BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_B05_e610_03.seq
(1582 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 30 1.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 9.4
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 9.4
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 29.9 bits (64), Expect = 1.0
Identities = 18/53 (33%), Positives = 26/53 (49%)
Frame = -2
Query: 750 DFXPIGLKMS*XNKNLTRNFNKILTLTICHSPFRLRNCWEGRSVRASSLLRQL 592
DF + S NK L +N ++ LT+ IC S + E S + S LL+ L
Sbjct: 525 DFSILVQFYSIQNKMLVKNGDEALTIAICDSRCLIARGTESNSFKRSDLLKSL 577
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 26.6 bits (56), Expect = 9.4
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
Frame = +3
Query: 474 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 638
P P+ S ++ H + + +L N I+L ++PLSP A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231
Query: 639 SCA 647
S A
Sbjct: 232 SDA 234
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 26.6 bits (56), Expect = 9.4
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 581 PPFASWRNSEEARTDRPSQQLRSLN 655
PP AS RN E + PS+Q S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,377,450
Number of Sequences: 5004
Number of extensions: 71055
Number of successful extensions: 140
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 891978300
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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