Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030731E7_A02_e585_02.seq
(1463 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 33 0.62
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 32 0.83
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 32 1.1
At1g49490.1 68414.m05547 leucine-rich repeat family protein / ex... 32 1.1
At3g61050.1 68416.m06832 calcium-dependent lipid-binding protein... 31 1.9
At4g33970.1 68417.m04820 leucine-rich repeat family protein / ex... 31 2.5
At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /... 31 2.5
At5g56330.1 68418.m07031 carbonic anhydrase family protein conta... 30 3.3
At1g26150.1 68414.m03192 protein kinase family protein similar t... 30 3.3
At5g67470.1 68418.m08507 formin homology 2 domain-containing pro... 30 4.4
At1g61080.1 68414.m06877 proline-rich family protein 30 4.4
At1g01730.1 68414.m00092 expressed protein 30 4.4
At5g66830.1 68418.m08426 F-box family protein contains F-box dom... 29 5.8
At3g50650.1 68416.m05540 scarecrow-like transcription factor 7 (... 29 5.8
At3g50180.1 68416.m05486 hypothetical protein 29 5.8
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 29 5.8
At1g59910.1 68414.m06749 formin homology 2 domain-containing pro... 29 5.8
At5g38560.1 68418.m04662 protein kinase family protein contains ... 29 7.7
At5g27120.1 68418.m03237 SAR DNA-binding protein, putative stron... 29 7.7
>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 956
Score = 32.7 bits (71), Expect = 0.62
Identities = 24/107 (22%), Positives = 41/107 (38%), Gaps = 3/107 (2%)
Frame = +3
Query: 429 EETKPKPDDKVKADEKTPEPK--SAEPKPTDVPAXXXXXXXXXXXXXXXXVP-SKSKDTP 599
E +KPKP++ K + +P+P+ S EP P P S +++P
Sbjct: 431 EPSKPKPEESPKPQQPSPKPETPSHEPSNPKEPKPESPKQESPKTEQPKPKPESPKQESP 490
Query: 600 AKVNEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGPSEHVTSKPR 740
+ P + K SP + KP+ + P + KP+
Sbjct: 491 KQEAPKPEQPKPKPESPKQESSKQEPPKPEESPKPEPPKPEESPKPQ 537
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 32.3 bits (70), Expect = 0.83
Identities = 13/26 (50%), Positives = 13/26 (50%)
Frame = -2
Query: 1321 PAQTPXXXPPPRAXKXPPPXPP*IGP 1244
P TP PPP PPP PP I P
Sbjct: 593 PPPTPVSSPPPTPVYSPPPPPPCIEP 618
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 31.9 bits (69), Expect = 1.1
Identities = 22/87 (25%), Positives = 30/87 (34%)
Frame = +3
Query: 441 PKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKVNEVP 620
P P V + TP P + P P+ VP+ P+ P+ P
Sbjct: 430 PSPPITVPSPPTTPSPGGSPPSPSIVPSPPSTTPSPGSPPTSPTTPTPGGSPPSSPT-TP 488
Query: 621 TEVSEKSASPDKPIDVGSATKPDAPVT 701
T +SP P GS P +P T
Sbjct: 489 TPGGSPPSSPTTPTPGGS--PPSSPTT 513
Score = 30.7 bits (66), Expect = 2.5
Identities = 24/103 (23%), Positives = 30/103 (29%), Gaps = 4/103 (3%)
Frame = +3
Query: 435 TKPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKVNE 614
T P P + P P S P P P P+ P+
Sbjct: 441 TTPSPGGSPPSPSIVPSPPSTTPSPGSPPTSPTTPTPGGSPPSSPTTPTPGGSPPSSPT- 499
Query: 615 VPTEVSEKSASPDKPIDVGS----ATKPDAPVTHDGPSEHVTS 731
PT +SP P GS + P P+T P TS
