Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_C07_e627_05.seq
(1563 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 330 4e-91
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 171 2e-43
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 160 4e-40
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 122 2e-28
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 94 5e-20
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 84 4e-17
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 75 4e-14
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 9e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 42 2e-04
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 39 0.002
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 1.00
SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces... 27 7.0
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 27 9.3
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 330 bits (810), Expect = 4e-91
Identities = 155/176 (88%), Positives = 164/176 (93%)
Frame = +2
Query: 299 RXLTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITI 478
+ L ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA SSSLEKSYELPDGQVITI
Sbjct: 191 KILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITI 250
Query: 479 GNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIA 658
GNERFR PEALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIA
Sbjct: 251 GNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIA 310
Query: 659 DRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 826
DRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDESG
Sbjct: 311 DRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 366
Score = 133 bits (321), Expect = 7e-32
Identities = 62/66 (93%), Positives = 64/66 (96%)
Frame = +1
Query: 103 TFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 282
TFN PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRDL
Sbjct: 126 TFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDL 185
Query: 283 TDYLMK 300
TDYLMK
Sbjct: 186 TDYLMK 191
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 171 bits (416), Expect = 2e-43
Identities = 78/170 (45%), Positives = 112/170 (65%)
Frame = +2
Query: 305 LTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGN 484
L + G+ ++AE+EIVR+IKEK CYVA DF E+ + +Y+LPD Q IT+G
Sbjct: 198 LRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGT 257
Query: 485 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 664
E F PE LF P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +R
Sbjct: 258 ECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGER 317
Query: 665 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEY 814
E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY
Sbjct: 318 FISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY 367
Score = 89.0 bits (211), Expect = 2e-18
Identities = 41/82 (50%), Positives = 60/82 (73%), Gaps = 3/82 (3%)
Frame = +1
Query: 58 PGLQXIRHEXXLCSX---TFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYE 228
P L IR++ + T N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+
Sbjct: 113 PPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYD 172
Query: 229 GYALPHAILRLDLAGRDLTDYL 294
G+++P AI + ++ GRD+TDYL
Sbjct: 173 GFSIPSAIQQEEIGGRDVTDYL 194
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 160 bits (389), Expect = 4e-40
Identities = 81/186 (43%), Positives = 121/186 (65%), Gaps = 12/186 (6%)
Frame = +2
Query: 305 LTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGN 484
L +GY+F TA+ E VR++KEKLCYV+ D E + + ++ L ++Y LPDG+VI +G+
Sbjct: 193 LLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGS 252
Query: 485 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 664
ER+ CPE LFQP +G E G+ E +++I DVDIRK LY VLSGG++MY G+ R
Sbjct: 253 ERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSR 312
Query: 665 MQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQ 808
++KEI L P+ + K+KI P R+++V+IGG++LA ++ MW+SK
Sbjct: 313 LEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKA 372
Query: 809 EYDESG 826
E++E G
Sbjct: 373 EWEEYG 378
Score = 86.2 bits (204), Expect = 1e-17
Identities = 41/83 (49%), Positives = 55/83 (66%), Gaps = 3/83 (3%)
Frame = +1
Query: 58 PGLQXIRHEXXLCSXTFNT---PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYE 228
P + + + +C F +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE
Sbjct: 108 PPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYE 167
Query: 229 GYALPHAILRLDLAGRDLTDYLM 297
L H + RLD+AGRD T YL+
Sbjct: 168 SVVLNHLVGRLDVAGRDATRYLI 190
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 122 bits (293), Expect = 2e-28
Identities = 70/179 (39%), Positives = 99/179 (55%), Gaps = 11/179 (6%)
Frame = +2
Query: 323 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCP 502
S+ +R ++ + KE + V LD + A A+S + K +E PDG G ERFR
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV-LDTPFDEAKASSRNP--KPFEFPDGVTHKFGQERFRIS 299
Query: 503 EALFQPSFLGMEAC---------GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGI 655
E LF PSF + G+HE Y SI+ CD ++R L N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359
Query: 656 ADRMQKEITALAP-STMKIKII-APPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 826
++R+Q E+ LA S + + +VW GGSILASL FQ +W+SKQEYDE G
Sbjct: 360 SERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 93.9 bits (223), Expect = 5e-20
Identities = 46/97 (47%), Positives = 65/97 (67%), Gaps = 3/97 (3%)
Frame = +2
Query: 545 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 724
