
>U02930-1|AAA56881.1| 376|Anopheles gambiae actin 1D protein.
Length = 376
Score = 231 bits (565), Expect = 4e-62
Identities = 107/108 (99%), Positives = 107/108 (99%)
Frame = +3
Query: 309 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 488
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
Sbjct: 48 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 107
Query: 489 EAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
EAPLNPKANREKMTQIMFETFNTPAMYVA QAVLSLYASGRTTGIVLD
Sbjct: 108 EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLD 155
Score = 58.8 bits (136), Expect = 4e-10
Identities = 27/32 (84%), Positives = 29/32 (90%)
Frame = +1
Query: 166 MCDEEVAALVVDNGSGMCKAGFAGDXRSPRRI 261
MCDEEVAALVVDNGSGMCKAGFAGD +PR +
Sbjct: 1 MCDEEVAALVVDNGSGMCKAGFAGDD-APRAV 31
Score = 54.0 bits (124), Expect = 1e-08
Identities = 29/62 (46%), Positives = 33/62 (53%)
Frame = +1
Query: 634 SGDGVSHTVPIYEGYXLTGRHSXVXTXXGPEPXXLPWMXIXXKRGYXFXXXXXREIVXEX 813
SGDGVSHTVPIYEGY L H+ + M I +RGY F REIV +
Sbjct: 156 SGDGVSHTVPIYEGYALP--HAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213
Query: 814 KE 819
KE
Sbjct: 214 KE 215
Score = 46.4 bits (105), Expect = 2e-06
Identities = 21/25 (84%), Positives = 22/25 (88%)
Frame = +2
Query: 245 APRAVFPSIVGRPRHQGVMVLHGSE 319
APRAVFPSIVGRPRHQGVMV G +
Sbjct: 27 APRAVFPSIVGRPRHQGVMVGMGQK 51
>CR954256-1|CAJ14142.1| 376|Anopheles gambiae actin protein.
Length = 376
Score = 219 bits (535), Expect = 2e-58
Identities = 99/108 (91%), Positives = 104/108 (96%)
Frame = +3
Query: 309 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 488
MG KD+YVGDEAQSKRGILTLKYPIEHGI+TNWDDMEKIWHHTFYNELRVAPEEHPVLLT
Sbjct: 48 MGNKDAYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLT 107
Query: 489 EAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYASGRTTGIVLD 632
EAPLNPK+NREKMTQIMFETF PA+YVA QAVLSLYASGRTTG+VLD
Sbjct: 108 EAPLNPKSNREKMTQIMFETFAAPAVYVAIQAVLSLYASGRTTGVVLD 155
Score = 54.0 bits (124), Expect = 1e-08
Identities = 29/62 (46%), Positives = 33/62 (53%)
Frame = +1
Query: 634 SGDGVSHTVPIYEGYXLTGRHSXVXTXXGPEPXXLPWMXIXXKRGYXFXXXXXREIVXEX 813
SGDGVSHTVPIYEGY L H+ + M I +RGY F REIV +
Sbjct: 156 SGDGVSHTVPIYEGYALP--HAILRMDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213
Query: 814 KE 819
KE
Sbjct: 214 KE 215
Score = 53.2 bits (122), Expect = 2e-08
Identities = 23/32 (71%), Positives = 27/32 (84%)
Frame = +1
Query: 166 MCDEEVAALVVDNGSGMCKAGFAGDXRSPRRI 261
MCD++ ALVVDNGSGMCKAGFAGD +PR +
Sbjct: 1 MCDDDAGALVVDNGSGMCKAGFAGDD-APRAV 31
Score = 46.8 bits (106), Expect = 2e-06
Identities = 21/25 (84%), Positives = 23/25 (92%)
Frame = +2
Query: 245 APRAVFPSIVGRPRHQGVMVLHGSE 319
APRAVFPSIVGRPRHQGVMV G++
Sbjct: 27 APRAVFPSIVGRPRHQGVMVGMGNK 51
>AF444783-1|AAL37904.1| 1356|Anopheles gambiae Trex protein.
Length = 1356
Score = 25.0 bits (52), Expect = 5.7
Identities = 12/38 (31%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -3
Query: 310 MQDHDALVTRSPHDRGK-YGAGSARLPRNRLCTFRTHC 200
+ D D++ + ++RG+ Y LP LC + THC
Sbjct: 703 LMDLDSIYCKLLYNRGRTYVPLVEALPNQFLCKYDTHC 740
Database: mosquito
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 563,979
Number of sequences in database: 2352
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,182,214
Number of Sequences: 2352
Number of extensions: 23833
Number of successful extensions: 63
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 58
length of database: 563,979
effective HSP length: 67
effective length of database: 406,395
effective search space used: 177594615
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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