Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_D04_e604_08.seq
(1529 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 102 5e-22
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 99 7e-21
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 96 5e-20
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 96 5e-20
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 95 1e-19
At1g27570.1 68414.m03360 phosphatidylinositol 3- and 4-kinase fa... 29 8.1
>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
thaliana]
Length = 134
Score = 102 bits (245), Expect = 5e-22
Identities = 56/133 (42%), Positives = 76/133 (57%), Gaps = 8/133 (6%)
Frame = +3
Query: 150 MSWQDYVDKQLMAS------RCVSKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 308
MSWQ YVD+ LM ++ AAI GHDG+VWA+S F + E+ I+ F+
Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEP 60
Query: 309 SLLTSGGVTIAGTRYIYLSGTER-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 485
L G+ +AG +Y+ + G +IR K G G+ KT Q++V LYEEP+ P Q
Sbjct: 61 GHLAPTGMFLAGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNM 120
Query: 486 VVEKLGDYLITCG 524
VVE+LGDYLI G
Sbjct: 121 VVERLGDYLIEQG 133
>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
thaliana]
Length = 134
Score = 99.1 bits (236), Expect = 7e-21
Identities = 54/133 (40%), Positives = 75/133 (56%), Gaps = 8/133 (6%)
Frame = +3
Query: 150 MSWQDYVDKQLMAS------RCVSKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 308
MSWQ YVD+ LM ++ AAI GHDG+VWA+S F + E + I+ F+
Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEP 60
Query: 309 SLLTSGGVTIAGTRYIYLSGTE-RIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 485
L G+ +AG +Y+ + G +IR K G G+ KT Q+ V +YEEP+ P Q
Sbjct: 61 GHLAPTGLFMAGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNM 120
Query: 486 VVEKLGDYLITCG 524
VVE+LGDYL+ G
Sbjct: 121 VVERLGDYLLEQG 133
>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
Length = 168
Score = 96.3 bits (229), Expect = 5e-20
Identities = 53/130 (40%), Positives = 72/130 (55%), Gaps = 5/130 (3%)
Frame = +3
Query: 150 MSWQDYVDKQLM---ASRCVSKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 317
MSWQ YVD LM A ++ AAI G DG+VWA+S F ++ +E+ I F L
Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTL 97
Query: 318 TSGGVTIAGTRYIYLSGTER-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 494
G+ + G +Y+ + G +IR K G GV KT A+V +Y+EP+ P Q VVE
Sbjct: 98 APTGLFLGGNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVE 157
Query: 495 KLGDYLITCG 524
LG+YLI G
Sbjct: 158 NLGEYLIESG 167
>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
thaliana]; identical to cDNA profilin (PRF2) GI:9965570
Length = 131
Score = 96.3 bits (229), Expect = 5e-20
Identities = 55/130 (42%), Positives = 73/130 (56%), Gaps = 5/130 (3%)
Frame = +3
Query: 150 MSWQDYVDKQLMAS---RCVSKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 317
MSWQ YVD LM ++ AAI G DG+VWA+S F ++ E+A I FE L
Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHL 60
Query: 318 TSGGVTIAGTRYIYLSGTE-RIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 494
G+ + G +Y+ + G +IR K G GV KT QA+V +Y+EP+ Q VVE
Sbjct: 61 APTGLFLGGEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVE 120
Query: 495 KLGDYLITCG 524
+LGDYLI G
Sbjct: 121 RLGDYLIESG 130
>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
Ara t 8 identical to profilin 1 (Allergen Ara t 8)
SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
Length = 131
Score = 95.1 bits (226), Expect = 1e-19
Identities = 53/127 (41%), Positives = 71/127 (55%), Gaps = 5/127 (3%)
Frame = +3
Query: 150 MSWQDYVDKQLMAS---RCVSKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 317
MSWQ YVD LM ++ AAI G DG+VWA+S F ++ E+ I FE L
Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60
Query: 318 TSGGVTIAGTRYIYLSGTE-RIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 494
G+ + G +Y+ + G + +IR K G GV KT QA+V Y+EP+ Q VVE
Sbjct: 61 APTGLFLGGEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVE 120
Query: 495 KLGDYLI 515
+LGDYLI
Sbjct: 121 RLGDYLI 127
>At1g27570.1 68414.m03360 phosphatidylinositol 3- and 4-kinase
family protein contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 649
Score = 29.1 bits (62), Expect = 8.1
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = -3
Query: 840 YTWSKKAXQNLKIQI*TVRYRXNYFVEAVSVQKESRIRKVIVTTH 706
Y W + NL + +V+ N FVE+V + E R+R+ H
Sbjct: 602 YKWEEDLDYNLHCIVASVQREDNNFVESVGTRIEQRLREFHENLH 646
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,150,905
Number of Sequences: 28952
Number of extensions: 424713
Number of successful extensions: 936
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 906
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 926
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4096401600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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