Sbjct: 500 TPTPGGSPPSSPTTPSPGGSPPSPSISPSPPITVPSPPSTPTS 542
>At1g49490.1 68414.m05547 leucine-rich repeat family protein /
extensin family protein contains similarity to disease
resistance protein GI:3894383 from [Lycopersicon
esculentum]; contains leucine-rich repeats,
Pfam:PF00560; contains proline rich extensin domains,
INTERPRO:IPR002965
Length = 847
Score = 31.9 bits (69), Expect = 1.1
Identities = 23/89 (25%), Positives = 35/89 (39%), Gaps = 5/89 (5%)
Frame = +3
Query: 492 SAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKVNEVPTEVSEKSASPDK---PI 662
S+EP P+ P ++ T ++ ++VPT SE + SP + PI
Sbjct: 696 SSEPTQVPTPSSSESYQAPNLSPVQAPTPVQAPTTSSETSQVPTPSSESNQSPSQAPTPI 755
Query: 663 --DVGSATKPDAPVTHDGPSEHVTSKPRK 743
V + T PV PS S P +
Sbjct: 756 LEPVHAPTPNSKPVQSPTPSSEPVSSPEQ 784
Score = 30.3 bits (65), Expect = 3.3
Identities = 21/95 (22%), Positives = 35/95 (36%), Gaps = 2/95 (2%)
Frame = +3
Query: 435 TKPKPDDKVKADEKTPEPKSAEPKPTD--VPAXXXXXXXXXXXXXXXXVPSKSKDTPAKV 608
+ P P+ ++ K +P+ PKP+D P S + P
Sbjct: 392 SSPSPNPPRTSEPKPSKPEPVMPKPSDSSKPETPKTPEQPSPKPQPPKHESPKPEEPENK 451
Query: 609 NEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGP 713
+E+P + P KP D + KP+ P + P
Sbjct: 452 HELPKQKESPKPQPSKPED---SPKPEQPKPEESP 483
>At3g61050.1 68416.m06832 calcium-dependent lipid-binding protein,
putative strong similarity to CLB1 [Lycopersicon
esculentum] GI:2789434; contains Pfam profile PF00168:
C2 domain
Length = 510
Score = 31.1 bits (67), Expect = 1.9
Identities = 18/42 (42%), Positives = 23/42 (54%)
Frame = -2
Query: 517 TSVGFGSADLGSGVFSSALTLSSGFGFVSSGCLVSASAGAFL 392
T +G G +GSGV S + SGFG V SG + AG F+
Sbjct: 442 TGIGTGVGLVGSGVSSGVGMVGSGFGAVGSGL---SKAGRFM 480
>At4g33970.1 68417.m04820 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 699
Score = 30.7 bits (66), Expect = 2.5
Identities = 33/117 (28%), Positives = 40/117 (34%), Gaps = 11/117 (9%)
Frame = +3
Query: 438 KPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPA-KVNE 614
KP P + TP P + KP+ VP PS TP K +
Sbjct: 427 KPTPVPTTPVHKPTPVPTTPVQKPSPVPTTPVQKPSPVPTTPVHE-PSPVLATPVDKPSP 485
Query: 615 VPTEVSEK------SASPDKPIDVGSATK----PDAPVTHDGPSEHVTSKPRKKVDS 755
VP+ +K S PD P D TK P APV P + P V S
Sbjct: 486 VPSRPVQKPQPPKESPQPDDPYDQSPVTKRRSPPPAPVNSPPPPVYSPPPPPPPVHS 542
>At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
SEC14-like protein 2 (Alpha-tocopherol associated
protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos
taurus}; similar to GI:807956 from [Saccharomyces
cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 573
Score = 30.7 bits (66), Expect = 2.5
Identities = 26/75 (34%), Positives = 33/75 (44%)
Frame = +3
Query: 429 EETKPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKV 608
EETK + + K +E T E K E KP VPA V +KS++ P +
Sbjct: 132 EETKEEEKTEEKKEETTTEVKVEEEKPA-VPA-----AEEEKSSEAAPVETKSEEKPEEK 185
Query: 609 NEVPTEVSEKSASPD 653
EV TE SA D
Sbjct: 186 AEVTTE-KASSAEED 199
>At5g56330.1 68418.m07031 carbonic anhydrase family protein contains
proline-rich extensin domains, INTERPRO:IPR002965;
