BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_B08_e634_04.seq (1525 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 83 7e-17 SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 79 2e-15 SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 76 1e-14 SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 64 6e-11 SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 55 3e-08 SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 48 3e-06 SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual 30 0.73 SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 29 1.3 SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 28 3.0 SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccha... 28 3.9 SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosacch... 28 3.9 SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosacchar... 28 3.9 SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccha... 28 3.9 SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 27 6.8 SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces pomb... 27 6.8 SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 27 9.0 SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosa... 27 9.0 >SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism |Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 83.4 bits (197), Expect = 7e-17 Identities = 59/161 (36%), Positives = 84/161 (52%), Gaps = 10/161 (6%) Frame = +3 Query: 246 FTLNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLL 425 FTL NG ++P+IGL T++ G T V ALKAGYR DTA +YGNE + ++ Sbjct: 15 FTLPNGDKIPSIGLGTWRSGKDETK-NAVCAALKAGYRHIDTAHIYGNEKEIGEGIRES- 72 Query: 426 PKYKLKRGDIFIISKLLPCNYGGGAVTERAFHNTLSNLGLDYIDLYLVHNP-----GIYK 590 + R DI++ SKL CN + A TL +L L+YID YL+H P G + Sbjct: 73 ---GVPRTDIWVTSKLW-CNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEE 128 Query: 591 LDFENRNSSRIR-----KQTWATLVDLYHDHKIRALGVSNY 698 L N I ++TW + +L K+R +G+SN+ Sbjct: 129 LP-RNEKGELIYEDVPIEETWQAMEELLETGKVRYIGISNF 168 >SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 78.6 bits (185), Expect = 2e-15 Identities = 53/150 (35%), Positives = 74/150 (49%) Frame = +3 Query: 249 TLNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLP 428 TL NG+ +P IG F + + V AL +GYR DTAA+YGNE A Sbjct: 12 TLTNGMVIPRIGFGAFML-KYNECYGLVTQALDSGYRHIDTAAVYGNEDICGKAIVDWCE 70 Query: 429 KYKLKRGDIFIISKLLPCNYGGGAVTERAFHNTLSNLGLDYIDLYLVHNPGIYKLDFENR 608 K +KR DIF+ SKL C+ T A ++L +LG YIDL+L+ +P Sbjct: 71 KNNVKRTDIFLTSKLANCS--DYYSTRAAIRSSLHHLG-TYIDLFLIQSPA--------- 118 Query: 609 NSSRIRKQTWATLVDLYHDHKIRALGVSNY 698 + R +W + + IR++GVSNY Sbjct: 119 GGKKSRIASWKAMEEFVDSGDIRSVGVSNY 148 >SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 76.2 bits (179), Expect = 1e-14 Identities = 53/160 (33%), Positives = 83/160 (51%), Gaps = 9/160 (5%) Frame = +3 Query: 246 FTLNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLL 425 FTL +G ++P +GL T++ T V AL+ GYR D AA+YGNE + K+ Sbjct: 16 FTLADGSKIPGLGLGTWRSEPNQTK-NAVKTALQYGYRHIDAAAIYGNEDEVGDGIKES- 73 Query: 426 PKYKLKRGDIFIISKLLPCNYGGGAVTERAFHNTLSNLGLDYIDLYLVHNPGIYKLDFE- 602 + R DI++ SKL CN +A TL +L LDY+D YL+H P +K + Sbjct: 74 ---GVPRKDIWVTSKLW-CNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDK 129 Query: 603 ---NRNSSRIR-----KQTWATLVDLYHDHKIRALGVSNY 698 +++ + I ++TW + L K+R +G+SN+ Sbjct: 130 FPKDKDGNLIYEKNPIEETWKAMEKLLETGKVRHIGLSNF 169 >SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 275 Score = 63.7 bits (148), Expect = 6e-11 Identities = 47/149 (31%), Positives = 72/149 (48%) Frame = +3 Query: 252 LNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLPK 431 LNNG++ P ++ + +V AL+ GYR D+A +Y NE A K + + Sbjct: 8 LNNGLKCPQFAYGSYMVNRT-KCFDSVYAALQCGYRHIDSAQMYHNEADCGRAILKFMEE 66 Query: 432 YKLKRGDIFIISKLLPCNYGGGAVTERAFHNTLSNLGLDYIDLYLVHNPGIYKLDFENRN 611 KR DI+ SKL + G T + ++ GL YIDL+L+H+P Sbjct: 67 TGTKREDIWFTSKL--NDLSGYKSTLSSIDASVKACGLGYIDLFLLHSP----------Y 114 Query: 612 SSRIRKQTWATLVDLYHDHKIRALGVSNY 698 RI ++W L + K+RA+GVSN+ Sbjct: 115 GDRI--ESWKALEKGVEEGKLRAIGVSNF 141 >SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reductase AKR3C2|Schizosaccharomyces pombe|chr 1|||Manual Length = 284 Score = 54.8 bits (126), Expect = 3e-08 Identities = 39/110 (35%), Positives = 61/110 (55%), Gaps = 5/110 (4%) Frame = +3 Query: 264 VEMPAIGLDT--FKM--GHFY-TVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLP 428 + +PA G+ T FK G T++ +V +AL AG+ D A +YGNE + A K+ Sbjct: 10 IPVPAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAEVYGNEEEVGVALKEA-- 67 Query: 429 KYKLKRGDIFIISKLLPCNYGGGAVTERAFHNTLSNLGLDYIDLYLVHNP 578 + R +FI SK++ + + E A + +L LG DY+DLYL+H+P Sbjct: 68 --NVPRSKLFITSKVM---HNVDNIPE-ALNESLRKLGTDYLDLYLLHSP 111 >SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces pombe|chr 2|||Manual Length = 276 Score = 48.0 bits (109), Expect = 3e-06 Identities = 39/128 (30%), Positives = 62/128 (48%), Gaps = 1/128 (0%) Frame = +3 Query: 324 QTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLPK-YKLKRGDIFIISKLLPCNYGGGA 500 Q V +AL G R+ D+A Y NE A + + +KR DI +I+K+ P + G Sbjct: 28 QQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITKI-PDSLQGFE 86 Query: 501 VTERAFHNTLSNLGLDYIDLYLVHNPGIYKLDFENRNSSRIRKQTWATLVDLYHDHKIRA 680 T +A +L G +D+ L+H+P + R R ++W L+ + +I Sbjct: 87 RTWKAVEQSLRRTGRPKLDVVLIHSP-----KWPVR-----RIESWRALLQHQKEGRINK 136 Query: 681 LGVSNYFI 704 +GVSNY I Sbjct: 137 IGVSNYNI 144 >SPCC11E10.02c |gpi8||pig-K|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 30.3 bits (65), Expect = 0.73 Identities = 8/28 (28%), Positives = 19/28 (67%) Frame = -2 Query: 507 QSLHHHRNYMEVIYLL*ICPLSSIYTLV 424 + +H H+ Y E+++++ C +S+YT + Sbjct: 169 EQIHQHKRYNEILFMVDTCQANSLYTKI 196 >SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 344 Score = 29.5 bits (63), Expect = 1.3 Identities = 24/83 (28%), Positives = 34/83 (40%), Gaps = 7/83 (8%) Frame = +3 Query: 351 GYRMFDTAALYGN---EVYLNFAFKKL---LPKYKLKRGDIFIISKLLPCNYG-GGAVTE 509 G FDTA +Y N E + A K+L +Y + F LP G Sbjct: 57 GINTFDTAEIYSNGNSETVMGKAIKELGWDRSEYVITTKVFFGAGTKLPNTTGLSRKHII 116 Query: 510 RAFHNTLSNLGLDYIDLYLVHNP 578 + +L LGL Y+D+ + H P Sbjct: 117 EGLNASLKRLGLPYVDVIMAHRP 139 >SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizosaccharomyces pombe|chr 1|||Manual Length = 340 Score = 28.3 bits (60), Expect = 3.0 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +3 Query: 507 ERAFHNTLSNLGLDYIDLYLVH 572 E+A +L LG+D IDLY VH Sbjct: 105 EKALDLSLKRLGIDCIDLYYVH 126 >SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 2|||Manual Length = 344 Score = 27.9 bits (59), Expect = 3.9 Identities = 19/73 (26%), Positives = 32/73 (43%) Frame = +3 Query: 249 TLNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLP 428 TL +E + K FY+V + +D YR+F +Y L F + +P Sbjct: 144 TLQECIEEYVFSSECIKYRQFYSVYEIIDFLRTNFYRLF---VIY---CALGFGITRTVP 197 Query: 429 KYKLKRGDIFIIS 467 KY + +G +I+ Sbjct: 198 KYLMIKGISIVIA 210 >SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 2|||Manual Length = 344 Score = 27.9 bits (59), Expect = 3.9 Identities = 19/73 (26%), Positives = 32/73 (43%) Frame = +3 Query: 249 TLNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLP 428 TL +E + K FY+V + +D YR+F +Y L F + +P Sbjct: 144 TLQECIEEYVFSSECIKYRQFYSVYEIIDFLRTNFYRLF---VIY---CALGFGITRTVP 197 Query: 429 KYKLKRGDIFIIS 467 KY + +G +I+ Sbjct: 198 KYLMIKGISIVIA 210 >SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 316 Score = 27.9 bits (59), Expect = 3.9 Identities = 19/73 (26%), Positives = 32/73 (43%) Frame = +3 Query: 249 TLNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLP 428 TL +E + K FY+V + +D YR+F +Y L F + +P Sbjct: 116 TLQECIEEYVFSSECIKYRQFYSVYEIIDFLRTNFYRLF---VIY---CALGFGITRTVP 169 Query: 429 KYKLKRGDIFIIS 467 KY + +G +I+ Sbjct: 170 KYLMIKGISIVIA 182 >SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 27.9 bits (59), Expect = 3.9 Identities = 19/73 (26%), Positives = 32/73 (43%) Frame = +3 Query: 249 TLNNGVEMPAIGLDTFKMGHFYTVLQTVDDALKAGYRMFDTAALYGNEVYLNFAFKKLLP 428 TL +E + K FY+V + +D YR+F +Y L F + +P Sbjct: 144 TLQECIEEYVFSSECIKYRQFYSVYEIIDFLRTNFYRLF---VIY---CALGFGITRTVP 197 Query: 429 KYKLKRGDIFIIS 467 KY + +G +I+ Sbjct: 198 KYLMIKGISIVIA 210 >SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 27.1 bits (57), Expect = 6.8 Identities = 12/39 (30%), Positives = 21/39 (53%) Frame = +3 Query: 603 NRNSSRIRKQTWATLVDLYHDHKIRALGVSNYFIWGSYG 719 N + R+R LV+L+ + +R+L + YF G +G Sbjct: 709 NASMVRLRSTFNEELVELFENMAVRSLNRAEYFCTGDFG 747 >SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 27.1 bits (57), Expect = 6.8 Identities = 9/28 (32%), Positives = 19/28 (67%) Frame = -3 Query: 644 SSPSLFSNSGTVPIFKIQFINSRVMDQI 561 +SP F N+ T P+ + ++N++++D I Sbjct: 96 TSPGDFENASTSPVSDVTYVNTKLVDFI 123 >SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 26.6 bits (56), Expect = 9.0 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 636 WATLVDLYHDHKIRALGVSNYFIW 707 WAT VDLY + I L + + F+W Sbjct: 110 WATEVDLYGNPPILKLQLRDSFVW 133 >SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1215 Score = 26.6 bits (56), Expect = 9.0 Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 3/54 (5%) Frame = +3 Query: 516 FHNTLSNLGLDYID--LYLVHNPGIYKLDF-ENRNSSRIRKQTWATLVDLYHDH 668 FH+ + ++G DY D + LV L F +++N++ R QT+ T L+ H Sbjct: 239 FHSIIGDVGTDYYDDHISLVFRFRFSPLIFRKSKNATESRVQTFWTASHLWRRH 292 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,834,377 Number of Sequences: 5004 Number of extensions: 90902 Number of successful extensions: 232 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 210 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 223 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 854316994 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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