BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_D04_e604_08.seq (1529 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4A8.15c |cdc3||profilin|Schizosaccharomyces pombe|chr 1|||Ma... 101 2e-22 SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 34 0.060 SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyce... 31 0.32 SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex ... 28 3.9 SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 27 5.2 SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomy... 27 6.9 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 27 6.9 >SPAC4A8.15c |cdc3||profilin|Schizosaccharomyces pombe|chr 1|||Manual Length = 127 Score = 101 bits (243), Expect = 2e-22 Identities = 49/127 (38%), Positives = 73/127 (57%), Gaps = 1/127 (0%) Frame = +3 Query: 150 MSWQDYVDKQLMASRCVSKAAIAGHDGN-VWAKSEGFEISKDEVAKIVAGFENESLLTSG 326 MSWQ YVD L+ + + +AAI G+ VWA S GF +S E+ + AGF++ + Sbjct: 1 MSWQAYVDTSLLGTGKIDRAAIVSRAGDSVWAASAGFNLSPQEIQGLAAGFQDPPSMFGT 60 Query: 327 GVTIAGTRYIYLSGTERIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGD 506 G+ +AG +YI + R I KL K G+ C+ T+ +++S Y E P +AA + E L D Sbjct: 61 GIILAGQKYITIRAEGRSIYGKLQKEGIICVATKLCILVSHYPETTLPGEAAKITEALAD 120 Query: 507 YLITCGY 527 YL+ GY Sbjct: 121 YLVGVGY 127 >SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1328 Score = 33.9 bits (74), Expect = 0.060 Identities = 17/48 (35%), Positives = 28/48 (58%), Gaps = 1/48 (2%) Frame = -2 Query: 835 LVEKSKXKFKNTNLNCTIQXQLLRG-SGERTKRVQNPQSNRDYTYVTQ 695 L+ KSK KF N + T Q +RG G+R K +++ + NR + ++ Q Sbjct: 1273 LIHKSKSKFSKGNHHSTNGTQSIRGRGGKRGKPLRSKELNRKHDHIVQ 1320 >SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyces pombe|chr 1|||Manual Length = 469 Score = 31.5 bits (68), Expect = 0.32 Identities = 14/31 (45%), Positives = 19/31 (61%) Frame = +2 Query: 404 RRALHEDTASRRNFSL*RTHSTPAGRIRSRE 496 +R+LH+ T S L RT+S P +RSRE Sbjct: 426 KRSLHDSTTSHNKSDLMRTNSDPQSAMRSRE 456 >SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex subunit Orp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 27.9 bits (59), Expect = 3.9 Identities = 13/40 (32%), Positives = 24/40 (60%) Frame = -3 Query: 312 VIHSQNRQLSSPLHLLIFRTLLTWPRHFRRDRQLLPLKHI 193 +I+ ++ L SPLHL RT+ ++ RH + ++ L+ I Sbjct: 300 LIYQKDFHLISPLHLTTLRTVPSFQRHIEQRLEIGDLESI 339 >SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 661 Score = 27.5 bits (58), Expect = 5.2 Identities = 19/46 (41%), Positives = 22/46 (47%), Gaps = 3/46 (6%) Frame = -3 Query: 252 LLTW--PRHFRRDRQLLPLKHIWRPL-TVCRHNLASSF*ISSIKNK 124 LLTW F R+R L H+ L T C H LAS+ I NK Sbjct: 354 LLTWLLDNSFFRNRHRLLAIHLATHLHTTCTHKLASTLIFKLINNK 399 >SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 557 Score = 27.1 bits (57), Expect = 6.9 Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 8/67 (11%) Frame = +2 Query: 446 SL*RTHSTPAGRIRSREVRRLFNYLWLLEIEMRRDLRILYKI--------IFFPQGNK*L 601 SL T +P +R V +++ L + E + I YK+ + FP+GNK Sbjct: 256 SLFWTPESPRYLLRHNHVEKVYKILSRIHPEAK-PAEIAYKVSLIQEGVKVDFPEGNKFQ 314 Query: 602 HFVYSMK 622 HF +S+K Sbjct: 315 HFFHSLK 321 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 27.1 bits (57), Expect = 6.9 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = -2 Query: 325 PLVRSDSFSKPATIFATSSFDISNPSDLAQTFPS*PAIAAFETH 194 PLV ++S S P++ +SNPS + PS P + E H Sbjct: 175 PLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSPLGSEDECH 218 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,561,973 Number of Sequences: 5004 Number of extensions: 80985 Number of successful extensions: 220 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 212 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 219 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 858281342 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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