contains Pfam profile PF00194: Eukaryotic-type carbonic
anhydrase
Length = 350
Score = 30.3 bits (65), Expect = 3.3
Identities = 28/112 (25%), Positives = 33/112 (29%)
Frame = +3
Query: 426 PEETKPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAK 605
P+ KPKP + P P PKP PA P K K PA
Sbjct: 26 PKPPKPKPAPAPTPPKPKPTPAPTPPKPKPKPA----PTPPKPKPAPAPTPPKPKPAPAP 81
Query: 606 VNEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGPSEHVTSKPRKKVDSSG 761
P + KP + KP P P+ KP K G
Sbjct: 82 TPPKPKPKPAPTPPNPKPTPAPTPPKP-KPAPAPAPTPAPKPKPAPKPAPGG 132
Score = 29.5 bits (63), Expect = 5.8
Identities = 26/115 (22%), Positives = 31/115 (26%)
Frame = +3
Query: 426 PEETKPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAK 605
P KPKP + P+P PKP PA K TP
Sbjct: 37 PTPPKPKPTPAPTPPKPKPKPAPTPPKPKPAPAPTPPKPKPAPAPTPPKPKPKPAPTPPN 96
Query: 606 VNEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGPSEHVTSKPRKKVDSSGAAG 770
P K P A KP P P V + ++ G G
Sbjct: 97 PKPTPAPTPPKPKPAPAPAPT-PAPKP-KPAPKPAPGGEVEDETEFSYETKGNKG 149
>At1g26150.1 68414.m03192 protein kinase family protein similar to
Pto kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 30.3 bits (65), Expect = 3.3
Identities = 27/116 (23%), Positives = 41/116 (35%), Gaps = 2/116 (1%)
Frame = +3
Query: 429 EETKPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKV 608
E P P K E+ P P S P P+ P+ PS ++T
Sbjct: 215 EHPSPPPPGHPKRREQPPPPGSKRPTPSP-PS-----PSDSKRPVHPSPPSPPEETLPPP 268
Query: 609 NEVPTEVSEKSASPDKPIDVGSATKPDAP--VTHDGPSEHVTSKPRKKVDSSGAAG 770
P + S+SP + S P +P + GP + P D+S ++G
Sbjct: 269 KPSPDPLPSNSSSPPTLLPPSSVVSPPSPPRKSVSGPDNPSPNNPTPVTDNSSSSG 324
>At5g67470.1 68418.m08507 formin homology 2 domain-containing protein
/ FH2 domain-containing protein contains formin homology
2 domain, Pfam:PF02181
Length = 899
Score = 29.9 bits (64), Expect = 4.4
Identities = 11/28 (39%), Positives = 13/28 (46%)
Frame = -2
Query: 1327 QXPAQTPXXXPPPRAXKXPPPXPP*IGP 1244
+ P P PPP PPP PP + P
Sbjct: 363 KSPVPPPRRSPPPLQTPPPPPPPPPLAP 390
>At1g61080.1 68414.m06877 proline-rich family protein
Length = 907
Score = 29.9 bits (64), Expect = 4.4
Identities = 12/21 (57%), Positives = 12/21 (57%)
Frame = -2
Query: 1318 AQTPXXXPPPRAXKXPPPXPP 1256
A P PPPRA PPP PP
Sbjct: 521 AAPPPPPPPPRAAVAPPPPPP 541
>At1g01730.1 68414.m00092 expressed protein
Length = 224
Score = 29.9 bits (64), Expect = 4.4
Identities = 14/47 (29%), Positives = 21/47 (44%)
Frame = +3
Query: 576 PSKSKDTPAKVNEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGPS 716
P+ + PA+ +PT ++SA P T P + VT D S
Sbjct: 16 PAATSSLPAQAPPLPTSADQRSAELPSPAQPAQMTAPPSTVTTDPSS 62
>At5g66830.1 68418.m08426 F-box family protein contains F-box domain
Pfam:PF00646
Length = 394
Score = 29.5 bits (63), Expect = 5.8
Identities = 20/63 (31%), Positives = 27/63 (42%)
Frame = -1
Query: 416 LSFCWCFFTFGLRYFFFSFWSRIGFLFLFRNGMLCFSGFCVSYRSRLSNQFLFFISDRLR 237
L +CW L F F R+GF R +C S VS S+ +NQ + I
Sbjct: 16 LDWCWSKLPSDLMQFVFD---RLGFADFQRAKSVCSSWLSVSRNSQPNNQIPWMIRFPKD 72
Query: 236 NSH 228