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 725 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESG 826
ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHG 421
Score = 35.9 bits (79), Expect = 0.015
Identities = 19/60 (31%), Positives = 29/60 (48%)
Frame = +1
Query: 115 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 294
PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G + D L
Sbjct: 128 PATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNAINDIL 187
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 84.2 bits (199), Expect = 4e-17
Identities = 57/182 (31%), Positives = 84/182 (46%), Gaps = 20/182 (10%)
Frame = +2
Query: 341 EREIVRDIKEKLCYVALDFEQEMATAASS--SSLEKSYELPD------------------ 460
E IV +IKE +CYV+ +F+++M S LE Y LPD
Sbjct: 211 ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIE 270
Query: 461 GQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTT 640
QV+ + NERF PE LF PS + + GI E S+ +I+ L N V GG
Sbjct: 271 QQVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNC 330
Query: 641 MYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 820
+PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E
Sbjct: 331 KFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSE 389
Query: 821 SG 826
G
Sbjct: 390 HG 391
Score = 35.1 bits (77), Expect = 0.027
Identities = 14/39 (35%), Positives = 25/39 (64%)
Frame = +1
Query: 178 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 294
+V+DSG +H +P+ + A+ R+D+ G+ LT+YL
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYL 197
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 74.5 bits (175), Expect = 4e-14
Identities = 50/174 (28%), Positives = 80/174 (45%), Gaps = 19/174 (10%)
Frame = +2
Query: 362 IKEKLCYVALDFEQEMATAASSSS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 529
IKE+ CYV D +E + L+ + E G TI G ERF PE F P
Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299
Query: 530 GME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST-- 700
+ + E N + +D+RK LY N VLSGG+T++ +R+Q+++ +
Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIH 359
Query: 701 ------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 826
+ + +I+ ++ +VW GGS+LA F +K +Y+E G
Sbjct: 360 RSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYG 413
Score = 64.1 bits (149), Expect = 5e-11
Identities = 32/72 (44%), Positives = 48/72 (66%), Gaps = 8/72 (11%)
Frame = +1
Query: 103 TFNTPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAILR 258
+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EGY + +I
Sbjct: 149 SFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKT 208
Query: 259 LDLAGRDLTDYL 294
+ LAGRD+T ++
Sbjct: 209 MPLAGRDVTYFV 220
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 66.5 bits (155), Expect = 9e-12
Identities = 30/114 (26%), Positives = 60/114 (52%)
Frame = +2
Query: 485 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 664
E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 665 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 826
+++E+T++ P I + W G S + F+ ++++EY E G
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKG 707
Score = 40.7 bits (91), Expect = 5e-04
Identities = 19/67 (28%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +1
Query: 106 FNTPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 279
+N P++ I + S Y + + ++GIVL+ G+ SH +P+ G + R+ G
Sbjct: 142 YNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQ 201
Query: 280 LTDYLMK 300
+ YL+K
Sbjct: 202 SSSYLLK 208
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 42.3 bits (95), Expect = 2e-04
Identities = 19/79 (24%), Positives = 39/79 (49%), Gaps = 3/79 (3%)
Frame = +2
Query: 599 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 769
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 770 SLSTFQQMWISKQEYDESG 826
+ ++WI ++ G
Sbjct: 587 RIRIVSELWIKNSDWKMLG 605
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 38.7 bits (86), Expect = 0.002
Identities = 20/60 (33%), Positives = 32/60 (53%)
Frame = +1
Query: 115 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 294
P +A + ++ LYA G G+V+D G + PI +G + A +L L GR +T +L
Sbjct: 176 PGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYMTQHL 235
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.9 bits (64), Expect = 1.00
Identities = 22/63 (34%), Positives = 27/63 (42%), Gaps = 3/63 (4%)
Frame = +2
Query: 104 PSTHPPCT*LSRPCSHC-TRPV--VPPVSCWIPAMVYPTQYPSTRVTPYRTPSCVWT*PV 274
P HPP LS+P S P+ VPPV + + P PS P PS P+
Sbjct: 987 PKDHPPSAPLSKPVSTSPAAPLARVPPVP-KLSSKAPPVPLPSADAPPIPVPSTAPPVPI 1045
Query: 275 ATS 283
TS
Sbjct: 1046 PTS 1048
>SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 419
Score = 27.1 bits (57), Expect = 7.0
Identities = 19/59 (32%), Positives = 25/59 (42%)
Frame = -3
Query: 664 TVGNTRVHGGTTRQYCVGVQVFTDVNVTLHDGVVCRLVDATSFHTQERGLEESLWATEP 488
T+G+ +GG GV V T VTLH G C + D T L+ + W P
Sbjct: 227 TLGDDWPNGGEI-DIVEGVNVGTSNQVTLHTGDGCEMEDIKRVMT-GTALQTNCWVDAP 283
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 26.6 bits (56), Expect = 9.3
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -3
Query: 697 GWSEGCDFLLHTVGNTRVHGGT 632
GW+ D +LH G V GGT
Sbjct: 240 GWTTPKDLILHLAGKLTVRGGT 261
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,671,635
Number of Sequences: 5004
Number of extensions: 87662
Number of successful extensions: 250
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 880085256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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