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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030731E7_A07_e625_01.seq
         (1581 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g74470.1 68414.m08627 geranylgeranyl reductase identical to g...    38   0.024
At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R6...    33   0.52 
At4g32640.1 68417.m04646 sec23/sec24 transport protein-related         31   2.1  
At1g50970.1 68414.m05730 membrane trafficking VPS53 family prote...    31   2.1  
At5g47490.1 68418.m05864 expressed protein                             29   8.4  
At2g18470.1 68415.m02151 protein kinase family protein contains ...    29   8.4  

>At1g74470.1 68414.m08627 geranylgeranyl reductase identical to
           geranylgeranyl reductase GB:Y14044 [Arabidopsis
           thaliana] (involvement: chlorophyll, the tocopherol and
           the phylloquinone pathways Eur J Biochem 1998 Jan
           15;251(1-2):413-7)
          Length = 467

 Score = 37.5 bits (83), Expect = 0.024
 Identities = 18/42 (42%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
 Frame = +3

Query: 576 DKEDALKNLIQLLKKDD-SPVNYGYVFALCEHMGCGTWTVTH 698
           +K    ++L ++   DD SP  YG+VF  C+H+  GT TVTH
Sbjct: 242 EKMTYYEDLAEMYVGDDVSPDFYGWVFPKCDHVAVGTGTVTH 283


>At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966,
           gb|R65511, gb|T42324 and gb|T20569 come from this gene
          Length = 571

 Score = 33.1 bits (72), Expect = 0.52
 Identities = 25/96 (26%), Positives = 47/96 (48%)
 Frame = +3

Query: 213 AISNANILAGTIELKRLQSILEDSLKSKDISSLYYAVKGLKLLKAPIPDICEDIKTIKYD 392
           A   AN+     E K LQ   E++ K +D   L    + +  L+  I  IC+D+K +K  
Sbjct: 431 AEQEANMEKVVQESKLLQQEAEENSKLRDF--LMDRGQIVDTLQGEISVICQDVKLLKEK 488

Query: 393 VKNIEQIFQLTNAAALTDCVNFLKPEVLSTPAQVLD 500
            +N   + +  +++  + C + +K  VL  P++ L+
Sbjct: 489 FENRVPLTKSISSSFTSSCGSSMKSLVLENPSERLN 524


>At4g32640.1 68417.m04646 sec23/sec24 transport protein-related
          Length = 1069

 Score = 31.1 bits (67), Expect = 2.1
 Identities = 20/68 (29%), Positives = 22/68 (32%)
 Frame = +1

Query: 1069 GGPXPGPGXPXXXXGVPXXVAQADGPXPCXNXXXSXSGXPXPXPRGKXXNXXXXXXXGRH 1248
            G P  GP  P    G P  +A+  GP P        S  P   P G              
Sbjct: 76   GAPQRGPS-PMSRPGPPAGMARPGGPPPVSQPAGFQSNVPLNRPTGPPSRQPSFGSRPSM 134

Query: 1249 PXGRGAQP 1272
            P G  AQP
Sbjct: 135  PGGPVAQP 142


>At1g50970.1 68414.m05730 membrane trafficking VPS53 family protein
           contains Pfam domain PF04100: Vps53-like, N-terminal
          Length = 569

 Score = 31.1 bits (67), Expect = 2.1
 Identities = 30/123 (24%), Positives = 57/123 (46%), Gaps = 3/123 (2%)
 Frame = +3

Query: 258 RLQSILEDSL-KSKDISSLYYAVKG-LKLLKAPIPDICEDIKTIKYDVKNIEQIFQLTNA 431
           R +  L D++  ++++S     +K   +  +A + DIC DIK + +  KNI     +T  
Sbjct: 54  RAKENLNDAICAAEELSHKIQEIKSKAEQTEAMVQDICSDIKKLDFAKKNITT--AVTAL 111

Query: 432 AALTDCVNFLKPEVLSTPAQVLDKKDATLSEIYYSVYALKA-LGKGSVFDKEDALKNLIQ 608
           + LT  V+ ++   + T  +   +    L  I       KA +    + +  + LKN+ Q
Sbjct: 112 SRLTMLVSAVQQLQVMTSKRQYKEAATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQ 171

Query: 609 LLK 617
           +LK
Sbjct: 172 ILK 174


>At5g47490.1 68418.m05864 expressed protein
          Length = 1361

 Score = 29.1 bits (62), Expect = 8.4
 Identities = 13/35 (37%), Positives = 17/35 (48%)
 Frame = -1

Query: 801 VLISRRXTRK*LAGHLRNEXTWNQFHQRPAAHPPH 697
           V   R+ T   +AG + N  TWNQ  Q    +P H
Sbjct: 279 VAYQRQSTASAVAGTVENVSTWNQVSQVSNGYPEH 313


>At2g18470.1 68415.m02151 protein kinase family protein contains Pfam
            PF00069: Protein kinase domain
          Length = 633

 Score = 29.1 bits (62), Expect = 8.4
 Identities = 26/111 (23%), Positives = 31/111 (27%), Gaps = 6/111 (5%)
 Frame = +1

Query: 1060 TXGGGPXPGPGXPXXXXGVPXXVAQADGPXP----CXNXXXSXSGXPXPXPRGKXXNXXX 1227
            T    P P P  P    G        D   P      N   S +  P P  +G       
Sbjct: 26   TTSSPPAPSPPSPTPPQGDSSSSPPPDSTSPPAPQAPNPPNSSNNSPSPPSQGGGGERGN 85

Query: 1228 XXXXGRH--PXGRGAQPXXPAXYPXXXXTXRPXFPXXTRXPRRXXPPQFXG 1374
                G +  P  RG+ P  P+         R   P  T   R   PP   G
Sbjct: 86   GGNNGGNDTPPSRGSPPSPPSRSNGDNGGSRSSPPGDTGGSRSDNPPSSGG 136


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,285,971
Number of Sequences: 28952
Number of extensions: 428708
Number of successful extensions: 824
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 767
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 822
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4260257664
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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