N+H
Sbjct: 73 NNH 75
>At3g50650.1 68416.m05540 scarecrow-like transcription factor 7 (SCL7)
Length = 542
Score = 29.5 bits (63), Expect = 5.8
Identities = 15/44 (34%), Positives = 20/44 (45%)
Frame = -2
Query: 1321 PAQTPXXXPPPRAXKXPPPXPP*IGPXFNLSXKXFHXLFSQLXK 1190
PA T P P + + PPP PP P F+L+ F + K
Sbjct: 146 PASTAIWSPSPPSPQHPPPPPP--QPDFDLNQPIFKAIHDYARK 187
>At3g50180.1 68416.m05486 hypothetical protein
Length = 588
Score = 29.5 bits (63), Expect = 5.8
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = -2
Query: 1315 QTPXXXPPPRAXKXPPPXPP*IGPXFNL 1232
+ P PPP PPP PP +GP L
Sbjct: 23 RAPPPQPPPPPPPPPPPPPPRLGPRLRL 50
>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
(CPK2) identical to calcium-dependent protein kinase
isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 646
Score = 29.5 bits (63), Expect = 5.8
Identities = 21/85 (24%), Positives = 30/85 (35%)
Frame = +3
Query: 432 ETKPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKVN 611
ETK KPD +++ +EK + + E K VP V +S K
Sbjct: 74 ETKLKPDLEIQPEEKKEKVLAEETKQKVVPEESKQEVPPEESKREVVVQPESAKPETKSE 133
Query: 612 EVPTEVSEKSASPDKPIDVGSATKP 686
P ++ S KP KP
Sbjct: 134 SKPETTKPETTSETKPETKAEPQKP 158
>At1g59910.1 68414.m06749 formin homology 2 domain-containing protein
/ FH2 domain-containing protein contains formin homology
2 domain, Pfam:PF02128
Length = 929
Score = 29.5 bits (63), Expect = 5.8
Identities = 13/29 (44%), Positives = 14/29 (48%)
Frame = -2
Query: 1330 GQXPAQTPXXXPPPRAXKXPPPXPP*IGP 1244
G +P PPP A PPP PP GP
Sbjct: 377 GPANQTSPPPPPPPSAAAPPPPPPPKKGP 405
>At5g38560.1 68418.m04662 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 681
Score = 29.1 bits (62), Expect = 7.7
Identities = 26/116 (22%), Positives = 38/116 (32%), Gaps = 4/116 (3%)
Frame = +3
Query: 426 PEETKPKPDDKVKADEKTPEPKSAEPKP----TDVPAXXXXXXXXXXXXXXXXVPSKSKD 593
P T P P +TP P P P P PS +
Sbjct: 128 PTTTNPPPKPSPSPPGETPSPPGETPSPPKPSPSTPTPTTTTSPPPPPATSASPPSSNPT 187
Query: 594 TPAKVNEVPTEVSEKSASPDKPIDVGSATKPDAPVTHDGPSEHVTSKPRKKVDSSG 761
P+ + PT + +KPI KP P +++G + +S P K +G
Sbjct: 188 DPSTLAPPPTPL--PVVPREKPI-----AKPTGPASNNGNNTLPSSSPGKSEVGTG 236
>At5g27120.1 68418.m03237 SAR DNA-binding protein, putative strong
similarity to SAR DNA-binding protein-1 [Pisum sativum]
GI:3132696; contains Pfam profile PF01798: Putative
snoRNA binding domain
Length = 533
Score = 29.1 bits (62), Expect = 7.7
Identities = 19/67 (28%), Positives = 29/67 (43%)
Frame = +3
Query: 429 EETKPKPDDKVKADEKTPEPKSAEPKPTDVPAXXXXXXXXXXXXXXXXVPSKSKDTPAKV 608
EE K + K K+++K E AEP+ + PA +P+K K+ K
Sbjct: 470 EEAKTEEPSKKKSNKKKTE---AEPETAEEPAKKEKKKKRKHEEEETEMPAKKKEKSEKK 526
Query: 609 NEVPTEV 629
+ TEV
Sbjct: 527 KKKKTEV 533
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,010,462
Number of Sequences: 28952
Number of extensions: 401741
Number of successful extensions: 2170
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2027
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3884352576
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -