Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_H01_e488_15.seq
         (1532 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac...   155   1e-38
SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc...   150   6e-37
SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S...    28   3.9  
SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom...    27   5.2  
SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase...    27   5.2  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    27   5.2  
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos...    27   9.1  

>SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein
           S24|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 134

 Score =  155 bits (377), Expect = 1e-38
 Identities = 79/122 (64%), Positives = 93/122 (76%), Gaps = 1/122 (0%)
 Frame = +1

Query: 130 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 309
           MSE   TIRTRKFMTNRLL RKQMV D+LHPGK  +SK EIREKLA+MYK   + V  FG
Sbjct: 1   MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFG 59

Query: 310 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 486
            +T+FGGG+STGFALIYD+ +  KKFEP +RL R G  E  ++  R+QRK+RKNR KKV 
Sbjct: 60  LRTHFGGGRSTGFALIYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVF 119

Query: 487 GT 492
           GT
Sbjct: 120 GT 121


>SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein
           S24|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 134

 Score =  150 bits (363), Expect = 6e-37
 Identities = 74/122 (60%), Positives = 91/122 (74%), Gaps = 1/122 (0%)
 Frame = +1

Query: 130 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 309
           MSE   TIRTRKFMTNRLL RKQMV D+LHPGK  +SK ++REKL +MYK     V  FG
Sbjct: 1   MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFG 59

Query: 310 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 486
            +T++GGG++TGFALIYD ++  KKFEP +RL R G  E  ++  R+QRK+RKNR KKV 
Sbjct: 60  LRTHYGGGRTTGFALIYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVF 119

Query: 487 GT 492
           GT
Sbjct: 120 GT 121


>SPCC417.06c |ppk35|mug27|serine/threonine protein kinase
           Ppk35|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 624

 Score = 27.9 bits (59), Expect = 3.9
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +1

Query: 382 KFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 480
           K+ P  R    G + +KR  RK++ E+KN   K
Sbjct: 539 KYRPNARKPLVGRHREKRQLRKEKPEKKNNSTK 571


>SPAC22A12.11 |dak1||dihydroxyacetone kinase
           Dak1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 580

 Score = 27.5 bits (58), Expect = 5.2
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +1

Query: 247 EIREKLAKMYKVTPDVVFVFGFKTNFGG 330
           +++E LAK YK+ P  +F   F T+  G
Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNG 313


>SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 444

 Score = 27.5 bits (58), Expect = 5.2
 Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
 Frame = -3

Query: 318 RLETEHENY-VWRHFVHFSELFTDLSFADGRFSWMQDIAYHL 196
           RLE EH    V R+      L    +F D R+ W Q+I+  L
Sbjct: 261 RLEVEHPGEEVLRNNRILGRLMPSRAFGDARYKWSQEISERL 302


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 27.5 bits (58), Expect = 5.2
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
 Frame = +1

Query: 610 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALXN 774
           P  P+ S ++ H   +    +    +L N I+L      ++PLSP    A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231

Query: 775 SCA 783
           S A
Sbjct: 232 SDA 234


>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 26.6 bits (56), Expect = 9.1
 Identities = 11/26 (42%), Positives = 18/26 (69%)
 Frame = +2

Query: 422 MKRRGRHANSVRNVRTE*RRYEVPRN 499
           +KRR R  N++R +  E RRY +P++
Sbjct: 28  VKRRKRLFNAIRKLWNERRRYRMPKD 53


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,584,328
Number of Sequences: 5004
Number of extensions: 81339
Number of successful extensions: 193
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 860263516
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)