BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030731E7_A02_e585_02.seq (1463 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 31 0.53 SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom... 30 0.70 SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|... 29 1.6 SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 2.8 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 28 2.8 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 28 3.7 SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 27 4.9 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 27 6.5 SPBC16A3.18 |cip1||RNA-binding protein Cip1|Schizosaccharomyces ... 27 8.6 >SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr 1|||Manual Length = 1778 Score = 30.7 bits (66), Expect = 0.53 Identities = 17/39 (43%), Positives = 24/39 (61%) Frame = -2 Query: 514 SVGFGSADLGSGVFSSALTLSSGFGFVSSGCLVSASAGA 398 S GFGS G G+F S+ T ++ F +SG VS++A A Sbjct: 137 STGFGSQGTGGGLFGSSTTPATTNAFGTSG-FVSSNANA 174 >SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 963 Score = 30.3 bits (65), Expect = 0.70 Identities = 22/89 (24%), Positives = 34/89 (38%), Gaps = 1/89 (1%) Frame = +3 Query: 471 EKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKVNEVPTEVSEKSASP 650 +K P PK+ + P PS SKDT V + K+ +P Sbjct: 127 KKKPIPKTKSKPTSHAPVSDNVSSTFRNATRKSKKPSASKDTSRGVRK------SKAGAP 180 Query: 651 DKPIDVGSATKPDAPV-THDGPSEHVTSK 734 P V + + + P T D PS +++K Sbjct: 181 SDPSSVHAPSSLEKPAGTGDLPSSEISTK 209 >SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 29.1 bits (62), Expect = 1.6 Identities = 17/53 (32%), Positives = 24/53 (45%) Frame = +3 Query: 594 TPAKVNEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGPSEHVTSKPRKKVD 752 +P ++NE P P KPID GS +P P + +S P K+D Sbjct: 92 SPLRLNEAPRRRGRPRKYPPKPIDEGS--EPIIKRKRGRPPKIKSSSPSTKLD 142 >SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 28.3 bits (60), Expect = 2.8 Identities = 18/55 (32%), Positives = 24/55 (43%) Frame = -1 Query: 422 FGLSFCWCFFTFGLRYFFFSFWSRIGFLFLFRNGMLCFSGFCVSYRSRLSNQFLF 258 F SF + FF + +FS + + FLF F + F F S LS LF Sbjct: 129 FSFSFSFLFFLSQIFIVYFSSFPILHFLFFFFLCVCVFLSFLFSLSHLLSLAILF 183 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 28.3 bits (60), Expect = 2.8 Identities = 26/110 (23%), Positives = 44/110 (40%), Gaps = 2/110 (1%) Frame = +3 Query: 435 TKPKPDDKVKADEKTPEPKSAEPKPTD-VPAXXXXXXXXXXXXXXXXVPSKSKDTPA-KV 608 TK ++ A+++ +P EP T A S +P K Sbjct: 29 TKASENNTATAEKQAVKPSGVEPTNTSRANAQKKTESTGKITSEADTEKYNSSKSPVNKE 88 Query: 609 NEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGPSEHVTSKPRKKVDSS 758 V + SEKS++ +KP + +KP A + +E +S+ +K SS Sbjct: 89 GSVEKKSSEKSSTNNKPWRGDNTSKPSA----NSSAERTSSQHQKPETSS 134 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 27.9 bits (59), Expect = 3.7 Identities = 14/43 (32%), Positives = 22/43 (51%) Frame = +1 Query: 430 KRQNQNQMIKSKQMKRHLNLNLQNQSLPTFLLNLNQKRSPRIV 558 K N + K+ +RH ++ PT + NLN R+P+IV Sbjct: 470 KESLSNDQLSVKEKRRH------HKKAPTLIQNLNSPRTPKIV 506 >SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schizosaccharomyces pombe|chr 2|||Manual Length = 965 Score = 27.5 bits (58), Expect = 4.9 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 435 TKPKPDDKVKADEKTPEPKSAEPKPTD 515 +K DD++K D+KT E K A P D Sbjct: 835 SKGSNDDEMKIDKKTTEEKEATPMEMD 861 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 27.1 bits (57), Expect = 6.5 Identities = 17/37 (45%), Positives = 24/37 (64%) Frame = -2 Query: 517 TSVGFGSADLGSGVFSSALTLSSGFGFVSSGCLVSAS 407 +S GF S+ +G SS+ +L+S G VSS LVS+S Sbjct: 3517 SSGGFSSSAFSTG--SSSFSLTSENGSVSSSSLVSSS 3551 >SPBC16A3.18 |cip1||RNA-binding protein Cip1|Schizosaccharomyces pombe|chr 2|||Manual Length = 490 Score = 26.6 bits (56), Expect = 8.6 Identities = 15/55 (27%), Positives = 27/55 (49%) Frame = +3 Query: 153 ITSNPTSQDLNFTMKVLLFCMAFAAVTIAKPVADEKQELVAQPTSVADTKTTETQ 317 IT TS+D N + +FC+ F A+P + + +V P +T +++Q Sbjct: 345 ITFETTSKDGNLLNAIRIFCLYFDLDYYARPNGEVLKLVVTHPNK-KNTSVSQSQ 398 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,165,294 Number of Sequences: 5004 Number of extensions: 67992 Number of successful extensions: 246 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 220 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 244 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 814673514 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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