BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_A07_e625_01.seq (1581 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74470.1 68414.m08627 geranylgeranyl reductase identical to g... 38 0.024 At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R6... 33 0.52 At4g32640.1 68417.m04646 sec23/sec24 transport protein-related 31 2.1 At1g50970.1 68414.m05730 membrane trafficking VPS53 family prote... 31 2.1 At5g47490.1 68418.m05864 expressed protein 29 8.4 At2g18470.1 68415.m02151 protein kinase family protein contains ... 29 8.4 >At1g74470.1 68414.m08627 geranylgeranyl reductase identical to geranylgeranyl reductase GB:Y14044 [Arabidopsis thaliana] (involvement: chlorophyll, the tocopherol and the phylloquinone pathways Eur J Biochem 1998 Jan 15;251(1-2):413-7) Length = 467 Score = 37.5 bits (83), Expect = 0.024 Identities = 18/42 (42%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = +3 Query: 576 DKEDALKNLIQLLKKDD-SPVNYGYVFALCEHMGCGTWTVTH 698 +K ++L ++ DD SP YG+VF C+H+ GT TVTH Sbjct: 242 EKMTYYEDLAEMYVGDDVSPDFYGWVFPKCDHVAVGTGTVTH 283 >At1g03290.1 68414.m00307 expressed protein ESTs gb|H36966, gb|R65511, gb|T42324 and gb|T20569 come from this gene Length = 571 Score = 33.1 bits (72), Expect = 0.52 Identities = 25/96 (26%), Positives = 47/96 (48%) Frame = +3 Query: 213 AISNANILAGTIELKRLQSILEDSLKSKDISSLYYAVKGLKLLKAPIPDICEDIKTIKYD 392 A AN+ E K LQ E++ K +D L + + L+ I IC+D+K +K Sbjct: 431 AEQEANMEKVVQESKLLQQEAEENSKLRDF--LMDRGQIVDTLQGEISVICQDVKLLKEK 488 Query: 393 VKNIEQIFQLTNAAALTDCVNFLKPEVLSTPAQVLD 500 +N + + +++ + C + +K VL P++ L+ Sbjct: 489 FENRVPLTKSISSSFTSSCGSSMKSLVLENPSERLN 524 >At4g32640.1 68417.m04646 sec23/sec24 transport protein-related Length = 1069 Score = 31.1 bits (67), Expect = 2.1 Identities = 20/68 (29%), Positives = 22/68 (32%) Frame = +1 Query: 1069 GGPXPGPGXPXXXXGVPXXVAQADGPXPCXNXXXSXSGXPXPXPRGKXXNXXXXXXXGRH 1248 G P GP P G P +A+ GP P S P P G Sbjct: 76 GAPQRGPS-PMSRPGPPAGMARPGGPPPVSQPAGFQSNVPLNRPTGPPSRQPSFGSRPSM 134 Query: 1249 PXGRGAQP 1272 P G AQP Sbjct: 135 PGGPVAQP 142 >At1g50970.1 68414.m05730 membrane trafficking VPS53 family protein contains Pfam domain PF04100: Vps53-like, N-terminal Length = 569 Score = 31.1 bits (67), Expect = 2.1 Identities = 30/123 (24%), Positives = 57/123 (46%), Gaps = 3/123 (2%) Frame = +3 Query: 258 RLQSILEDSL-KSKDISSLYYAVKG-LKLLKAPIPDICEDIKTIKYDVKNIEQIFQLTNA 431 R + L D++ ++++S +K + +A + DIC DIK + + KNI +T Sbjct: 54 RAKENLNDAICAAEELSHKIQEIKSKAEQTEAMVQDICSDIKKLDFAKKNITT--AVTAL 111 Query: 432 AALTDCVNFLKPEVLSTPAQVLDKKDATLSEIYYSVYALKA-LGKGSVFDKEDALKNLIQ 608 + LT V+ ++ + T + + L I KA + + + + LKN+ Q Sbjct: 112 SRLTMLVSAVQQLQVMTSKRQYKEAATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQ 171 Query: 609 LLK 617 +LK Sbjct: 172 ILK 174 >At5g47490.1 68418.m05864 expressed protein Length = 1361 Score = 29.1 bits (62), Expect = 8.4 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = -1 Query: 801 VLISRRXTRK*LAGHLRNEXTWNQFHQRPAAHPPH 697 V R+ T +AG + N TWNQ Q +P H Sbjct: 279 VAYQRQSTASAVAGTVENVSTWNQVSQVSNGYPEH 313 >At2g18470.1 68415.m02151 protein kinase family protein contains Pfam PF00069: Protein kinase domain Length = 633 Score = 29.1 bits (62), Expect = 8.4 Identities = 26/111 (23%), Positives = 31/111 (27%), Gaps = 6/111 (5%) Frame = +1 Query: 1060 TXGGGPXPGPGXPXXXXGVPXXVAQADGPXP----CXNXXXSXSGXPXPXPRGKXXNXXX 1227 T P P P P G D P N S + P P +G Sbjct: 26 TTSSPPAPSPPSPTPPQGDSSSSPPPDSTSPPAPQAPNPPNSSNNSPSPPSQGGGGERGN 85 Query: 1228 XXXXGRH--PXGRGAQPXXPAXYPXXXXTXRPXFPXXTRXPRRXXPPQFXG 1374 G + P RG+ P P+ R P T R PP G Sbjct: 86 GGNNGGNDTPPSRGSPPSPPSRSNGDNGGSRSSPPGDTGGSRSDNPPSSGG 136 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 24,285,971 Number of Sequences: 28952 Number of extensions: 428708 Number of successful extensions: 824 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 767 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 822 length of database: 12,070,560 effective HSP length: 84 effective length of database: 9,638,592 effective search space used: 4260257664 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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